ChemInform Abstract: A Pyruvated Carrageenan from Australian Specimens of the Red Alga Sarconema filiforme.

Chiovitti, Anthony; Bacic, Antony; Craik, David J.; Kraft, Gerald T.; Liao, Ming-Long; Falshaw, Ruth; Furneaux, R. H.

doi:10.1002/chin.199902226

Cited by 4 publications

(8 citation statements)

References 1 publication

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“…Signals at 25.79 and 101.7 were attributed to pyruvic methyl carbon and pyruvic acetal carbon respectively [28]. This pyruvylation of this galactan was reinforced by the signal at 65.88 ppm, specific of the C-6 and C-5 of a pyruvated ␤-d-galactopyranose unit [31][32][33].…”

Section: Nmr Spectroscopymentioning

confidence: 91%

“…No significative absorption bands around 850 and 805 cm −1 indicated the lack of axial sulphate ester substitutions at the O-4 of a 3-linked galactose and at the O-2 of a 4-linked 3,6-anhydrogalactose respectively[27]. In the same way, the very low intensity of absorption at 900 cm −1 suggested the polysaccharide contained minor ratio of unsulphated 3-linked galactopyranoses and/or 6-O methyl galactopyranose and/or unsulphated residues bearing pyruvate acetal at O-4 and O-6[28].…”

mentioning

confidence: 92%

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Highly sulphated galactan from Halymenia durvillei (Halymeniales, Rhodophyta), a red seaweed of Madagascar marine coasts

Fenoradosoa

Delattre²,

Laroche³

et al. 2009

International Journal of Biological Macromolecules

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Section: Nmr Spectroscopymentioning

confidence: 91%

mentioning

confidence: 92%

Highly sulphated galactan from Halymenia durvillei (Halymeniales, Rhodophyta), a red seaweed of Madagascar marine coasts

Fenoradosoa

Delattre²,

Laroche³

et al. 2009

International Journal of Biological Macromolecules

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“…The band at 816 cm −1 indicated that equatorial 6-sulfate groups on the galactosyl units had been formed [11]. The shoulder peak at 857 cm −1 suggested that the presence of axial sulfate ester at O-4 of some ␤-d-galactose residues [27,28]. In the spectra, the strong band at 1057 cm −1 was probably attributed to the symmetrical S O stretching vibration, and the band at 1128 cm −1 can be attributed to the asymmetrical C-O-S stretching vibration [29].…”

Section: Sulfation Of the Hydrolysis Derivatives Of Lacquer Polysacchmentioning

confidence: 99%

Preparation and in vitro antioxidant activity of lacquer polysaccharides with low molecular weights and their sulfated derivatives

Zou

et al. 2010

International Journal of Biological Macromolecules

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“…α-Carrageenan has been observed in the cell wall of several red algae such as Catenella nipae Zanardini (Zablackis and Santos, 1986 ), Sarconema filiforme (Sonder) Kylin (Chiovitti et al, 1998 ; Kumar et al, 2011 ) and in some Solieria spp. (Chiovitti et al, 1997 ; Bondu et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Discovery of a novel iota carrageenan sulfatase isolated from the marine bacterium Pseudoalteromonas carrageenovora

Genicot¹,

Groisillier²,

Rogniaux³

et al. 2014

Front. Chem.

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Carrageenans are sulfated polysaccharides extracted from the cell wall of some marine red algae. These polysaccharides are widely used as gelling, stabilizing, and viscosifying agents in the food and pharmaceutical industries. Since the rheological properties of these polysaccharides depend on their sulfate content, we screened several isolated marine bacteria for carrageenan specific sulfatase activity, in the aim of developing enzymatic bioconversion of carrageenans. As a result of the screening, an iota-carrageenan sulfatase was detected in the cell-free lysate of the marine bacterium Pseudoalteromonas carrageenovora strain PscT. It was purified through Phenyl Sepharose and Diethylaminoethyl Sepharose chromatography. The pure enzyme, Psc ι-CgsA, was characterized. It had a molecular weight of 115.9 kDaltons and exhibited an optimal activity/stability at pH ~8.3 and at 40 ± 5°C. It was inactivated by phenylmethylsulfonyl fluoride but not by ethylene diamine tetraacetic acid. Psc ι-CgsA specifically catalyzes the hydrolysis of the 4-S sulfate of iota-carrageenan. The purified enzyme could transform iota-carrageenan into hybrid iota-/alpha- or pure alpha-carrageenan under controlled conditions. The gene encoding Psc ι-CgsA, a protein of 1038 amino acids, was cloned into Escherichia coli, and the sequence analysis revealed that Psc ι-CgsA has more than 90% sequence identity with a putative uncharacterized protein Q3IKL4 from the marine strain Pseudoalteromonas haloplanktis TAC 125, but besides this did not share any homology to characterized sulfatases. Phylogenetic studies show that P. carrageenovora sulfatase thus represents the first characterized member of a new sulfatase family, with a C-terminal domain having strong similarity with the superfamily of amidohydrolases, highlighting the still unexplored diversity of marine polysaccharide modifying enzymes.

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ChemInform Abstract: A Pyruvated Carrageenan from Australian Specimens of the Red Alga Sarconema filiforme.

Cited by 4 publications

References 1 publication

Highly sulphated galactan from Halymenia durvillei (Halymeniales, Rhodophyta), a red seaweed of Madagascar marine coasts

Highly sulphated galactan from Halymenia durvillei (Halymeniales, Rhodophyta), a red seaweed of Madagascar marine coasts

Preparation and in vitro antioxidant activity of lacquer polysaccharides with low molecular weights and their sulfated derivatives

Discovery of a novel iota carrageenan sulfatase isolated from the marine bacterium Pseudoalteromonas carrageenovora

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