ChemInform Abstract: MF‐EA‐705α and MF‐EA‐705β, New Metabolites from Microbial Fermentation of a Streptomyces sp.

Qureshi, Asfia; Mauger, Jacob B.; Cano, Raúl J.; Galazzo, Jorge L.; Lee, May D.

doi:10.1002/chin.200224223

Cited by 2 publications

(2 citation statements)

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“…The complete interpretation of data was in full agreement with the reported values of the compound NFAT-133 (Figure 3) (Burres et al 1995; Qureshi et al 2001). …”

Section: Resultssupporting

confidence: 88%

Fermentation, Isolation, Structure, and Antidiabetic Activity of NFAT-133 produced by Streptomyces strain PM0324667

et al. 2011

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Type-2 diabetes is mediated by defects in either insulin secretion or insulin action. In an effort to identify extracts that may stimulate glucose uptake, similar to insulin, a high throughput-screening assay for measuring glucose uptake in skeletal muscle cells was established. During the screening studies to discover novel antidiabetic compounds from microbial resources a Streptomyces strain PM0324667 (MTCC 5543, the Strain accession number at Institute of Microbial Technology, Chandigarh, India), an isolate from arid soil was identified which expressed a secondary metabolite that induced glucose uptake in L6 skeletal muscle cells. By employing bioactivity guided fractionation techniques, a tri-substituted simple aromatic compound with anti-diabetic potential was isolated. It was characterized based on MS and 2D NMR spectral data and identified as NFAT-133 which is a known immunosuppressive agent that inhibits NFAT-dependent transcription in vitro. Our investigations revealed the antidiabetic potential of NFAT-133. The compound induced glucose uptake in differentiated L6 myotubes with an EC50 of 6.3 ± 1.8 μM without activating the peroxisome proliferator-activated receptor-γ. Further, NFAT-133 was also efficacious in vivo in diabetic animals and reduced systemic glucose levels. Thus it is a potential lead compound which can be considered for development as a therapeutic for the treatment of type-2 diabetes. We have reported herewith the isolation of the producer microbe, fermentation, purification, in vitro, and in vivo antidiabetic activity of the compound.

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“…The complete interpretation of data was in full agreement with the reported values of the compound NFAT-133 (Figure 3) (Burres et al 1995; Qureshi et al 2001). …”

Section: Resultssupporting

confidence: 88%

Fermentation, Isolation, Structure, and Antidiabetic Activity of NFAT-133 produced by Streptomyces strain PM0324667

et al. 2011

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“…For S. rochei (10109), 2% glycerol was the best carbon source for optimal metabolite production. Glycerol is known to be an important medium component for the production of antimicrobial metabolites from microorganisms (Minambres et al 1992;Sengupta & Paul 1992;Qureshi et al 2001;Fukuda et al 2005;Fguira et al 2005;Mehdi et al 2006). Secondary metabolite production in actinomycetes is often stimulated by slowly assimilated complex carbohydrates in the production media, and decreased when more rapidly utilized monosaccharides such as glucose are present (Bertasso et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites

Reddy¹,

Ramakrishna²,

Rajagopal³

2011

Egypt. J. Bio.

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Fermentation and culture conditions were studied in shaken-flask culture to induce the production of greater amounts of antimicrobial metabolites by Streptomyces rochei (10109). Antimicrobial metabolite production started after 48 h incubation and reached its optimum level at 20% inoculum size at 120 h, at which point the metabolites showed maximum antifungal and antibacterial activity against selected human pathogenic microorganisms (Candida albicans, Staphylococcus aureus and Escherichia coli). Optimal production occurred at pH 7.5 and temperature 32°C, with 2% glycerol and 1% peptone as the carbon and nitrogen sources respectively. The effects of adding sea water (optimum 30%) and NaCl (optimum 1%) were also evaluated.

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ChemInform Abstract: MF‐EA‐705α and MF‐EA‐705β, New Metabolites from Microbial Fermentation of a Streptomyces sp.

Cited by 2 publications

References 1 publication

Fermentation, Isolation, Structure, and Antidiabetic Activity of NFAT-133 produced by Streptomyces strain PM0324667

Fermentation, Isolation, Structure, and Antidiabetic Activity of NFAT-133 produced by Streptomyces strain PM0324667

Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites

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