Chicken CD56 defines NK cell subsets in embryonic spleen and lung

Neulen, Marie Luise; Göbel, Thomas

doi:10.1016/j.dci.2012.08.001

Cited by 13 publications

(5 citation statements)

References 36 publications

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“…NK cell subsets in mice and humans are clustered based on the expression of CD56 and CD16 on their surface. Avian NK cells also express CD56 molecules (Neulen & Göbel, 2012). The extent of expression of a complex array of surface molecules determines the specific localization and function of NK cells (Cooper et al, 2001).…”

Section: Natural Killer (Nk) Cellsmentioning

confidence: 99%

Development of innate immunity in chicken embryos and newly hatched chicks: a disease control perspective

et al. 2019

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Newly hatched chickens are confronted by a wide array of pathogenic microbes because their adaptive immune defences have limited capabilities to control these pathogens. In such circumstances, and within this age group, innate responses provide a degree of protection. Moreover, as the adaptive immune system is relatively naïve to foreign antigens, synergy with innate defences is critical. This review presents knowledge on the ontogeny of innate immunity in chickens pre-hatch and early post-hatch and provides insights into possible interventions to modulate innate responses early in the life of the bird. As in other vertebrate species, the chicken innate immune system which include cellular mediators, cytokine and chemokine repertoires and molecules involved in antigen detection, develop early in life. Comparison of innate immune systems in newly hatched chickens and mature birds has revealed differences in magnitude and quality, but responses in younger chickens can be boosted using innate immune system modulators. Functional expression of pattern recognition receptors and several defence molecules by innate immune system cells of embryos and newly hatched chicks suggests that innate responses can be modulated at this stage of development to combat pathogens. Improved understanding of innate immune system ontogeny and functionality in chickens is critical for the implementation of sound and safe interventions to provide long-term protection against pathogens. Next-generation tools for studying genetic and epigenetic regulation of genes, functional metagenomics and gene knockouts can be used in the future to explore and dissect the contributions of signalling pathways of innate immunity and to devise more efficacious disease control strategies.

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Section: Natural Killer (Nk) Cellsmentioning

confidence: 99%

Development of innate immunity in chicken embryos and newly hatched chicks: a disease control perspective

et al. 2019

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“…Proportions of NK cells were analysed by flow cytometry using markers that are known to be expressed on chicken NK cells; the chicken homologue of the human NK-cell marker CD5658 and the previously described NK-cell markers 20E5, 7C138 and 28-439. Since some of the NK-cell markers are also expressed on T cells38, staining with NK-cell markers was combined with anti-CD3 mAb.…”

Section: Methodsmentioning

confidence: 99%

Differential lung NK cell responses in avian influenza virus infected chickens correlate with pathogenicity

Jansen

Geus

Haarlem

et al. 2013

Sci Rep

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Infection of chickens with low pathogenicity avian influenza (LPAI) virus results in mild clinical signs while infection with highly pathogenic avian influenza (HPAI) viruses causes death of the birds within 36–48 hours. Since natural killer (NK) cells have been shown to play an important role in influenza-specific immunity, we hypothesise that NK cells are involved in this difference in pathogenicity. To investigate this, the role of chicken NK-cells in LPAI virus infection was studied. Next activation of lung NK cells upon HPAI virus infection was analysed. Infection with a H9N2 LPAI virus resulted in the presence of viral RNA in the lungs which coincided with enhanced activation of lung NK cells. The presence of H5N1 viruses, measured by detection of viral RNA, did not induce activation of lung NK cells. This suggests that decreased NK-cell activation may be one of the mechanisms associated with the enhanced pathogenicity of H5N1 viruses.

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“…Expression of receptors also varies between tissues and subpopulations of NK cells in the lung, liver, and intestinal epithelium ( 52 ). These include antibodies specific for CD56 ( 49 ) and CHIR-AB1 ( 53 ), a high-affinity IgY Fc receptor. Nonetheless, they are useful for flow cytometry in multicolour panels in combination with the lack of surface CD3, BAFF-R, or Ig Light chain staining ( 48 ).…”

Section: Natural Killer Cellsmentioning

confidence: 99%

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

Härtle,

Sutton,

Vervelde

et al. 2024

Front. Vet. Sci.

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Multiparameter flow cytometry is a routine method in immunological studies incorporated in biomedical, veterinary, agricultural, and wildlife research and routinely used in veterinary clinical laboratories. Its use in the diagnostics of poultry diseases is still limited, but due to the continuous expansion of reagents and cost reductions, this may change in the near future. Although the structure and function of the avian immune system show commonalities with mammals, at the molecular level, there is often low homology across species. The cross-reactivity of mammalian immunological reagents is therefore low, but nevertheless, the list of reagents to study chicken immune cells is increasing. Recent improvement in multicolor antibody panels for chicken cells has resulted in more detailed analysis by flow cytometry and has allowed the discovery of novel leukocyte cell subpopulations. In this article, we present an overview of the reagents and guidance needed to perform multicolor flow cytometry using chicken samples and common pitfalls to avoid.

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Chicken CD56 defines NK cell subsets in embryonic spleen and lung

Cited by 13 publications

References 36 publications

Development of innate immunity in chicken embryos and newly hatched chicks: a disease control perspective

Development of innate immunity in chicken embryos and newly hatched chicks: a disease control perspective

Differential lung NK cell responses in avian influenza virus infected chickens correlate with pathogenicity

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

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