Chemiluminescence as a method for detection and study of free radicals in biological systems

Vladimirov, Yu. A.; Проскурнина, Е. В.; Izmailov, D. Yu.

doi:10.1007/s10517-007-0340-3

Cited by 22 publications

(14 citation statements)

References 11 publications

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“…The use of chemiluminescent probes amplifies chemiluminescence by allowing the detection of low levels of light emission, thereby increases the sensitivity of the reaction [27]. Antioxidants affect the intensity of luminol-dependent chemiluminescence [28].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Brazilian Plants Used to Treat Gastritis on the Oxidative Burst ofHelicobacter pylori-Stimulated Neutrophil

Bonacorsi

Fonseca

Raddi

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Ten Brazilian medicinal plants used to treat gastritis and ulcers were carefully selected on the basis of ethnopharmacological importance and antiulcerogenic activity previously described. The antioxidant activity of the methanolic extracts was determined in analysis conditions that simulate a real biological activity on inhibition of the oxidative burst induced in neutrophils using Helicobacter pylori as activator, by a luminol-amplified chemiluminescence assay. The extracts, at low concentration (5 μg/mL), exhibited a large variation in inhibitory effects of H. pylori-induced oxidative burst ranging from 48% inhibition to inactive, but all extracts, excluding Byrsonima intermedia, had inhibitory activity over 80% at the concentration of 100 μg/mL. The total suppressive antioxidant capacity measured as the effective concentration, which represents the extract concentration producing 50% inhibition of the chemiluminescence induced by H. pylori, varies from 27.2 to 56.8 μg/mL and was in the following order: Qualea parviflora > Qualea multiflora > Alchornea triplinervia > Qualea grandiflora > Anacardium humile > Davilla elliptica > Mouriri pusa > Byrsonima basiloba > Alchornea glandulosa > Byrsonima intermedia. The main groups of compounds in tested extracts are presented. Differences in the phytochemical profile, quantitatively and qualitatively, of these plants can explain and justify their protective effect on the gastric mucosa caused by the neutrophil-generated ROS that occurs when H. pylori displays its evasion mechanisms.

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Section: Introductionmentioning

confidence: 99%

Comparison of Brazilian Plants Used to Treat Gastritis on the Oxidative Burst ofHelicobacter pylori-Stimulated Neutrophil

Bonacorsi

Fonseca

Raddi

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…It is likely that not only high analytical power of LC-MS protocols in the identification of different individual signals, but also more integral approaches that afford on-line recording of the signaling process may be useful. Among them, a promising technique may be registration of different types of chemiluminescence originating from the excited states of oxidatively modified lipids [124]. Assuming that (phospho)lipid hydroperoxides are unavoidable primary molecular products of lipid peroxidation and that their decomposition gives rise to multiple secondary products with carbonyl functions in the excited state, it is likely that chemiluminescence protocols – particularly with physical enhancers of chemiluminescence responses – may be used for continuous monitoring of the peroxidation process in live cells.…”

Section: Discussionmentioning

confidence: 99%

Elimination of the unnecessary: Intra- and extracellular signaling by anionic phospholipids

Kagan

Bayır

Tyurina

et al. 2017

Biochemical and Biophysical Research Communications

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High fidelity of biological systems is frequently achieved by duplication of the essential intracellular machineries or, removal of the entire cell, which becomes unnecessary or even harmful in altered physiological environments. Carefully controlled removal of these cells, without damaging normal cells, requires precise signaling, and is critical to maintaining homeostasis. This review describes how two anionic phospholipids - phosphatidylserine (PS) and cardiolipin (CL) - residing in distinct compartments of the cell, signal removal of ”the unnecessary” using several uniform principles. One of these principles is realized by collapse of inherent transmembrane asymmetry and the externalization of the signal on the outer membrane surface - mitochondria for CL and the plasma membrane for PS – to trigger mitophagy and phagocytosis, respectively. Release from damaged cells of intracellular structures with externalized CL or externalized PS triggers their elimination by phagocytosis. Another of these principles is realized by oxidation of polyunsaturated species of CL and PS. Highly specific oxidation of CL by cytochrome c serves as a signal for mitochondria-dependent apoptosis, while oxidation of externalized PS improves its effectiveness to trigger phagocytosis of effete cells.

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“…Biochemiluminescence (luminescence observed during the synthesis of free radicals in biological systems) [38] Protection against diseases, against toxins…”

Section: H 2 O 2 )mentioning

confidence: 99%

To Age or Not to Age-Modern versus Traditional Concepts

2013

Altern Integ Med

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The modern oxidative hypothesis of ageing states that oxidative stress is a significant marker of senescence. This age related-oxidative stress is generated by a combination of increased free radicals production, decreased antioxidants levels and impaired repair of oxidative damages. Modern scientific anti-aging strategies delay the loss of physiological functions by modulating the activity of antioxidants and oxidants in the body. Various redox-dependent gerontomodulatory approaches include: (I) nutritional and pharmacological intervention by free radical scavengers (antioxidant therapy); (II) hormesis based interventions (caloric restriction, fasting, pro-oxidants). On the other hand, traditional Rasayana chikitsa (ayurvedic rejuvenation therapy) also provides certain dietary and therapeutic measures which are able to arrest process of ageing and can even rejuvenate whole functional dynamics of the body system. According to Ayurveda, reversal of ageing process is possible if Rasayana therapy is applied before a critical age (which depends upon the organ involved in sequential ageing process). Correlations between redox-dependent gerontomodulatory approaches and ayurvedic antiageing strategies (Rasayana chikitsa, preparatory procedures for Rasayana chikitsa) are presented and discussed in this paper. A literature search was conducted to collect data from studies on Rasayana drugs and fasting available up to November 2012 using PubMed and High wire.Based on this review, it appears that Rasayana drugs have cytoprotective and antioxidant effects, while fasting therapy has a direct effect on the modulation of free radicals synthesis. Thus, this integrative approach including modern and traditional concepts can be a lead towards future rejuvenative therapy research in the management of ageing.

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Chemiluminescence as a method for detection and study of free radicals in biological systems

Cited by 22 publications

References 11 publications

Comparison of Brazilian Plants Used to Treat Gastritis on the Oxidative Burst ofHelicobacter pylori-Stimulated Neutrophil

Comparison of Brazilian Plants Used to Treat Gastritis on the Oxidative Burst ofHelicobacter pylori-Stimulated Neutrophil

Elimination of the unnecessary: Intra- and extracellular signaling by anionic phospholipids

To Age or Not to Age-Modern versus Traditional Concepts

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