Chimeric mutations in grapevine ENHANCED DISEASE RESISTANCE1 improve resistance to powdery mildew without growth penalty

Abstract

Grapevine (Vitis vinifera L.) incurs severequality degradation and yield loss from powdery mildew, a major fungal disease caused by Erysiphe necator. ENHANCED DISEASE RESISTANCE1 (EDR1), a Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK), negatively regulates defense responses against powdery mildew in Arabidopsis (Arabidopsis thaliana). However, little is known about the role of the putatively orthologous EDR1 gene in grapevine. In this study, we obtained grapevine VviEDR1-edited lines using CRISPR/Cas9. Plantlets containing homozygous and bi-allelic indels in VviEDR1 developed leaf lesions shortly after transplanting into the soil and died at the seedling stage. Transgenic plants expressing wild-type VviEDR1 and mutant Vviedr1 alleles as chimera (designated as VviEDR1-chi) developed normally and displayed enhanced resistance to powdery mildew. Interestingly, VviEDR1-chi plants maintained a spatiotemporally distinctive pattern of VviEDR1 mutagenesis: while almost no mutations were detected from terminal buds, ensuring normal function of the apical meristem, mutations occurred in young leaves and increased as leaves matured, resulting in resistance to powdery mildew. Further analysis showed that the resistance observed in VviEDR1-chi plants was associated with callose deposition, increased production of salicylic acid (SA) and ethylene (ET), H2O2 production and accumulation, and host cell death. Surprisingly, no growth penalty was observed with VviEDR1-chi plants. Hence, this study demonstrated a role of VviEDR1 in the negative regulation of resistance to powdery mildew in grapevine and provided an avenue for engineering powdery mildew resistance in grapevine.

CRISPRingEDR1: Harmonizing grapevine defense and growth dynamics

“…In this issue of Plant Physiology , Yu et al (2024) identified an ortholog of the Arabidopsis EDR1 gene in grapevine ( VviEDR1 ) through multiple sequence alignment and phylogenetic analysis. Expression analysis in susceptible Thompson Seedless grapevine leaves suggests continual downregulation of VviEDR1 after E. necator inoculation, indicating the role of VviEDR1 in grapevine powdery mildew infection.…”

“… Schematic model depicting how VviEDR1-chi lines enhance resistance to powdery mildew without compromising growth (Figure 8 of Yu et al 2024 ). The VviEDR1-chi lines express both the wild-type VviEDR1 and mutant Vviedr1 alleles as chimera ( VviEDR1-chi ), ensuring the dynamic stability of the VviEDR1 mutation.…”