Chemiluminescence as an Analytical Tool in Cell Biology and Medicine

Campbell, Anthony K.; Hallett, Maurice Bartlett; Weeks, Ian

doi:10.1002/9780470110522.ch7

Cited by 75 publications

(8 citation statements)

References 471 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, changes in these three factors could potentially complicate the interpretation of bioluminescent images obtained with the ROSA26 ODD-Luc͞ϩ mice. Fortunately, the firefly luciferase K m for oxygen and ATP are Ϸ1-10 M and Ϸ50 M respectively (22,23,and A. Campbell,personal communication). In contrast, the oxygen K m for the HIF prolyl hydroxylases are Ϸ200 M, and cellular ATP levels are estimated to be Ϸ1 mM (24).…”

Section: Discussionmentioning

confidence: 99%

Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: Assessment of an oral agent that stimulates erythropoietin production

Safran

Kim

O’Connell

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

283

271

View full text Add to dashboard Cite

Many human diseases are characterized by the development of tissue hypoxia. Inadequate oxygenation can cause cellular dysfunction and death. Tissues use many strategies, including induction of angiogenesis and alterations in metabolism, to survive under hypoxic conditions. The heterodimeric transcription factor hypoxia-inducible factor (HIF) is a master regulator of genes that promote adaptation to hypoxia. HIF activity is linked to oxygen availability because members of the EGLN family hydroxylate HIF␣ subunits on specific prolyl residues when oxygen is present, which marks them for ubiquitination and proteasomal degradation. We created a mouse that ubiquitously expresses a bioluminescent reporter consisting of firefly luciferase fused to a region of HIF that is sufficient for oxygen-dependent degradation. Our validation studies suggest that this mouse will be useful for monitoring hypoxic tissues and evaluating therapeutic agents that stabilize HIF. One such agent, the HIF prolyl hydroxylase inhibitor FG-4383, was active in the liver and kidney after systemic administration as determined by bioluminescence imaging, transcription profiling, and production of erythropoietin, indicating that the HIF transcriptional program can be manipulated in vivo with orally active organic small molecules.bioluminescence ͉ imaging ͉ von Hippel-Lindau

show abstract

Section: Discussionmentioning

confidence: 99%

Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: Assessment of an oral agent that stimulates erythropoietin production

Safran

Kim

O’Connell

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

283

271

View full text Add to dashboard Cite

show abstract

“…The method offers advantages in sensitivity and reagent stability, detection precise control, and low biohazard [5]. Historically, luminol and isoluminol were the chemiluminescent labels of choice, but they became obsolete by the advantages of acridinium ester (AE) such as high inherent sensitivity, low quenching, simplicity and easy of handling and disposal, and a long shelflife [6]. Light from AE reaction is detected at 430 nm over a very short time frame, minimizing background noise and improving overall sensitivity exhibiting a linear response over an AE concentration range of more than 4 orders of magnitude, with a detection limit of approximately 5×10 −19 mol [7].…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescent Detection of Carbohydrates in the Tumoral Breast Diseases

Brustein¹,

Cavalcanti²,

Melo-Júnior³

et al. 2011

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Nowadays, there is an increase of investigations into the fibroadenoma, mainly because some studies have shown that the occurrence of fibroadenoma is linked to an increased risk of developing breast carcinoma. Currently, the chemiluminescence biomarkers are applied for validation methods and screening. Here, a lectin chemiluminescence is proposed as new histochemistry method to identify carbohydrates in mammary tumoral tissues. The lectins concanavalin A (Con A) and peanut agglutinin (PNA) conjugated to acridinium ester were used to characterize the glycocode of breast tissues: normal, fibroadenoma, and invasive duct carcinoma (IDC). The lectin chemiluminescence expressed in relative light units (RLU) was higher in fibroadenoma and IDC than in normal tissue for both lectins tested. The relationship RLU emission versus tissue area described a linear and hyperbolic curve for IDC and fibroadenoma, respectively, using Con A whereas hyperbolic curves for both transformed tissues using PNA. RLU was abolished by inhibiting the interaction between tissues and lectins using their specific carbohydrates: methyl-α-D: -mannoside (Con A) and galactose (PNA). The intrinsic fluorescence emission did not change with combination of the lectins (Con A/PNA) to the acridinium ester for hydrophobic residues. These results represent the lectin chemiluminescence as an alternative of histochemistry method for tumoral diagnosis in the breast.

show abstract

“…18,19 Recently, a Japanese manufacturer succeeded in applying CA to drug susceptibility testing. 20 In this study, we aimed to evaluate this measurement system in detection of glycopeptide resistance in enterococci.…”

Section: Introductionmentioning

confidence: 99%