Chimeric hexon HVRs protein reflects partial function of adenovirus

Yu, Bin; Wang, Chu; Dong, Jianing; Zhang, Min; Zhang, Haihong; Wu, Jiaxin; Wu, Yuqing; Kong, Wei; Yu, Xianghui

doi:10.1016/j.bbrc.2012.03.125

Cited by 18 publications

(21 citation statements)

References 17 publications

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“…Because of its specific nature and structure (with regions that are strictly conserved and hypervariable regions located in Loop L1 (HVR1-4), the hexon gene is often a target of research on taxonomy and antigenic properties of FAdVs [3,12,17,21]. Conserved sequences that are very similar or almost identical in every FAdV serotype cannot be used for strain discrimination [3,12,17,18].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Niczyporuk

2015

Arch Virol

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Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbreaks and developing strategies for vaccine development. Several molecular epidemiology and phylogenetic studies have been performed, but the results obtained are still limited, because FAdV strains, even of the same serotype, have very diverse characteristics. Some strains are pathogenic and some are nonpathogenic. This report describes the successful isolation of 96 FAdV field strains from chickens in Poland. A PCR assay specific for the L1 loop region of the hexon gene was conducted, and the products were subjected to sequence analysis. The sequences were analysed using BLAST and Geneious 6.0 software and compared to adenovirus field and reference strain sequences from different parts of the world that are accessible in the NCBI GenBank database. The sequences of the adenovirus strains indicated that they belonged to five species, Fowl aviadenovirus A-E, represented by eight serotypes FAdV-1, FAdV-4, FAdV-5, FAdV-7, FAdV-8a, FAdV-8b, and FAdV-2/11 (FAdV-D). The relationships between FAdVs isolated in Poland and isolates from other regions of the world were determined.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Niczyporuk

2015

Arch Virol

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“…In most cases, researchers target hypervariable regions and HVR1-4 flanking the conserved regions for studies on the taxonomy and antigenic properties of adenoviruses [10, 16, 21, 23–26, 31]. The potential effects of mutations on the hexon protein structure were examined.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterisation of fowl adenovirus type 7 isolated from poultry associated with inclusion body hepatitis in Poland

Niczyporuk

2017

Arch Virol

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The fowl adenovirus field strain FAdV-JSN-5/10j (GenBank accession number KP879219) was isolated from the intestine of a 7-week-old chicken diagnosed with inclusion body hepatitis and simultaneously with Marek’s disease, and for that reason, it was chosen for molecular study. It was identified as fowl adenovirus genotype 7 (species Fowl aviadenovirus E) based on nucleotide sequence analysis of the loop L1 region of the hexon gene. Nucleotide sequence alignment of this strain, FAdV-7 reference strains B-3A ATCC VR-832 (AF339922) and YR36 (AF508955), and eight additional FAdV-7 field strains confirmed its classification as FAdV-JS-5/10j and showed that these viruses are very similar to each other. Additionally, we described mutations and their influence on the amino acid sequence, nucleotide composition, and relative synonymous codon usage. Immunofluorescence of cell cultures infected with 104.5 TCID 50 per 0.1-ml dose of the FAdV-JSN-5/10j strain demonstrated the presence of a cytopathic effect. Infection of fowl with adenoviruses raises concerns for poultry production, and thus, the efficient detection of adenovirus infection is crucial. This is the first attempt to describe the molecular characteristics of FadV-7 strains isolated in Poland.

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“…The Western blotting analysis was conducted as in our previous work 13. TH expression was quantified with respect to the signals of the corresponding GAPDH band.…”

Section: Methodsmentioning

confidence: 99%

“…They have been found effective in increasing the striatal dopamine content of METH-treated rats 10, 11 and persistently attenuating METH self-administration and relapse in mice 13. However, the neuroprotective effect of CDNF against METH toxicity has been unknown.…”

Section: Introductionmentioning

confidence: 99%

Recombinant AAV8-mediated intrastriatal gene delivery of CDNF protects rats against methamphetamine neurotoxicity

Wang

et al. 2017

Int. J. Med. Sci.

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Methamphetamine (METH) exerts significant neurotoxicity in experimental animals and humans when taken at high doses or abused chronically. Long-term abusers have decreased dopamine levels, and they are more likely to develop Parkinson's disease (PD). To date, few medications are available to treat the METH-induced damage of neurons. Glial cell line-derived neurotrophic factor (GDNF) has been previously shown to reduce the dopamine-depleting effects of neurotoxic doses of METH. However, the effect of cerebral dopamine neurotrophic factor (CDNF), which has been reported to be more specific and efficient than GDNF in protecting dopaminergic neurons against 6-OHDA toxicity, in attenuating METH neurotoxicity has not been determined. Thus, the present study aimed to evaluate the neuroprotective effect of CDNF against METH-induced damage to the dopaminergic system in vitro and in vivo. In vitro, CDNF protein increased the survival rate and reduced the tyrosine hydroxylase (TH) loss of METH-treated PC12 cells. In vivo, METH was administered to rats following human CDNF overexpression mediated by the recombinant adeno-associated virus. Results demonstrated that CDNF overexpression in the brain could attenuate the METH-induced dopamine and TH loss in the striatum but could not lower METH-induced hyperthermia.

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Chimeric hexon HVRs protein reflects partial function of adenovirus

Cited by 18 publications

References 17 publications

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Molecular characterisation of fowl adenovirus type 7 isolated from poultry associated with inclusion body hepatitis in Poland

Recombinant AAV8-mediated intrastriatal gene delivery of CDNF protects rats against methamphetamine neurotoxicity

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