Chemical Structure of Stabilizing Layers of Negatively Charged Silver Nanoparticles as an Effector of Shifts in Soil Bacterial Microbiome under Short-Term Exposure

Abstract

In this work, we have assessed the exposure of soil bacteria from potato monoculture to three types of silver nanoparticles (AgNPs) as well as silver ions (Ag+ ions) delivered in the form of silver nitrate and a commercially available fungicide. The diversity of the soil microbial community, enzymatic activity, and carbon source utilization were evaluated. It was found that only the fungicide significantly limited the abundance and activity of soil bacteria. Silver ions significantly reduced bacterial metabolic activity. In turn, one type of AgNPs prepared with the use of tannic acid (TA) increased bacterial load and activity. There was found in all AgNPs treated soils (1) a greater proportion of all types of persistent bacteria, i.e., Bacillus, Paenibacillus, and Clostridium; (2) a visible decrease in the proportion of Nocardioides, Arthrobacter, and Candidatus Solibacter; (3) almost complete depletion of Pseudomonas; (4) increase in the number of low-frequency taxa and decrease in dominant taxa compared to the control soil. Despite the general trend of qualitative changes in the bacterial community, it was found that the differences in the chemical structure of the AgNP stabilizing layers had a significant impact on the specific metabolic activity resulting from qualitative changes in the microbiome.

Statistical optimization for greener synthesis of multi-efficient silver nanoparticles from the Hypocrea lixii GGRK4 culture filtrate and their ecofriendly applications

“…Hence, a possible mechanism of antibacterial activity is shown in Figure 8. Several recent studies reported and supported the antibacterial activity of synthesized AgNPs (Przemieniecki et al, 2022;Tripathi and Goshisht, 2022;Ivankovic et al, 2023).…”

The Antibacterial Activity of Biosynthesized Silver Nanoparticles from Tribulus terrestris Against Pseudomonas aeruginosa

“…The silver ion's positive charge is assumed to be responsible for its antibacterial effect, as it attracts negatively charged microbes via electrostatic contact. This attraction is likely to outweigh other variables that influence bacterial cell death, such as size and shape [27].…”

Silver Is Not Equal to Silver: Synthesis and Evaluation of Silver Nanoparticles with Low Biological Activity, and Their Incorporation into C12Alanine-Based Hydrogel

“…The weight concentration of silver (Ag) nanoparticles in the solution after purification was evaluated by measuring the density of the native suspension ρ s and the effluent ρ e obtained following centrifugation. The mass concentration is then determined using the formula [ 54 ]: where: is the specific density of silver nanoparticles [ 55 ].…”