Chimeric Synthetic Peptide as Antigen for Immunodiagnosis of HIV-1 Infection

Hernández, Milenen; Pozo, Lilliam; Gómez, Ivonne; Melchor, Antonio

doi:10.1006/bbrc.2000.2717

Cited by 13 publications

(8 citation statements)

References 16 publications

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“…52%) than that displayed by the RP1ϩRP2ϩRP5 mixture (5.591 versus 3.682, respectively). It has been proposed that peptides in mixtures may decrease their individual antigenicity once adsorbed on solid phases because of the blockage of essential chains (20)(21)(22)(23)47). Another hypothesis to explain these results is that when antigenic peptides present in the mixture adsorb to the well, they compete for the binding sites, leading to sensitivity loss.…”

Section: Selection Of Agsmentioning

confidence: 99%

Comparison of Recombinant Trypanosoma cruzi Peptide Mixtures versus Multiepitope Chimeric Proteins as Sensitizing Antigens for Immunodiagnosis

Camussone

Gonzalez

Belluzo

et al. 2009

Clin Vaccine Immunol

101

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The aim of this work was to determine the best strategy to display antigens (Ags) on immunochemical devices to improve test selectivity and sensitivity. We comparatively evaluated five Trypanosoma cruzi antigenic recombinant peptides, chose the three more sensitive ones, built up chimeras bearing these selected Ags, and systematically compared by enzyme-linked immunosorbent assay the performance of the assortments of those peptides with that of the multiepitope constructions bearing all those peptides lineally fused. The betterperforming Ags that were compared included peptides homologous to the previously described T. cruzi flagellar repetitive Ag (here named RP1), shed acute-phase Ag (RP2), B13 (RP5), and the chimeric recombinant proteins CP1 and CP2, bearing repetitions of RP1-RP2 and RP1-RP2-RP5, respectively. The diagnostic performances of these Ags were assessed for discrimination efficiency by the formula ؉OD/cutoff value (where ؉OD is the mean optical density value of the positive serum samples tested), in comparison with each other either alone, in mixtures, or as peptide-fused chimeras and with total parasite homogenate (TPH). The discrimination efficiency values obtained for CP1 and CP2 were 25% and 52% higher, respectively, than those of their individual-Ag mixtures. CP2 was the only Ag that showed enhanced discrimination efficiency between Chagas' disease-positive and -negative samples, compared with TPH. This study highlights the convenience of performing immunochemical assays using hybrid, single-molecule, chimeric Ags instead of peptide mixtures. CP2 preliminary tests rendered 98.6% sensitivity when evaluated with a 141-Chagas' disease-positive serum sample panel and 99.4% specificity when assessed with a 164-Chagas' disease-negative serum sample panel containing 15 samples from individuals infected with Leishmania spp.

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Section: Selection Of Agsmentioning

confidence: 99%

Comparison of Recombinant Trypanosoma cruzi Peptide Mixtures versus Multiepitope Chimeric Proteins as Sensitizing Antigens for Immunodiagnosis

Camussone

Gonzalez

Belluzo

et al. 2009

Clin Vaccine Immunol

101

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“…As illustrate in Fig. (1), different chemistries to Chimeric peptides [38][39][40][41][42][43][44] Branched polymeric peptides poly[Lys(Ser i-DL -Ala m )] (SAK) [45][46][47] Sequential oligopeptide carriers (SOCn) [48][49][50][51][52] Multiple Antigenic Peptides (MAPs) [53,56,58,[60][61][62][63][64][65][66][67][68] Cyclic peptides [18,27,69,70] …”

Section: Chemical Derivatization Of Synthetic Peptidesmentioning

confidence: 99%

“…However, the use of physical mixtures of synthetic peptides could affect the sensitivity and specificity of the test by the effect of competition for binding to solid phase and for changes in the spatial distribution of antigenic determinants of bound peptides. Therefore, there is a tendency toward using chimeric peptides that contain several epitopes from different proteins within the same chemical molecule to avoid those problems and to improve the sensitivity and specificity of the assays [41]. Published results [36,[42][43][44] demonstrate that chimeric peptides are more antigenic than the monomeric peptides and these can be used to detect antibodies to more than one epitope simultaneously.…”

Section: D Chimeric Peptidesmentioning

confidence: 99%

Synthetic Peptides for the Immunodiagnosis of Human Diseases

Gómara¹,

Haro

2007

CMC

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Synthetic peptides have been shown to be valuable tools for viral laboratory diagnosis and can provide uniform, chemically well-defined antigens for antibody analysis, reducing inter- and intra-assay variation. The main aim in the development of peptide-based diagnostic tests is to recognise specific antibodies induced by the whole viral proteins but using selected short fragments containing the most potent antigenic determinants. The success of this approach depends on the extent to which synthetic peptides are able to mimic the immunodominant epitopes of antigens. In recent years, synthetic peptides that mimic specific epitopes of infectious agents' proteins have been used in diagnostic systems for various human diseases. The present review summarizes some of the drawbacks of the use of relatively short linear peptides as antigenic substrates and the subsequent chemical strategies developed in order to overcome the low peptide reactivity against specific antibodies. Moreover, it outlines the most significant bibliography published in the last five years which provides validated peptide based tests potentially useful for diagnosis of viral, bacterial, parasitic and autoimmune diseases.

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“…There is tendency toward using synthetic chimeric peptides to avoid those problems and improve assay's sensitivity and specificity (17,18).…”

mentioning

confidence: 99%

Antigenicity of Chimeric Synthetic Peptides Based on HTLV-1 Antigens and the Impact of Epitope Orientation

Hernández

Selles²,

Peña³

et al. 2000

Biochemical and Biophysical Research Communications

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Chimeric Synthetic Peptide as Antigen for Immunodiagnosis of HIV-1 Infection

Cited by 13 publications

References 16 publications

Comparison of Recombinant Trypanosoma cruzi Peptide Mixtures versus Multiepitope Chimeric Proteins as Sensitizing Antigens for Immunodiagnosis

Comparison of Recombinant Trypanosoma cruzi Peptide Mixtures versus Multiepitope Chimeric Proteins as Sensitizing Antigens for Immunodiagnosis

Synthetic Peptides for the Immunodiagnosis of Human Diseases

Antigenicity of Chimeric Synthetic Peptides Based on HTLV-1 Antigens and the Impact of Epitope Orientation

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