Chemical shift imaging: a review

Brateman, Libby

doi:10.2214/ajr.146.5.971

Cited by 88 publications

(37 citation statements)

References 2 publications

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“…Conversely, tissue without elevated lipids but demonstrating a striking decrease in the creatine resonances could be indicative of irreversibly damaged tissue. With the evolution of chemical-shift imaging methods and their applications (19), 'H NMR methods should be clinically useful to study ischemic heart disease, and our work provides the NMR spectroscopic background for such studies regarding this important clinical problem.…”

Section: Discussionmentioning

confidence: 99%

Proton NMR spectroscopy in myocardial ischemic insult

Evanochko

Reeves

Sakai

et al. 1987

Magnetic Resonance in Med

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Proton NMR methods can monitor mobile lipids (e.g., fatty acids and glycerides) in intact tissue. Lipids play a major role in cardiac energy production, and elevated levels of myocardial lipids have been reported following an ischemic insult. The present study examines the potential of high-resolution 1H NMR spectroscopy to monitor lipid alterations 24 h following coronary occlusion in dogs, and to correlate these finds with regional myocardial blood flow (RMBF) measured by radiolabeled microspheres. The dogs were killed, and samples of excised myocardium were studied by high-resolution 1H NMR spectroscopic analysis using solvent suppression in combination with the Hahn spin-echo pulse sequence. Mobile lipids levels in myocardium with moderate blood flow reduction (28.6 +/- 7, integral values, arbitrary units; flow 5-50% of control) were significantly elevated compared to the mobile lipid levels in control myocardium (5.3 +/- 8, P less than 0.001) and in myocardium with severe flow reduction (7.2 +/- 10, P less than 0.001; flow less than 5% of control). The mobile lipids in myocardium with severe flow reduction were not elevated significantly relative to control tissue. As anticipated, depression in the level of creatine paralleled the microsphere determined degree of ischemia, i.e., compared to control (9.0 +/- 3); creatine levels were moderately decreased with flows 5-50% of control (5.5 +/- 4, P less than 0.001) and markedly decreased with flows less than 5% of control (1.0 +/- 2, P less than 0.001). This study suggests that high-resolution 1H NMR spectroscopy may be used to evaluate alterations in myocardial lipid levels following an ischemic insult.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Proton NMR spectroscopy in myocardial ischemic insult

Evanochko

Reeves

Sakai

et al. 1987

Magnetic Resonance in Med

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“…Several fat-suppression techniques for MR imaging have been described [2,3]. In the selective excitation (suppression) method, a frequency-specific preexcitation pulse is used to excite fat protons selectively.…”

Section: Fat-suppression Techniquesmentioning

confidence: 99%

MR imaging of the spine: recent advances in pulse sequences and special techniques.

Georgy¹,

Hesselink²

1994

American Journal of Roentgenology

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“…The first category is for measuring biophysical or physiological parameters, such a.s molecular diffusion or tissue perfusion (1)(2)(3)(4)(5). The second category is for identifying the distribution of specific molecules of biomedical interest by spectroscopic MRI or chemical shift imaging (6,7). The third category uses labels or tracers for MRI to investigate the normal cellular function and pathological processes of specific cells or organs (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Intracellular labeling of T‐cells with superparamagnetic contrast agents

Yeh

Zhang

Ildstad

et al. 1993

Magnetic Resonance in Med

189

131

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Isolated rat T-cells have been labeled intracellularly, using endocytosis uptake of two superparamagnetic contrast agents, AquaMag100 and BMS180549, which are both iron-oxide particles coated with dextran. No deterioration of cell proliferation response to mitogen stimulation was observed after labeling with either superparamagnetic contrast agent. AquaMag100 particles show aggregation and co-precipitation in culture media for T-cells. BMS180549 particles not only produce no observable aggregation or co-precipitation, but also have a higher efficiency for labeling T-cells than AquaMag100. The efficiency of cell labeling was determined by measuring the decrease in the spin-spin relaxation time of the water proton in cell samples containing 1 x 10(7) labeled T-cells/milliliter of 2% w/w gelatin. After optimization of the labeling procedures, a shortening of the spin-spin relaxation time by a factor of approximately 7 to 10 has been demonstrated. Under the present experimental conditions, the up-regulation of low density lipoprotein receptor does not increase the labeling efficiency by endocytosis. Our results suggest that intracellular labeling of specific cell types can be achieved with good efficiency and the labeled cells can be detected by magnetic resonance imaging in rat testicles in vivo.

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Chemical shift imaging: a review

Cited by 88 publications

References 2 publications

Proton NMR spectroscopy in myocardial ischemic insult

Proton NMR spectroscopy in myocardial ischemic insult

MR imaging of the spine: recent advances in pulse sequences and special techniques.

Intracellular labeling of T‐cells with superparamagnetic contrast agents

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