Chemometric Strategies to Develop a Nanocomposite Electrode for Simultaneous Determination of Ascorbic Acid, Dopamine, and Uric Acid

Abstract

A simple and sensitive method for simultaneously measuring dopamine (DA), ascorbic acid (AA), and uric acid (UA) using a poly(1-aminoanthracene) and carbon nanotubes nanocomposite electrode is presented. The experimental parameters for composite film synthesis as well as the variables related to simultaneous determination of DA, AA, and UA were optimized at the same time using fractional factorial and Doehlert designs. The use of carbon nanotubes and poly(1-aminoanthracene) in association with a cathodic pretreatment led to three well-defined oxidation peaks at potentials around À0.039, 0.180 and 0.351 V (vs. Ag/AgCl) for AA, DA, and UA, respectively. Using differential pulse voltammetry, calibration curves for AA, DA, and UA were obtained over the range of 0.16-3.12 10 À3 mol L , respectively. The proposed method was successfully applied to determine DA, AA, and UA in biological samples with good results.

PEDOT-modified integrated microelectrodes for the detection of ascorbic acid, dopamine and uric acid

“…To overcome this problem, many modification strategies have been adopted to lower the overpotential, to increase detection sensitivity and to improve selectivity. In the frame of antioxidant detection, they have led to the realization of various modified (micro)electrodes based on quantum dots [24], nanoparticles [25][26][27], carbon nanotubes [28][29][30], graphene [25][26][27]29,[31][32][33] and conductive polymers [28,[34][35][36]. Among them, poly(3,4-ethylenedioxythiophene) (PEDOT) was one of the widely used conducting polymers for the detection of AA, Dop and UA [37][38][39][40][41][42].…”

Synthesis of Polyfluorinated Azobenzene Intercalated Tantalum Tungstate Nanocomposite for Determination of Dopamine and Ascorbic Acid

“…It can be concluded that the C 3 F 7 -azo + -TaWO 6 /GCE has excellent ability to distinguish between DA and AA and has a lower detection limit of DA and AA. [44][45][46][47][48][49][50] The stability and reproducibility of C 3 F 7 -azo + -TaWO 6 /GCE were analyzed using DPV technology. All the above experiments were performed in N 2 -saturated PBS (0.1 M, pH = 6) containing AA (0.2 mM) and DA (0.2 mM).…”

Recent Advances in Electroanalysis of Vitamins

“…Electrochemical methods for its determination were recently reviewed , nevertheless many others were proposed since then . Many of these methods allow the determination of ascorbic acid in the presence of several other analytes, more commonly uric acid and dopamine since they are co‐existing in biological matrixes and they are oxidized at almost the same potential at traditional electrode materials. Other methods were demonstrated successfully for mixture of ascorbic acid with some drugs , especially acetaminophen or components regulating the physiological function of organisms .…”