Chemometric analysis applied in 1H HR-MAS NMR and FT-IR data for chemotaxonomic distinction of intact lichen samples

Alcantara, Glaucia Braz; Honda, Nobuaki; Ferreira, Márcia M. C.; Ferreira, Antônio Gilberto

doi:10.1016/j.aca.2007.03.032

Cited by 33 publications

(25 citation statements)

References 25 publications

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“…Thus, elimination of these signals provides clearer spectra with less distortion at the baseline, making spectra more informative (Figure 1). A similar case in which this pulse sequence was used and good chemotaxonomic analysis results were achieved was reported by Alcantara et al 17 Average sample spectra for each H. speciosa variety (speciosa, cuyabensis, gardineri and pubescens) were generated and overlapped in an attempt to reveal the differences in the chemical composition (Figure 2a). Analysis of this figure reveals appreciable similarities between the four varieties, with differences observed only for var.…”

Section: Resultsmentioning

confidence: 71%

HR-MAS NMR Allied to Chemometric on Hancornia speciosa Varieties Differentiation

Flores¹,

Silva²,

Furquim³

et al. 2017

J. Braz. Chem. Soc.

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This work describes the potential of chemometric analyses applied to 1 H high-resolution magic angle spinning nuclear magnetic resonance ( 1 H HR-MAS NMR) data for the chemotaxonomic investigation of Hancornia speciosa (Apocynaceae) varieties. This plant, popularly known as mangaba, has a complex morphological differentiation and thus chemical analyses can be used for their taxonomic classification. In comparison to traditional techniques, 1 H HR-MAS NMR allied with chemometrics provided a simple and low cost method for chemotaxonomy. Leaves of four varieties of H. speciosa from a common garden experiment was studied and demonstrated that H. speciosa var. speciosa differs from others due to its specific metabolic profile, and var. pubescens was discriminated based on its high phenolic compound content. The distinction between the latter variety and gardineri is important once it allows for the selection of samples with greater commercial value, once they produce the largest and heaviest fruits.

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Section: Resultsmentioning

confidence: 71%

HR-MAS NMR Allied to Chemometric on Hancornia speciosa Varieties Differentiation

Flores¹,

Silva²,

Furquim³

et al. 2017

J. Braz. Chem. Soc.

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“…Já o grupo formado pela região de escores positivos para PC1 e negativos para PC2, composta pelos cultivares 2, 3 e 4, é rica em aminoácidos alifáticos (d 2,2 a 2,5 e d 3,0 a 3,2). Vale destacar que o feijão campeiro (8) é geneticamente descendente do feijão mulatinho (11), o que justifica a proximidade destes cultivares.…”

Section: Resultsunclassified

“…10 Na quimiotaxonomia de liquens, essa técnica demonstrou ser uma relevante ferramenta na discriminação entre famílias, gêneros e espécies, onde a identificação, baseada somente na análise morfológica, é comumente complexa e pouco conclusiva. 11 Vale destacar que a técnica HR-MAS é muito sensível e requer poucos minutos para o registro de sinais de RMN de 1 H utilizando alguns miligramas do analito. A técnica de RMN HR-MAS utiliza-se do fato de que os materiais, tanto vegetais quanto animais, quando no seu estado natural possuem moléculas de água em suas células na quantidade necessária para proporcionar mobilidade dos metabólitos (primários e secundários) e permitir a obtenção de espectros com excelente resolução, necessitando apenas da adição de uma ou duas gotas de solvente deuterado, adequadamente escolhido para não causar dissolução de parte dos constituintes das amostras -normalmente utiliza-se D 2 O para o ajuste da homogeneidade do campo magnético (shimming).…”

Section: Introductionunclassified

Perfil químico de cultivares de feijão (phaseolus vulgaris) pela técnica de high resolution magic angle spinning (HR-MAS)

Lião¹,

Choze²,

Cavalcante³

et al. 2010

Quím. Nova

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“…[10][11][12][13] NMR provides, in particular, the appropriate technology and sufficient resources to enable reproducible analysis for molecular identification and quantification. Moreover, NMR has been a technique of great relevance in studies concerning plant defense mechanisms through structural proteomics by allowing for structure determination and the elucidation of structure-function relationships.…”

Section: Introductionmentioning

confidence: 99%

1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis Caused by Xanthomonas axonopodis pv. citri

Silva¹,

Filho²,

Choze³

et al. 2012

J. Braz. Chem. Soc.

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A bactéria Xanthomonas axonopodis (Xac) causa uma das mais temidas e incuráveis doenças da citricultura: o cancro cítrico. Para compreender o mecanismo de resposta das laranjeiras quando atacadas pela Xac, folhas e frutos de Citrus sinensis foram diretamente avaliados por espectroscopia de RMN HRMAS (ressonância magnética nuclear de alta resolução com giro no ângulo mágico). Esta técnica permite a análise de amostras sem as laboriosas etapas de pré-tratamento, acessando as informações importantes sobre a sua composição química. As folhas de laranjeira e cascas do fruto investigadas neste estudo demonstraram as alterações bioquímicas causadas pela Xac. Auxiliado pelas análises quimiométricas, os resultados de RMN HRMAS apresentaram alterações relevantes no conteúdo de aminoácidos, carboidratos, ácidos orgânicos e terpenóides.Xanthomonas axonopodis (Xac) bacterium causes one of the most feared and untreatable diseases in citriculture: citrus canker. To understand the response mechanisms of orange trees when attacked by Xac, leaves and fruits of Citrus sinensis were directly evaluated by HRMAS NMR (high resolution magic angle spinning nuclear magnetic resonance) spectroscopy. This technique allows the analysis of samples without laborious pre-treatments and also allows access to important information about chemical composition of samples. The orange tree leaves and fruit peels investigated in this study demonstrated the biochemical changes caused by Xac. Aided by chemometric analysis, the HRMAS NMR results show relevant changes in amino acids, carbohydrates, organic acids and terpenoids content.

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Chemometric analysis applied in 1H HR-MAS NMR and FT-IR data for chemotaxonomic distinction of intact lichen samples

Cited by 33 publications

References 25 publications

HR-MAS NMR Allied to Chemometric on Hancornia speciosa Varieties Differentiation

HR-MAS NMR Allied to Chemometric on Hancornia speciosa Varieties Differentiation

Perfil químico de cultivares de feijão (phaseolus vulgaris) pela técnica de high resolution magic angle spinning (HR-MAS)

1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis Caused by Xanthomonas axonopodis pv. citri

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