Chemotherapeutics-induced Oct4 expression contributes to drug resistance and tumor recurrence in bladder cancer

Lu, Chia Sing; Shieh, Gia Shing; Wang, Chung Teng; Su, Bing; Su, Yu; Chen, Yi Cheng; Su, Wu Chou; Wu, Pensée; Yang, Wen-Horng; Shiau, Ai‐Li; Wu, Chao

doi:10.18632/oncotarget.9602

Cited by 50 publications

(48 citation statements)

References 53 publications

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“…5 In addition, chemotherapeutics can induce multidrug resistance, leading to treatment failure upon recurrence of the disease. 6,7 Some plant extracts have shown interesting anticancer properties without the serious side effects of cytotoxic agents. Among them, curcumin (CUR), which has anti-inflammatory activities, has also shown activity against cancer through multiple mechanisms, including inhibition of initiation, progression, invasion and metastasis of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Liposomal curcumin and its application in cancer

Feng¹,

Wei²,

Lee³

et al. 2017

IJN

314

208

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Curcumin (CUR) is a yellow polyphenolic compound derived from the plant turmeric. It is widely used to treat many types of diseases, including cancers such as those of lung, cervices, prostate, breast, bone and liver. However, its effectiveness has been limited due to poor aqueous solubility, low bioavailability and rapid metabolism and systemic elimination. To solve these problems, researchers have tried to explore novel drug delivery systems such as liposomes, solid dispersion, microemulsion, micelles, nanogels and dendrimers. Among these, liposomes have been the most extensively studied. Liposomal CUR formulation has greater growth inhibitory and pro-apoptotic effects on cancer cells. This review mainly focuses on the preparation of liposomes containing CUR and its use in cancer therapy.

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Section: Introductionmentioning

confidence: 99%

Liposomal curcumin and its application in cancer

Feng¹,

Wei²,

Lee³

et al. 2017

IJN

314

208

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“…These result further indicate the importance of OCT4 contributing to the development of cancers in a number of ways (18,19). The aim of the present study was to investigate the role of miR-145 in the modulation of cervical cancer cell radiosensitivity, using transfection with miR-145 mimics to upregulate miR-145 in cervical cancer tera cells.…”

Section: Microrna-145 Sensitizes Cervical Cancer Cells To Low-dose Irmentioning

confidence: 91%

MicroRNA-145 sensitizes cervical cancer cells to low-dose irradiation by downregulating OCT4 expression

Yan

Jin

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Poor elucidation of the mechanisms involved in regulating the radiosensitivity of cancers prevents the extensive application of low-dose radiotherapy in clinical settings. The present study was conducted to investigate the role of microRNA-145 (miR-145) in the modulation of cervical cancer cell radiosensitivity, as well as to identify the underlying target of miR-145 during this process. Cervical cancer tera cells were initially exposed to doses of radiation between 1 and 6 Gy before the assessments of the cell viability and apoptosis rate. Irradiation at dose of 1 Gy was screened as optimum dose and used in subsequent experiments. A dual luciferase reporter assay was performed to demonstrate that octamer-binding transcription factor 4 (OCT4) is a target of miR-145 in cervical cancer. Consequently, OCT4 was suggested to be a target of miR-145, as a dual luciferase vector that was ligated to a fragment corresponding to the predicted target site of miR-145 in OCT4 3'-UTR showed an 83% reduction in fluorescence. Following exposure to 1 Gy irradiation, tera cells transfected with miR-145 mimics, which showed downregulation of OCT4 and cyclin D1, had lower cell viability and cell migration rate and higher apoptosis rate compared to non-transfected cells. However, the co-transfection of miR-145 mimics and OCT4 expression vector restored OCT4 and cyclin D1 expression levels and made no significant difference in terms of cell viability, cell migration rate and apoptosis rate. The present results indicate that miR-145 increases the radiosensitivity of cervical cancer cells by silencing OCT4, that cyclin D1 is putatively under the positive regulation of OCT4 and mediates miR-145 function.

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“…The results indicated that both HepG2 and Huh7 cells expressed lower levels of EpCAM when cultured in a 2D manner, whereas EpCAM expression was significantly higher in cells cultured on cellulosic scaffolds ( Figure 4B). 26,27 Oct4 expression was examined in HCC cells grown in the 2D system and on the cellulosic scaffolds by Western blot analysis. 26,27 Oct4 expression was examined in HCC cells grown in the 2D system and on the cellulosic scaffolds by Western blot analysis.…”

Section: Hcc Cell Spheroids Show Resistance To Chemotherapy In Vitromentioning

confidence: 99%

Microporous cellulosic scaffold as a spheroid culture system modulates chemotherapeutic responses and stemness in hepatocellular carcinoma

Zhan

Chen³

et al. 2018

J of Cellular Biochemistry

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Hepatocellular carcinoma (HCC) treatments are evaluated by two‐dimensional (2D) in vitro culture systems, despite their limited ability to predict drug efficacy. The three‐dimensional (3D) microporous scaffold provides the possibility of generating more reliable preclinical models to increase the efficacy of cancer treatments. The physical properties of a microporous cellulosic scaffold were evaluated. The cellulosic scaffold was biocompatible and had a highly porous network with appropriate pore size, swelling rate, and stiffness of cancer cell cultures. Cellulosic scaffolds were compared with 2D polystyrene for the culture of HepG2 and Huh7 human HCC cells. Cellulosic scaffolds promoted tumor spheroid formation. Cells cultured on scaffolds were more resistant to chemotherapy drugs and showed upregulation of EpCAM and Oct4. The migration ability of HCC cells cultured on scaffolds was significantly greater than that of cells grown in 2D cultures as evidenced by the downregulation of E‐cadherin. In addition, the proportion of CD44+/CD133+ HCC cancer stem cells (CSCs) was significantly greater in cells cultured on scaffolds than in those grown in 2D cultures. These findings suggest that cellulosic scaffolds effectively mimic the in vivo tumor behavior and may serve as a platform for the study of anticancer therapeutics and liver CSCs.

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Chemotherapeutics-induced Oct4 expression contributes to drug resistance and tumor recurrence in bladder cancer

Cited by 50 publications

References 53 publications

Liposomal curcumin and its application in cancer

Liposomal curcumin and its application in cancer

MicroRNA-145 sensitizes cervical cancer cells to low-dose irradiation by downregulating OCT4 expression

Microporous cellulosic scaffold as a spheroid culture system modulates chemotherapeutic responses and stemness in hepatocellular carcinoma

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