Chill coma temperatures appear similar along a latitudinal gradient, in contrast to divergent chill coma recovery times, in two widespread ant species

Maysov, Andrey

doi:10.1242/jeb.096958

Cited by 7 publications

(10 citation statements)

References 34 publications

(41 reference statements)

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“…Latitudinal variation in CCRT, but not in CT min , has been found e.g. in two widespread ant species, Myrmica rubra and Myrmica ruginodis [29]. Our study gave some support to this hypothesis, as CCRT showed significant variation in both species, while CT min showed it only in D. montana .…”

Section: Discussionsupporting

confidence: 79%

“…Numerous studies have evidenced inter- and intraspecific latitudinal variation in insect cold tolerance (e.g. [1, 2, 30, 3–6, 18, 27–29]) and identified candidate genes for it (e.g. [61–63, 65]).…”

Section: Discussionmentioning

confidence: 99%

“…Latitudinal variation in cold tolerance has been detected in a variety of species, including Drosophila flies [18, 27, 28], Myrmica ants [29], Porcellio laevis woodlouse [30] and Arabidopsis thaliana plant [31, 32]. However, it is challenging to distinguish whether such variation has evolved in response to changes in photoperiod (day length), temperature, or their combination [7].…”

Section: Introductionmentioning

confidence: 99%

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Multiple paths to cold tolerance: the role of environmental cues, morphological traits and the circadian clock genevrille

Poikela

Tyukmaeva

Hoikkala

et al. 2020

Preprint

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20Identifying elements that enhance insect adaptation into changing environmental conditions is 21 challenging, especially for traits affected by multiple factors. We studied latitudinal variation 22 in the basal cold tolerance and body colour of two highly cold-tolerant Drosophila virilis group 23 species, D. montana and D. flavomontana, in climatically diverse locations in North America. 24 D. montana, which is generally found on higher latitudes and altitudes than D. flavomontana, 25 was darker and more cold-tolerant than D. flavomontana. In D. montana only fly cold tolerance 26 showed latitudinal variation, while in D. flavomontana both traits varied according to latitude 27 and local climatic conditions, but showed no correlation with each other. We also examined the 28 role of a circadian clock gene vrille and an insulin-signalling pathway gene Yolk protein 3 (Yp3) 29 in the basal cold tolerance and cold acclimation ability of D. montana females using RNA 30 interference. Silencing of vrille induced expression changes in the period, but not clock, and 31 decreased flies' basal cold tolerance and cold acclimation ability, while silencing of Yp3 32 affected only cold acclimation. Our study demonstrates that the dependence of insect cold 33 tolerance on latitudinally varying factors and local climatic conditions may vary even between 34 closely-related species. Furthermore, we propose that a functional circadian clock system plays 35 an essential role both in insect basal cold tolerance and cold acclimation ability, and that Yp3 36 affects cold acclimation likely through its interactions with other genes in the insulin-signalling 37 pathway. 38 39 40

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple paths to cold tolerance: the role of environmental cues, morphological traits and the circadian clock genevrille

Poikela

Tyukmaeva

Hoikkala

et al. 2020

Preprint

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“…These characteristics make CCRT a good indicator of climatic adaptation. Latitudinal variation in CCRT, but not in CT min , has also been observed in two widespread ant species, Myrmica rubra and Myrmica ruginodis [ 29 ]. Although it is possible that the low variation in cold tolerance in our study is a consequence of relatively low number of studied strains or populations, a recent study found similar trends when using mass-bred D. montana populations for a limited number of places from North America [ 89 ].…”

Section: Discussionmentioning

confidence: 99%

“…Latitudinal clines in cold tolerance traits, detected in a variety of species, including Drosophila flies [ 16 , 27 , 28 ], Myrmica ants [ 29 ], Porcellio laevis woodlouse [ 30 ], highlight adaptive genetic variation in these traits. However, it is challenging to distinguish whether such variation has evolved in response to changes in photoperiod (day length), temperature or their combination [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Multiple paths to cold tolerance: the role of environmental cues, morphological traits and the circadian clock gene vrille

et al. 2021

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Background Tracing the association between insect cold tolerance and latitudinally and locally varying environmental conditions, as well as key morphological traits and molecular mechanisms, is essential for understanding the processes involved in adaptation. We explored these issues in two closely-related species, Drosophila montana and Drosophila flavomontana, originating from diverse climatic locations across several latitudes on the coastal and mountainous regions of North America. We also investigated the association between sequence variation in one of the key circadian clock genes, vrille, and cold tolerance in both species. Finally, we studied the impact of vrille on fly cold tolerance and cold acclimation ability by silencing it with RNA interference in D. montana. Results We performed a principal component analysis (PCA) on variables representing bioclimatic conditions on the study sites and used latitude as a proxy of photoperiod. PC1 separated the mountainous continental sites from the coastal ones based on temperature variability and precipitation, while PC2 arranged the sites based on summer and annual mean temperatures. Cold tolerance tests showed D. montana to be more cold-tolerant than D. flavomontana and chill coma resistance (CTmin) of this species showed an association with PC2. Chill coma recovery time (CCRT) of both species improved towards northern latitudes, and in D. flavomontana this trait was also associated with PC1. D. flavomontana flies were darkest in the coast and in the northern mountainous populations, but coloration showed no linkage with cold tolerance. Body size decreased towards cold environments in both species, but only within D. montana populations largest flies showed fastest recovery from cold. Finally, both the sequence analysis and RNAi study on vrille suggested this gene to play an essential role in D. montana cold resistance and acclimation, but not in recovery time. Conclusions Our study demonstrates the complexity of insect cold tolerance and emphasizes the need to trace its association with multiple environmental variables and morphological traits to identify potential agents of natural selection. It also shows that a circadian clock gene vrille is essential both for short- and long-term cold acclimation, potentially elucidating the connection between circadian clock system and cold tolerance.

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Chill coma recovery of Ceratitis capitata adults across the Northern Hemisphere

Moraiti

Verykouki

Papadopoulos

2022

Sci Rep

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The Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae), is an invasive pest, that is currently expanding its geographic distribution from the Mediterranean coasts to more temperate areas of Europe. Given that low temperature is a primary determinant of insect species’ range boundaries especially in the Northern Hemisphere with pronounced seasonality, we used chill coma recovery time for assessing latitudinal clines in basal chill tolerance of C. capitata adults. We selected six populations obtained from areas with broad climatic variability based on the main bioclimatic variables of temperature and precipitation, spanning a latitudinal range of about 19° from Middle East to Central Europe. Adults were exposed to 0 °C for 4 h, and time to regain the typical standing position of a fly at 25 °C were recorded. The post-stress survival after a period of 8 days was also recorded. Results revealed that adults from Israel and Austria were less chill tolerant than those from Greece, resulting in curvilinear trends with latitude. Analysis of macroclimatic conditions revealed combined effects of latitude (as a proxy of photoperiod) and macroclimatic conditions on chill coma recovery time. Nonetheless, there was not a deleterious effect on post-recovery survival, except for flies obtained from the northern most point (Vienna, Austria). Overall, it seems that evolutionary patterns of basal chill coma recovery time of C. capitata adults are driven mainly by local climatic variability.

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Chill coma temperatures appear similar along a latitudinal gradient, in contrast to divergent chill coma recovery times, in two widespread ant species

Cited by 7 publications

References 34 publications

Multiple paths to cold tolerance: the role of environmental cues, morphological traits and the circadian clock genevrille

Multiple paths to cold tolerance: the role of environmental cues, morphological traits and the circadian clock genevrille

Multiple paths to cold tolerance: the role of environmental cues, morphological traits and the circadian clock gene vrille

Chill coma recovery of Ceratitis capitata adults across the Northern Hemisphere

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