Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering

Mansfield, Jessica C.; Moger, Julian; Green, Ellen; Moger, C.J.; Winlove, C. Peter

doi:10.1002/jbio.201200213

Cited by 36 publications

(45 citation statements)

References 40 publications

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“…A high amount of chondroitin sulfate produced by the chondrocyte is seen around it, whereas DNA is found inside the chondrocyte. ) and the phosphate (959 cm ¡1 ) peaks, clearly differentiated non-calcified cartilage from the calcified cartilage (71).…”

Section: ¡1mentioning

confidence: 91%

Vibrational spectroscopy of articular cartilage

Rieppo

Töyräs

Saarakkala

2016

Applied Spectroscopy Reviews

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Articular cartilage is a connective tissue that is located at the ends of long bones. Type II collagen, proteoglycans, water, and chondrocytes are the main constituents of articular cartilage. Osteoarthritis, the most common joint disease in the world, causes degenerative changes in articular cartilage tissue. Fourier transform infrared, Raman, and near infrared spectroscopic techniques offer versatile tools to assess biochemical composition and quality of articular cartilage. These vibrational spectroscopic techniques can be used to broaden our understanding about the compositional changes during osteoarthritis, and they also hold promise in disease diagnostics. In this article, the current literature of articular cartilage spectroscopic studies is reviewed.

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Section: ¡1mentioning

confidence: 91%

Vibrational spectroscopy of articular cartilage

Rieppo

Töyräs

Saarakkala

2016

Applied Spectroscopy Reviews

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“…17−24 Raman spectroscopy is a vibrational light scattering technique that can provide a fingerprint of the biochemical composition of cells and tissues. 25 When incident laser light irradiates a sample, a small fraction of the photons (∼1 in 10 8 ) is scattered inelastically with wavelength shifts corresponding to the Raman active normal modes of the molecules.…”

Section: Introductionmentioning

confidence: 99%

Raman Spectroscopy Reveals New Insights into the Zonal Organization of Native and Tissue-Engineered Articular Cartilage

et al. 2016

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Tissue architecture is intimately linked with its functions, and loss of tissue organization is often associated with pathologies. The intricate depth-dependent extracellular matrix (ECM) arrangement in articular cartilage is critical to its biomechanical functions. In this study, we developed a Raman spectroscopic imaging approach to gain new insight into the depth-dependent arrangement of native and tissue-engineered articular cartilage using bovine tissues and cells. Our results revealed previously unreported tissue complexity into at least six zones above the tidemark based on a principal component analysis and k-means clustering analysis of the distribution and orientation of the main ECM components. Correlation of nanoindentation and Raman spectroscopic data suggested that the biomechanics across the tissue depth are influenced by ECM microstructure rather than composition. Further, Raman spectroscopy together with multivariate analysis revealed changes in the collagen, glycosaminoglycan, and water distributions in tissue-engineered constructs over time. These changes were assessed using simple metrics that promise to instruct efforts toward the regeneration of a broad range of tissues with native zonal complexity and functional performance.

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“…These techniques have been more frequently applied to bone, cartilage, tendon, and ligament than the entheses. [11,41,186,187] …”

Section: Construct Maturationmentioning

confidence: 99%

Next generation tissue engineering of orthopedic soft tissue-to-bone interfaces

et al. 2017

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Soft tissue-to-bone interfaces are complex structures that consist of gradients of extracellular matrix materials, cell phenotypes, and biochemical signals. These interfaces, called entheses for ligaments, tendons, and the meniscus, are crucial to joint function, transferring mechanical loads and stabilizing orthopedic joints. When injuries occur to connected soft tissue, the enthesis must be re-established to restore function, but due to structural complexity, repair has proven challenging. Tissue engineering offers a promising solution for regenerating these tissues. This prospective review discusses methodologies for tissue engineering the enthesis, outlined in three key design inputs: materials processing methods, cellular contributions, and biochemical factors.

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Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering

Cited by 36 publications

References 40 publications

Vibrational spectroscopy of articular cartilage

Vibrational spectroscopy of articular cartilage

Raman Spectroscopy Reveals New Insights into the Zonal Organization of Native and Tissue-Engineered Articular Cartilage

Next generation tissue engineering of orthopedic soft tissue-to-bone interfaces

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