Chemically modified polymeric resins for separation of cations, organic acids, and small polar moleculea by high performance liquid chromatography

Abstract

This dissertation starts with a general introduction containing a literature review. This is followed by two research papers which have been published in analytical chemistry journals and a third which will soon be submitted for publication. Permission from the publisher extending reproduction and distribution rights is acknowledged. Each paper is preceded by a summary and followed by conclusions. The three papers are followed by a general conclusions section. References cited in the general introduction are compiled in the literature-cited section following general conclusions. Each paper appears in its entirety including references and figures. Figures are contained in the text of the paper at the appropriate location. References cited within each paper are listed after the conclusions of each paper. High Performance Liquid Chromatography High performance liquid chromatography (HPLC) has grown tremendously since its inception in the early 1970's. Although gas chromatographs were available in the 1960's, there was an obvious need for an instrumental technique that could separate water soluble, thermally labile, non-volatile compounds with speed, precision, and high resolution. The development of HPLC was stimulated by the discovery of DNA. A reliable technique was needed to separate and quantify small amounts of nucleotides and nucleosides from the hydrolysates of DNA and RNA (1). Since its early beginnings, HPLC has expanded to include more specific modes of operation such as adsorption, ion-exchange, normal-phase, reversed-phase, size-exclusion, ion-exclusion,