Chemical profiles and quality evaluation of Buddleja officinalis flowers by HPLC-DAD and HPLC-Q-TOF-MS/MS

GuangHui, Xie; Xu, Qiang; Li, Ran; Shi, Lu; Han, Yu; Yu, Zhu; Wu, Gang; Qin, Min-Jian

doi:10.1016/j.jpba.2018.10.030

Cited by 40 publications

(15 citation statements)

References 64 publications

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“…Identification of 5‐caffeoylquinic acid (peak 3) was confirmed by comparison with retention time of a commercial standard; however, the identity of caffeoylquinic acid isomer (peak 2) was not confirmed as a result of a lack of coincidence of the retention time of the peak with the available standards 3‐caffeoylquinic and 4‐caffeoylquinic acid. Identification of the quercetin‐hexoside (peak 5) isomer was also not confirmed as a result of a lack of coincidence with the retention time of the commercial standard quercetin‐3‐glucoside; for this reason, this compound may possibly correspond to quercetin‐5‐glucoside or quercetin‐7‐glucoside, all with similar fragmentation patterns characterized by a pseudomolecular ion with m / z 463 and a product ion of m / z 301 in the negative ion mode . It is important to consider that glucoside and galactoside derivatives present similar fragmentation patterns by MS, characterized by a neutral loss to m / z 162; however, 3‐galactoside was discarded as a result of the elution order, ecause galactoside derivatives must eluted at lower retention times than glucoside derivatives .…”

Section: Resultsmentioning

confidence: 99%

Efficiency of two arbuscular mycorrhizal fungal inocula to improve saline stress tolerance in lettuce plants by changes of antioxidant defense mechanisms

et al. 2019

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BACKGROUND: Arbuscular mycorrhizal (AM) fungi establish symbioses with most agricultural plants and improves growthunder soil stress conditions. The present study aimed to evaluate the functional contribution of 2 AM fungal inocula (a native consortium isolated from saline soils of the Atacama Desert, 'HMC', and a reference inoculum Claroideoglomus claroideum, 'Cc') on the growth and antioxidant compounds of two cultivars of lettuce (Lactuca sativa cvs. 'Grand Rapids' and 'Lollo Bionda') at increasing salt stress conditions (0, 40, and 80 mmol L -1 NaCl). At 60 days of plant growth, the symbiotic development, biomass production, lipid peroxidation, proline content, antioxidant enzymes, phenolic compound profiles and antioxidant activity were evaluated. RESULTS: The 2 AM inocula differentially colonized the roots of Grand Rapids and Lollo Bionda lettuce plants. The AM symbioses increased proline synthesis and superoxide dismutase, catalase and ascorbate peroxidase activities and diminished phenolic compound synthesis and oxidative damage in lettuce, which was related positively to a higher growth of inoculated plants under salt exposure. The higher concentration of phenolic compounds induced by salinity in non-inoculated plants was associated with high oxidative stress and low fresh biomass production.CONCLUSION: Modulation of salinity stress in lettuce by AM root colonization is a result of changes of antioxidant enzymatic systems that reduce oxidative damage and sustain growth. The application of AM fungi to improve crop production by means of directed inoculation with efficient AM fungal strains may enhance lettuce production on soils plagued with salinity worldwide.

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Section: Resultsmentioning

confidence: 99%

Efficiency of two arbuscular mycorrhizal fungal inocula to improve saline stress tolerance in lettuce plants by changes of antioxidant defense mechanisms

et al. 2019

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“…Compound 122, as shown in Figure S10 (supporting information), exhibited quasi-molecular ion at 2390, 420.2283, 292.2012, 204.1013, and 147.0440 could be observed. It was tentatively identified as safflospermidine B, referring to the literature (Xie et al, 2019). In addition, three isomers of safflospermidine B were detected and identified as N 1 (Z),N 5 (Z),N 10 (E)-tri-p-coumaroyl spermidine, N 1 (E),N 5 (Z),N 10 (E)-trip-coumaroylspermidine, and N 1 ,N 5 ,N 10 (E)-tri-p-coumaroylspermidine from CF (Xie et al, 2019).…”

Section: Identification Of Spermidine Alkaloidsmentioning

confidence: 99%

Systematic characterization of the chemical constituents in vitro and prototypes in vivo of Dingkun Dan using ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry combined with the UNIFI™ software

Dou

Lin

Tian

et al. 2020

Biomedical Chromatography

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Dingkun Dan (DKD), a famous traditional Chinese medicine, has been widely used in the treatment of irregular menstruation, leucorrhea abnormality, and postpartum gynecological diseases since Qing dynasty (1739). It comprises 30 flavors of Chinese medicinal materials, which results in its complex chemical composition. In this study, an integrative method was developed to rapidly characterize the chemical components of DKD using ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry combined with the UNIFI™ software. A total of 234 compounds, including 47 triterpenoid saponins, 55 flavonoids, and 38 alkaloids, were identified. Of them, 170 compounds were characterized initially and 61 compounds were identified unambiguously using reference standards. Under the same analysis conditions, 43 prototypical components, which were tentatively assigned as triterpenoid saponins, flavonoids, alkaloids, terpenoids, phenylpropanoids, and others, were absorbed in rat by serum pharmacochemistry analysis. DKD exhibited diverse pharmacological activities through the combined effect of these components. This study was the first systematic study of chemical components in vitro originating from 30 medicinal materials and prototypes in vivo of DKD, which could provide scientific evidence for explaining its therapeutic effect.

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“…Fortunately, multi-component quantitative analysis has been developed as a reliable method to control the quality of TCM [15,16,17]. In addition, HPLC-ESI-Q-TOF-MS/MS and HPLC fingerprint combined with chemometrics as an effective, reliable and comprehensive method were also successfully applied for quality consistency assessment of TCM [18,19,20]. Therefore, the simultaneous qualitative and quantitative of TCM can be achieved by the combination of HPLC-ESI-Q-TOF-MS/MS, HPLC fingerprint and multicomponent quantitative analysis.…”

Section: Introductionmentioning

confidence: 99%

Chemical Profile and Multicomponent Quantitative Analysis for the Quality Evaluation of Toad Venom from Different Origins

Cao

Pan

et al. 2019

Molecules

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Toad venom (Chansu), a traditional Chinese medicine (TCM), has been widely used for treating various cancer. However, it is considerably difficult to evaluate the quality of Chansu due to its complex chemical compositions. Hence, finding the characteristic ingredients and developing a scientific and comprehensive quality evaluation method are essential for guaranteeing the safety and efficacy of Chansu. In this paper, the chemical composition database of Chansu was successfully established and HPLC-ESI-Q-TOF-MS/MS was applied for chemical profiling of the ingredients in Chansu. In total, 157 compounds were identified, including 22 amino acids, 8 alkaloids, 54 bufogenins, 63 bufotoxins, and 10 other compounds. Furthermore, HPLC fingerprints and quantitative analysis of its multicomponent were successfully developed to evaluate the quality consistency of Chansu from different origins. The results suggested that the HPLC fingerprint of Chansu could be divided into an amino acid and alkaloid region, as well as a bufogenins and bufotoxins region. The fingerprint profile of Chansu from different geographical origins were different, indicating that its quality was affected by the geographical factors. In addition, seven characteristic peaks were selected as the quantitative markers to evaluate the quality of the Chansu. The Kruskal–Wallis test illustrated that the contents of seven bufogenins in Chansu were significantly (p < 0.01) different among different origins. The total contents of the seven compounds ranged from 100.40 to 169.22 mg/g in 20 batches of Chansu samples. This study demonstrated that integrating HPLC-ESI-Q-TOF-MS/MS, HPLC fingerprints, and multicomponent quantitative analysis coupled with chemometrics was a comprehensive and reliable strategy for evaluation of Chansu in both qualitative and quantitative aspects. In addition, our study represented the most comprehensive characterization on the chemical compositions of Chansu, which could provide important reference information for the discovery of potential bioactive compounds.

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Chemical profiles and quality evaluation of Buddleja officinalis flowers by HPLC-DAD and HPLC-Q-TOF-MS/MS

Cited by 40 publications

References 64 publications

Efficiency of two arbuscular mycorrhizal fungal inocula to improve saline stress tolerance in lettuce plants by changes of antioxidant defense mechanisms

Efficiency of two arbuscular mycorrhizal fungal inocula to improve saline stress tolerance in lettuce plants by changes of antioxidant defense mechanisms

Systematic characterization of the chemical constituents in vitro and prototypes in vivo of Dingkun Dan using ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry combined with the UNIFI™ software

Chemical Profile and Multicomponent Quantitative Analysis for the Quality Evaluation of Toad Venom from Different Origins

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