Chemical Derivatization Processes Applied to Amine Determination in Samples of Different Matrix Composition

A new derivatization reagent for determination of biogenic amines in wines

“…wine samples (Guo et al, 2015). Due to the structural characteristics of BAs, their derivatization is usually needed and typical reagents for the amino group include o-phthaldialdehyde (OPA), 9-fluorenylmethylchloroformate, 2,4,6-trinitrobenzenesulfonic acid (TNBS), diethyl ethoxymethylenemalonate (DEEMM), 4-fluoro-3-dinitro-fluoromethylbenzene and dabsyl-chloride (DABS-Cl) (Guo et al, 2015;Gómez-Alonso et al, 2007;Hernández-Cassou and Saurina, 2011;Płotka-Wasylka et al, 2015;Wang et al, 2014) It should be noted, that International Organization of Vine and Wine (OIV) proposed derivatization with OPA and DEEMM for biogenic amines determination in musts and wine using fluorimetric (OIV-MA-AS315-18) and spectrophotometric (OIV-MA-AS315-26) detection methods (http://www.oiv.int/oiv/info/enmethodesinternationalesvin#autres). However, their application is often affected by restrictions or disadvantages, e.g.…”

Quantitative analysis of biogenic polyamines in distilled drinks by direct electrospray ionization tandem mass spectrometry using a nanocontainer

“…Biogenic polyamines do not significantly absorb above 250 nm making their direct analysis using optical detection difficult to achieve. Most frequently used analytical methods involve derivatization followed by gas chromatography/mass spectrometry (GC/MS) or high‐performance liquid chromatography (HPLC) analysis . As most drinks are relatively complex matrices, the analytical procedures usually also involve lengthy clean‐up and pre‐concentration procedures to improve selectivity and sensitivity.…”

“…Most frequently used analytical methods involve derivatization followed by gas chromatography/mass spectrometry (GC/ MS) or high-performance liquid chromatography (HPLC) analysis. [9][10][11][12][13]15,16] As most drinks are relatively complex matrices, the analytical procedures usually also involve lengthy clean-up and pre-concentration procedures to improve selectivity and sensitivity. Simple and fast methods for the detection and quantification of polyamines in beverages are therefore desirable.…”

Differentiation of isomeric methylanilines by imidization and gas chromatography/mass spectrometry analysis

“…Derivatization has been verified as an effective pre‐treatment method in analytical chemistry, which provides a promising strategy for the solution of many analytical problems . Amidation of the amino group was reported for differentiation of the ortho ‐haloanilines from the meta ‐ and the para ‐ones by electron ionization (EI)‐MS analysis, but it failed to differentiate the meta‐ from the para‐ isomers .…”