ChemInform Abstract: Enzymatic Methods of Analysis: Novel Approaches and Applications

Abstract

Analytical chemistry Z 0400 Enzymatic Methods of Analysis: Novel Approaches and Applications -[55 refs.]. -(SHEKHOVTSOVA*, T. N.; MUGINOVA, S. V.; VESELOVA, I. A.; Russ. Chem. Bull. 56 (2007) 4, 606-620; Dep. Chem., Lomonosov Moscow State Univ., Moscow 119992, Russia; Eng.) -Mischke 04-277

Application of Alkaline Phosphatases from Different Sources in Pharmaceutical and Clinical Analysis for the Determination of Their Cofactors; Zinc and Magnesium Ions

“…Three insulin dimers aggregate in the presence of zinc ions into a torus-shaped hexamer with two zinc-binding sites. Each binding site is comprised of three 10 HisB imidazole ligands with the corresponding 5 HisB residues in a close proximity (Fig. 4).…”

“…These procedures and aspects of their application in the analysis of real samples were discussed in details earlier. [8][9][10] In the present work, we focused on the application of intestinal alkaline phosphatases of animal origin in pharmaceutical and clinical analysis for the determination of zinc and magnesium ions, respectively. Alkaline phosphatase is a metalloenzyme.…”