ChIP-seq predicted estrogen receptor biding sites in human breast cancer cell line MCF7

Li, Qi; Wang, Huichun; Yu, Leyang; Zhou, Jun; Chen, Jingde; Zhang, Xia; Chen, Lin; Gao, Yong; Li, Qun

doi:10.1007/s13277-014-1627-4

Cited by 5 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ChIP-seq assays have confirmed these evidences as presented in studies of AR in prostate cancer cell [127,128] and of ER [129][130][131], GR [132], and PPAR [133] in breast cancer cells. In these investigations authors found new insights into the DNA sequences, in which ones NRs can bind and identify cooperating transcription factors.…”

Section: Chromatin Immunoprecipitation Sequencing (Chip-seq)supporting

confidence: 66%

Investigation of Interactions between DNA and Nuclear Receptors: A Review of the Most Used Methods

Fattori

Indolfo

Campos

et al. 2014

Nuclear Receptor Research

View full text Add to dashboard Cite

Abstract. Nuclear receptors (NRs) comprise a superfamily of proteins modulated by ligands that regulate the expression of target genes. These proteins share a multidomain structure harboring an N-terminal domain, a highly conserved DNA binding domain, and a ligand binding domain, which has ligand dependent activation function. They play key roles in development, metabolism, and physiology being closely related to diseases. Most of the knowledge about this superfamily emerges from investigations on new ligands and are mostly centered in the ligand binding domain. However, more investigation focusing on interactions between DNA and DNA binding domain is necessary to shed light on important roles of NRs' participation in transcriptional mechanisms and in specific genes network. Here, our goal is to discuss some nuances of NRs-DNA interaction, describing details of the most used techniques in this sort of study, such as gel shift (EMSA), DNA footprinting, reporter gene assay, ChIP-Seq, 3C, and fluorescence anisotropy. Additionally, we aim to provide tools, presenting advantages and disadvantages of these common methods, when choosing the most suitable one to study NRs-DNA interactions to answer specific questions.

show abstract

Section: Chromatin Immunoprecipitation Sequencing (Chip-seq)supporting

confidence: 66%

Investigation of Interactions between DNA and Nuclear Receptors: A Review of the Most Used Methods

Fattori

Indolfo

Campos

et al. 2014

Nuclear Receptor Research

View full text Add to dashboard Cite

show abstract

“…Estradiol's stimulation of PAX6 has been noted in MCF7 cell lines. Estradiol is able to stimulate the transcription activity of 187 transcription factors (Li et al, 2014b ), which impresses its involvement in proliferation, migration and cell cycle regulation. Based on these data, it is plausible that Pax-6 may be the facilitator for estradiol-mediated proliferation, which can be seen in the increased proliferative status of GFAP+ NSCs (Figure 6 ).…”

Section: Cytoarchitecture-specific Mechanism Involved In Sexual Diffementioning

confidence: 99%

Estradiol and the Development of the Cerebral Cortex: An Unexpected Role?

Denley¹,

Gatford²,

Sellers³

et al. 2018

Front. Neurosci.

View full text Add to dashboard Cite

The cerebral cortex undergoes rapid folding in an “inside-outside” manner during embryonic development resulting in the establishment of six discrete cortical layers. This unique cytoarchitecture occurs via the coordinated processes of neurogenesis and cell migration. In addition, these processes are fine-tuned by a number of extracellular cues, which exert their effects by regulating intracellular signaling pathways. Interestingly, multiple brain regions have been shown to develop in a sexually dimorphic manner. In many cases, estrogens have been demonstrated to play an integral role in mediating these sexual dimorphisms in both males and females. Indeed, 17β-estradiol, the main biologically active estrogen, plays a critical organizational role during early brain development and has been shown to be pivotal in the sexually dimorphic development and regulation of the neural circuitry underlying sex-typical and socio-aggressive behaviors in males and females. However, whether and how estrogens, and 17β-estradiol in particular, regulate the development of the cerebral cortex is less well understood. In this review, we outline the evidence that estrogens are not only present but are engaged and regulate molecular machinery required for the fine-tuning of processes central to the cortex. We discuss how estrogens are thought to regulate the function of key molecular players and signaling pathways involved in corticogenesis, and where possible, highlight if these processes are sexually dimorphic. Collectively, we hope this review highlights the need to consider how estrogens may influence the development of brain regions directly involved in the sex-typical and socio-aggressive behaviors as well as development of sexually dimorphic regions such as the cerebral cortex.

show abstract