Chemical quantification and antioxidant assay of four active components in Ficus hirtaroot using UPLC-PAD-MS fingerprinting combined with cluster analysis

Yi, Tao; Chen, Qilei; He, Xi-Cheng; So, Suiwai; Lo, Yuenling; Fan, Lan-Lan; Xu, Jun; Tang, Yiqun; Zhang, Jianye; Zhao, Zhongzhen; Chen, Hubiao

doi:10.1186/1752-153x-7-115

Cited by 58 publications

(37 citation statements)

References 21 publications

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“…Peak of cnidilide, ligustilide, ligustrazine and ferulic acid were associated with significant pharmacological activities and the compounds could significantly inhibit myocardial ischemia caused by coronary artery ligation. The pharmacological effects of this kind of the extract are consistent with previous studies [38][39][40][41]. Ligustrazine can significantly reduce serum lactic acid and ferulic acid, the rise of chaff this can significantly reduce serum FFA lactone is the main active ingredient of the traditional Chinese medicine Chuanxiong…”

Section: Analysis Of Spectrum-effectsupporting

confidence: 77%

Screening of Bioactive Ingredients in Ligusticum Chuanxiong Hort for Protection against Myocardial Ischemia

Liu

Song-gang

et al. 2016

Cell Physiol Biochem

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Background/Aims: To study the spectrum-effect relationship and effective components of Ligusticum Chuanxiong Hort. (LCH) on the protection of canine myocardial ischemia. Methods: Fingerprint spectrum of LCH extracts was developed using high performance liquid chromatography (HPLC), and a canine model of acute myocardial ischemia was established by ligating the coronary artery. Bivariate correlation analysis and multivariate regression analysis were used to correlate the pharmacodynamics of LCH extract and its common peaks in HPLC. Results: The bioactive components of LCH were ligustrazine, ferulic acid, cnidilide and ligustilide. Ligustrazine and ferulic acid could significantly reduce serum lactic acid in canine model of acute myocardial ischemia, while ligustilide could significantly reduce the elevation of serum free fatty acid. Conclusions: The spectrum-effect relationship study shows that the effective components of LCH are ligustrazine, ferulic acid, cnidilide and ligustilide, which have protective effect on myocardial ischemia.

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Section: Analysis Of Spectrum-effectsupporting

confidence: 77%

Screening of Bioactive Ingredients in Ligusticum Chuanxiong Hort for Protection against Myocardial Ischemia

Liu

Song-gang

et al. 2016

Cell Physiol Biochem

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“…This permit the explanation of 100% variance (Liebig et al, 1992). Hierarchical cluster analysis (HCA) involves a measurement of the similarity between objects to be clustered, and samples with the maximum similarities were clustered preferentially (Yi et al, 2013). In this study, the ten determined components of the 54 honey samples was inputted into STAT as variables, between group average linkage method was applied to sort honey types into groups, and rescaled distance was selected as measurement to obtain a HCA dendrogram shown in Figure 4.…”

Section: Principal Component Analysis and Hierarchical Cluster Analysismentioning

confidence: 99%

Characterization of Turkish honeys regarding of physicochemical properties, and their adulteration analysis

Kıvrak

Karababa

2016

Food Sci. Technol

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This work was conducted to evaluate the quality of 54 honey samples from eighteen different origins from Turkey. Physicochemical properties were examined according to ADAC methods, total phenolic and flavonoid contents by a spectrophotometric method and authenticity of honeys by Combustion Module -Cavity Ring-Down Spectroscopy (CM-CRDS). The microscopic analysis of honey sediment (mellissopalynology) was carried out to identify and count the pollen to provide qualitative indicators to confirm botanical origin. The moisture, electrical conductivity and free acidity of honeys ranged from 15.56 to 18.39%, 0.143 to 2.006 mS.cm . The highest proline was determined in thyme honey. The results showed that honeys contained eminent amounts of phenolics and flavonoids. δ 13 C values of honeys were more negative than -23.5‰. The C 4 % sugar ratios were lower than 7% value. The lowest glucose-fructose content was observed in eucalyptus, cedar and pine honey samples. The results obtained for physicochemical characteristics, total phenolic and flavonoid contents an authenticity analysis of Turkish honeys indicate a good quality level, adequate processing, good maturity and freshness. The discrimination between honey types was achieved by PCA.Keywords: Combustion Module-Cavity Ring-Down Spectroscopy; C 4 sugar; unifloral honeys; diastase; proline Practical Application: Honey market is a large part of the economy for many countries. Unfortunately due to large market profit for honey sales, adulteration is extremely widespread. This study indicated the results of analysis of the reliable and high-throughput instrument Combustion Module-Cavity Ring-Down Spectroscopy (CM-CRDS) for the determination of C 4 sugar adulteration in honey for the first time. We consider that CM-CRDS will be useful for food control and analysis. Additionally, the present research furnished a detailed qualification of honey samples from eighteen different Turkish origins in terms of chemical and physicochemical properties.

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“…The DPPH free radical-scavenging activity of each sample was assayed according to published articles [14–16] with minor modifications. In brief, a 0.3 mM solution of methanolic DPPH was freshly prepared.…”

Section: Methodsmentioning

confidence: 99%

“…The ability to reduce ferric ions was measured based on the methods reported in previous papers [16, 17] with minor revisions. Briefly, 30 μL of each sample (with appropriate enrichment or dilution if necessary) was added to 200 μL of FRAP reagent (10 parts of 300 mM sodium acetate buffer at pH 3.6, 1 part of 10.0 mM 2,4,6-tripyridyl-s-triazine (TPTZ) solution, and 1 part of 20.0 mM FeCl 3 ·6H 2 O solution), and the reaction mixture was placed at room temperature for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Chemical compositions, chromatographic fingerprints and antioxidant activities of Citri Exocarpium Rubrum (Juhong)

et al. 2017

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BackgroundCitri Exocarpium Rubrum (CER), which is known as Juhong in Chinese, is the dried exocarp of Citrus reticulata Blanco and its cultivars (Fam. Rutaceae) and is currently used in Chinese medicine to protect the stomach and eliminate dampness and phlegm. The main aim of this study was to develop a high-performance liquid chromatography ultraviolet mass spectrometry (HPLC-UV-MS) method for determining the chemical compositions and fingerprint of CER. We also evaluated the antioxidant properties of CER based on its 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, ferric ion reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) assays.MethodsTen CER samples were collected from Hong Kong and mainland China. Each CER sample was extracted using an ultrasonic extraction method. Chromatographic separation was achieved using a conventional Dikma Inspire C18 column with photo diode array detection (190–400 nm). Hesperidin, nobiletin and tangeretin were quantified based on the UV signal observed at 330 nm. The column was eluted with a mobile phase consisting of water and acetonitrile (15–55%) over 55 min. Fingerprints combined with similarity and principal component analyses were used to classify the herbs. The DPPH free radical scavenging activity, FRAP and ABTS properties of the different CER samples were assayed. Bivariate correlation analysis was performed to investigate the correlation between the characteristic peaks and their antioxidant capacities.ResultsLimit of detection (LOD), limit of quantification (LOQ), linearity, inter-day precision, intra-day precision, repeatability, stability and recovery of the developed method were validated, and the method was subsequently used to determine the contents of hesperidin, nobiletin and tangeretin, and to acquire the fingerprints of the CER samples. Seventeen characteristic peaks were found in the fingerprints, and eleven of them were identified. Bivariate correlation analysis revealed correlations between the characteristic peaks and the antioxidant activities of the samples.ConclusionAn HPLC-UV-MS method was developed and validated after a detailed investigation on extraction of chemical compounds from CER using different solvents and extraction times. None of the peaks was correlated with the DPPH free radical scavenging activity or ferric reducing capacity. Most of the peaks were correlated well with the ABTS radical scavenging capacity.Electronic supplementary materialThe online version of this article (doi:10.1186/s13020-017-0127-z) contains supplementary material, which is available to authorized users.

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Chemical quantification and antioxidant assay of four active components in Ficus hirtaroot using UPLC-PAD-MS fingerprinting combined with cluster analysis

Cited by 58 publications

References 21 publications

Screening of Bioactive Ingredients in Ligusticum Chuanxiong Hort for Protection against Myocardial Ischemia

Screening of Bioactive Ingredients in Ligusticum Chuanxiong Hort for Protection against Myocardial Ischemia

Characterization of Turkish honeys regarding of physicochemical properties, and their adulteration analysis

Chemical compositions, chromatographic fingerprints and antioxidant activities of Citri Exocarpium Rubrum (Juhong)

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