Chemiluminescence on a Microchip

Wu, Xing-Zheng; Suzuki, Mari; Sawada, Tsuguo; Kitamori, Takehiko

doi:10.2116/analsci.16.321

Cited by 16 publications

(14 citation statements)

References 23 publications

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“…[1][2][3][4] The most commonly used detection mode in CE is UV absorption. More sensitive detection in CE can be achieved with laser-induced fluorescence, 5,6 electrochemical, 7,8 and chemiluminescence (CL) [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] approaches.…”

Section: Introductionmentioning

confidence: 99%

“…29 The coupling of CE-CL offers significantly high sensitivity and selectivity. [30][31][32] To date, a number of CL systems involving luminol, [10][11][12][13][14][15][16][17][18][19][20][21]28 peroxyoxalate, 9,10 permanganate, 24,27 ruthenium(II) complex, 25,26 acridinium esters 23 and firefly luciferase 15 have been utilized for the measurement of dyestuffs, proteins, amino acids, peptides, liposomes, alkaloids, phenols, ATP and metal ions with CE. Two main types of CL detection have been designed for CE.…”

Section: Introductionmentioning

confidence: 99%

“…31 Moreover, the possible mismatch between electroosmotic flow (EOF) and reagent stream made reproducible control of reagent concentration in the mixing capillary difficult. 33 The on-line mixing became much simpler in microchip CE-CL, [9][10][11] since crossings of different channel could be easily achieved in microchips.…”

Section: Introductionmentioning

confidence: 99%

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On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary

Zhang

2007

ANAL. SCI.

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On-capillary chemiluminescence detection for capillary electrophoresis with a single capillary was reported. A hole (about 30 μm diameter) was made on the capillary wall at about 50.5 cm from the inlet end. Hydrogen peroxide solution could enter the capillary from the hole, and mixed with luminol and copper(II) to produce chemiluminescence. The chemiluminescence was detected by a PMT under the hole. Several factors that influenced chemiluminescence intensity were investigated. The detection limits for luminol and N-(4-aminolbutyl)-N-ethylisoluminol (ABEI) were 1 × 10 -11 and 2 × 10 -10 mol L -1 , respectively. The method features simple construction and no dead volume.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary

Zhang

2007

ANAL. SCI.

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“…CL has been widely used in analytical chemistry for sensitive and selective analysis, especially over the last decade. Tsukagoshi et al proposed using CL in chemical analysis of the liquid-liquid interface of laminar flow in a microchannel; this technique is known as microchannel CL analysis (MCCLA) (9), (10), (11) .…”

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confidence: 99%

Diffusion in Microchannel Analyzed by Chemiluminescence

Matsumoto

Yoshida

Matsuo

2013

JTST

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We investigate diffusion and mixing in a microchannel by using luminol chemiluminescence (CL) to estimate the local chemical reaction rate. The degree of mixing in micromixers is generally evaluated from the deviation of concentration profiles measured by fluorescence from a uniform concentration profile. The degree of mixing measured by this method is a macroscopic estimate, which is inappropriate for investigating diffusion and mixing in microchannels. In this study, the luminol CL reaction is used to visualize and quantitatively measure local diffusion and mixing at an interface between two liquids in a microchannel. Blue CL is observed where luminol reacts with hydrogen peroxide at the mixing layer. Diffusion and mixing in a microchannel are investigated by sequentially measuring the CL and fluorescence. The experimental results are compared with the results of a numerical simulation that involves solving transport equations including the chemical reaction term. By calibrating the CL intensity with the chemical reaction rate estimated by the numerical simulation, the local chemical reaction can be quantitatively estimated from the CL intensity profile.

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“…4,5 Wu et al applied the luminol and peroxyoxalate CL methods to the detection of H2O2 in the integrated FIA system with gravity as a driving force. 6 In the FIA system, the reaction for detection is preferred to be complete within the residence time of the reactants in the flow cell. However, a fluid in a microfabricated channel is known to flow with laminar flow at a low flow rate.…”

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confidence: 99%

Effect of Mixing Modes on Chemiluminescent Detection of Epinephrine with Lucigenin by an FIA System Fabricated on a Microchip

et al. 2001

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The chemiluminescent (CL) detection of epinephrine (EP) with lucigenin (Luc) was performed using a micro flow cell fabricated on a silicon chip. A solution of EP was injected into the Luc carrier stream. The Luc solution containing EP and an alkaline solution were successively poured into the flow cell by a pressure-driven flow system. Two types of flow cells were fabricated for estimating the effect of the mixing modes in the flow cells on the intensity of light emission. In flow cell 1, two streams entered through separate inlet ports and merged to flow adjacently. In flow cell 2, a Luc solution containing EP was split up to 36 partial flows by passage through the nozzles, and was injected into the alkaline solution. The intensity of light emission in flow cell 2 increased markedly compared to that in flow cell 1. The detection limit of 8.0 × 10 -7 M for EP in flow cell 2 was a factor of six-times better than that in flow cell 1. The improvement in the sensitivity for EP could be explained in terms of the distortion of laminar flow in flow cell 2.

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Chemiluminescence on a Microchip

Cited by 16 publications

References 23 publications

On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary

On-capillary Chemiluminescence Detection for Capillary Electrophoresis with a Single Capillary

Diffusion in Microchannel Analyzed by Chemiluminescence

Effect of Mixing Modes on Chemiluminescent Detection of Epinephrine with Lucigenin by an FIA System Fabricated on a Microchip

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