Chemical identification and quality evaluation of Lycopus lucidus Turcz by UHPLC‐Q‐TOF‐MS and HPLC‐MS/MS and hierarchical clustering analysis

Abstract

Lycopus lucidus Turcz has been used as a traditional phytomedicine for menstrual disorder, amenorrhea, menstrual cramps, inflammation and cardiovascular diseases. However, there is not enough information about identification and quantification for the chemical constituents of L. lucidus Turcz. In this work, a simple, rapid and sensitive UHPLC-Q-TOF-MS method was developed for characterization and identification of the phytochemical compositions in L. lucidus Turcz in negative ion mode. A total of 37 compounds, including 15 phenolic acids, 12 flavonoids, three triterpenoids and seven organic acids were tentatively characterized and identified by means of the retention time, accurate mass and characteristic fragment ions. Thirteen compounds were reported for the first time in L. lucidus Turcz. Among of them, 11 compounds were further quantified by multiple reactions monitoring. The results showed good performance with respect to linearity (r > 0.9959), repeatability (RSD < 2.6%), intra- and inter-day precision (RSD < 3.2%), recovery (93.1-104.9%), and lower limit of quantification (5-50 ng/mL). Subsequently, the results were analyzed and classified by hierarchical cluster analysis. The research could be applied for identification and quality evaluation for L. lucidus Turcz.

A Comparative Metabolomics Analysis Reveals the Tissue-Specific Phenolic Profiling in Two Acanthopanax Species

“…Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been used in systems analysis of complicated metabolome [ 24 , 28 , 29 ]. A total of 37 compounds in Lycopus lucidus Turcz, including 15 phenolic acids, 12 flavonoids, three triterpenoids and seven organic acids were tentatively characterized and identified by means of the UHPLC-Q-TOF-MS method [ 30 ]. In the same way, 42 polymethoxylated flavonoids, including 33 flavones and nine flavanones, were distinguished in Citrus reticulata and Citrus sinensis [ 30 , 31 ].…”

“…A total of 37 compounds in Lycopus lucidus Turcz, including 15 phenolic acids, 12 flavonoids, three triterpenoids and seven organic acids were tentatively characterized and identified by means of the UHPLC-Q-TOF-MS method [ 30 ]. In the same way, 42 polymethoxylated flavonoids, including 33 flavones and nine flavanones, were distinguished in Citrus reticulata and Citrus sinensis [ 30 , 31 ]. This method was previously applied in Acanthopanax senticosus plants for the simultaneous determination of five major bioactive constituents (eleutheroside B, chlorogenic acid, caffeic acid, eleutheroside E and isofraxidin) [ 11 ].…”

Comprehensive profiling of Lingzhihuang capsule by liquid chromatography coupled with mass spectrometry-based molecular networking and target prediction

“…In this study, the chemical constituents identified or tentatively characterized in LZHC were mainly triterpenoids and flavonoids, and triterpenoids accounted for the majority. Phenolic acids such as protocatechuic acid, chlorogenic acid, and caffeic acid have significant anti-inflammatory, anti-oxidant, and anti-viral activities [32][33][34] . Likewise, flavonoids including liquiritin, liquiritigenin, and licochalcone A have anti-inflammatory, immunomodulatory, anti-tumor, and anti-bacterial effects [35][36][37] .…”

The Potential of Lamiaceae Herbs for Mitigation of Overweight, Obesity, and Fatty Liver: Studies and Perspectives

“…In Europe, we find the species Lycopus europaeus L. and Lycopus exaltatus L.f. The leaves are rich in flavonoids, coumarins, terpenoids, and tannins [ 129 ], and they have use in treating edema, wound healing, T2DM, and pain, especially in traditional Chinese medicine. L. lucidus has been suggested to play a protective role against obesity, NAFLD, and metabolic diseases.…”