Chemical Modification and Characterization of Cotton Pulp as Cationic Material for Protiens Separation

Abstract

The present study aims to chemically modify low-cost polymer to value-added products and to test the newly synthesized material efficacy for protein purification. Different procedures were used for the introduction of anionic and cationic exchangers to the un-modified materials. The study focus on modification of cellulose present in cotton pulp. The material was chemically modified by with glycidyltrimethyl ammonium chloride (GTMAC) and 2,2,6,6-tetramethylpipiridine 1-oxyl radical (TEMPO) to enhance their binding capacities with different proteins. The physical and chemical properties of unmodified biopolymers and modified biopolymers were characterized by degree of swelling, extent of modification, Scanning electron microscopy (SEM), Energy dispersive X- Ray (EDX), Fourier transform infrared spectroscopy (FTIR). Molecular interaction studies were done in MOE using triangle matcher and refinement approaches. To see the binding capacity of the modified materials sorption experiments were performed using spectrometer. Lysozyme and Bovine serum albumin were used to check the binding properties of the modified substances. Very reasonable binding capability for proteins for found.