Chilling-stress modifies DNA methylation level in cucumber (Cucumis sativus L.) seedling radicle to regulate elongation rate

Chen, Bixuan; Saltveit, Mikal E.; Beckles, Diane M.

doi:10.1016/j.scienta.2019.03.023

Cited by 23 publications

(14 citation statements)

References 38 publications

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“…However, not all sites methylated due to chilling were reversible after rewarming, and explains why '5T' fruit were of poorer quality than other Turning fruit. This is in agreement with the growth rate and methylation pattern of chilled and rewarmed cucumber radicles (Chen et al, 2019).…”

Section: Discussionsupporting

confidence: 89%

“…Alteration in DNA methylation of tomato fruit was determined by comparing the site type (see Fig. 1B and Table S4) from two different treatments, and the definition of "de novo methylation", "demethylation" and "no change" was according to Chen et al, (2019). Table (adapted) (Fulneček & Kovařík, 2014;Guarino et al, 2019).…”

Section: Methyl-sensitive Amplification Polymorphism (Msap)mentioning

confidence: 99%

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Postharvest handling induces changes in fruit DNA methylation status and is associated with alterations in fruit quality in tomato (Solanum lycopersicumL.)

Zhou

Chen

Albornoz

et al. 2020

Preprint

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Postharvest handling of tomato (Solanum lycopersicum L.), specifically low-temperature storage and early harvest are used to extend shelf life, but often reduce fruit quality. Recent work suggests that DNA methylation dynamics influences fruit ripening through the demethylase SlDML2 gene. However, the influence of postharvest handling on DNA methylation in relation to fruit quality is unclear. This work aimed to clarify these issues by analyzing DNA methylation using methyl-sensitive amplification polymorphism (MSAP), semi-quantitative transcriptional analysis of marker genes for fruit quality (RIN; RIPENING INHIBITOR) and DNA methylation (SlDML2; Solanum lycopersicum L. DNA demethylase 2), and, fruit biochemical quality biomarkers. Multivariate analysis of these data supported the view that DNA methylation of fruit was influenced more by postharvest handling than ripening stage, however, fruit quality was influenced mainly by ripening. Fruit chilled postharvest were distinct in their DNA methylation state and quality characteristics, which implied that these three phenomena i.e., chilling, methylation, and quality are highly connected. In addition, different postharvest handling methods modulated SlDML2 transcript levels but had little effect on the level of RIN transcripts in fruit that reached the Turning stage after early harvest, and cold storage. These data collectively helped to advance our interpretation of tomato fruit ripening. In conclusion, our findings revealed that postharvest-induced variation in fruit quality is in relation to DNA methylation. Long-term this work will help better connect physiological changes in tomato fruit to events happening at the molecular level.

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Section: Discussionsupporting

confidence: 89%

Section: Methyl-sensitive Amplification Polymorphism (Msap)mentioning

confidence: 99%

Postharvest handling induces changes in fruit DNA methylation status and is associated with alterations in fruit quality in tomato (Solanum lycopersicumL.)

Zhou

Chen

Albornoz

et al. 2020

Preprint

Self Cite

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“…Cucumber (Cucumis sativus L.) is an economically important Cucurbit vegetable that is widely cultivated worldwide. Global production of cucumber reached 80 million metric tons in 2016 [33]. During production, cucumber often suffers from various abiotic stresses, such as low temperatures, high temperatures, and salinity.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Characterization of Cucumber (Cucumis sativus L.) GRAS Genes and Their Response to Various Abiotic Stresses

Dong

et al. 2020

Horticulturae

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The GRAS (gibberellic acid insensitive, repressor of GAI, and scarecrow) proteins are a family of plant-specific transcription factors that regulate plant growth, development, and stress response. Currently, the role of GRAS transcription factors in various abiotic stress responses has not been systematically studied in cucumber (Cucumis sativus L.), a popular vegetable crop. Here, we provide a comprehensive bioinformatics analysis of the 35 GRAS genes identified in the cucumber genome. In this study, cucumber genotypes, i.e., “CG104”, which is stress-tolerant, and genotype “CG37”, which is stress-sensitive, were examined to provide insight on potential differences in the GRAS-regulated abiotic stress pathways. Transcriptional analysis by RNA-seq or qRT-PCR of these two genotypes revealed common and divergent functions of CsGRAS genes regulated by low and high temperatures, salinity, and by exposure to the phytohormones gibberellin (GA) and abscisic acid (ABA). Notably, CsGRAS2 (DELLA) and CsGRAS26 (LISCL) were regulated by all abiotic stresses and hormone treatments, suggesting that they may function in the biological cross-talk between multiple signaling pathways. This study provides candidate genes for improving cucumber tolerance to various environmental stresses.

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“…inTrODUCTiOn Cucumber (Cucumis sativus L.) is an economically important vegetable crop. Global production reached 80 million metric tons in 2016, with a steady increase in production since 1998 (Chen et al, 2019). Cucumber originated from tropical regions, and the suitable temperature range for growth is 18-30°C.…”

mentioning

confidence: 99%

Quantitative Trait Loci Mapping and Candidate Gene Analysis of Low Temperature Tolerance in Cucumber Seedlings

Dong

Wang

et al. 2019

Front. Plant Sci.

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Cucumber (Cucumis sativus L.) is an economically important vegetable crop worldwide, but it is sensitive to low temperatures. Cucumber seedlings exposed to long-term low temperature stress (LT), i.e., below 20°C during the day, and 8°C at night, exhibit leaf yellowing, accelerated senescence, and reduced yield, therefore posing a threat to cucumber production. Studying the underlying mechanisms involved in LT tolerance in cucumber seedlings, and developing germplasm with improved LT-tolerance could provide fundamental solutions to the problem. In this study, an F 2 population was generated from two parental lines, "CG104" (LT-tolerant inbred line) and "CG37" (LT-sensitive inbred line), to identify loci that are responsible for LT tolerance in cucumber seedlings. Replicated phenotypic analysis of the F 2-derived F 3 family using a low-temperature injury index (LTII) suggested that the LT tolerance of cucumber seedlings is controlled by multiple genes. A genetic map of 990.8 cM was constructed, with an average interval between markers of 5.2 cM. One quantitative trait loci (QTL) named qLTT5.1 on chromosome 5, and two QTLs named qLTT6.1 and qLTT6.2 on chromosome 6 were detected. Among them, qLTT6.2 accounted for 26.8 and 24.1% of the phenotypic variation in two different experiments. Single-nucleotide polymorphism (SNP) variations within the region of qLTT6.2 were analyzed using two contrasting in silico bulks generated from the cucumber core germplasm. Result showed that 214 SNPs were distributed within the 42-kb interval, containing three candidate genes. Real-time quantitative reverse transcription PCR and sequence analysis suggested that two genes Csa6G445210, an auxin response factor, and Csa6G445230, an ethylene-responsive transmembrane protein, might be candidate genes responsible for LT tolerance in cucumber seedlings. This study furthers the understanding of the molecular mechanism underlying LT tolerance in cucumber seedlings, and provides new markers for molecular breeding.

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Chilling-stress modifies DNA methylation level in cucumber (Cucumis sativus L.) seedling radicle to regulate elongation rate

Cited by 23 publications

References 38 publications

Postharvest handling induces changes in fruit DNA methylation status and is associated with alterations in fruit quality in tomato (Solanum lycopersicumL.)

Postharvest handling induces changes in fruit DNA methylation status and is associated with alterations in fruit quality in tomato (Solanum lycopersicumL.)

Genome-Wide Characterization of Cucumber (Cucumis sativus L.) GRAS Genes and Their Response to Various Abiotic Stresses

Quantitative Trait Loci Mapping and Candidate Gene Analysis of Low Temperature Tolerance in Cucumber Seedlings

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