Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

Stavrou, Ioannis J.; Agathokleous, Eliana A.; Kapnissi‐Christodoulou, Constantina P.

doi:10.1002/elps.201600322

Cited by 60 publications

(31 citation statements)

References 163 publications

(212 reference statements)

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“…Separation of structurally similar analytes in CE was useful with additives in the BGE. The additives in the BGE included CDs and their derivatives, crown ether, chiral ligands, surfactants, macrocyclic antibiotics, and ionic liquids (ILs) . ILs are a novel type of additives, which have been widely used in the stereoselective analysis.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of corynoxine and corynoxine B using chiral ionic liquid and hydroxypropyl‐β‐cyclodextrin as additives by field‐amplified sample stacking in capillary electrophoresis

et al. 2018

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A sensitive, fast, and effective method, field-amplified sample stacking (FASS) in capillary electrophoresis, has been established for the separation and determination of corynoxine and corynoxine B. Hydroxypropyl-β-CD (HP-β-CD) and tetrabutylammonium-L-glutamic acid (TBA-L-Glu) were used as additives in the separation system. Electrokinetic injection was chosen to introduce sample from inlet at 10 kV for 50 s after a water plug (0.5 psi, 4 s) was injected to permit FASS. The running buffer (pH 6.1) was composed of 40 mM sodium dihydrogen phosphate solution, 130 mM HP-β-CD, and 10 mM TBA-L-Glu and the separation voltage was 20 kV. Under the optimum conditions, corynoxine and corynoxine B were successfully enriched and separated within 12 min and the sensitivity was improved approximately by 700-900 folds. Calibration curves were in a good linear relationship within the range of 62.5-5.00 × 10 ng/mL for both corynoxine and corynoxine B. The limits of detection (S/N = 3) and quantitation (S/N = 10) were 14.9, 45.2 ng/mL for corynoxine and 11.2, 34.5 ng/mL for corynoxine B, respectively. Finally, this method was successfully applied for the determination of corynoxine and corynoxine B in the stems with hooks of Uncaria rhynchophylla and its formulations.

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Section: Introductionmentioning

confidence: 99%

Separation and determination of corynoxine and corynoxine B using chiral ionic liquid and hydroxypropyl‐β‐cyclodextrin as additives by field‐amplified sample stacking in capillary electrophoresis

et al. 2018

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“…Over the years, various CSs have been utilized in CE including cyclodextrins (CDs) and their derivatives, chiral crown ethers, chiral surfactants, ligand‐exchange complexes, linear polysaccharides like maltodextrins (MDs), and DNA oligonucleotides, to improve the enantioseparation capability of various classes of analytes . However, to improve the resolution of enantiomers, in some cases, dual systems by a combined use of two chiral reagents/modifiers in the BGE are applied for the chiral separation .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the synergistic effect with amino acids for enantioseparation of basic drugs using capillary electrophoresis

et al. 2018

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The synergistic effect of two acidic amino acids, aspartic and glutamic acid, on the electrophoretic enantioseparation of four basic drugs was evaluated in the BGE containing a CD and at different pHs. Chlorpheniramine, hydroxyzine, propranolol and tramadol were used as the basic model drugs. However, no enantioseparations were achieved with a BGE containing sole amino acid, but the combined use of an acidic amino acid and a CD showed improved enantioseparations (synergistic effect) compared with the single CD system. The results demonstrated that at optimized pH, the electrostatic interactions of the anionic amino acids with the positively charged basic drugs could result in a decrease of the analyte migration velocity and it consequently improved the enantioseparation. The effective parameters such as the amino acid and chiral selector type and concentration, buffer pH, applied voltage, and capillary temperature were optimized. Favorable enantiomeric resolution and migration times of the model drugs were achieved with a 100 mM phosphate buffer solution (pH 3.0) containing 5.0 mM HP-α-CD/HP-β-CD and 20 mM aspartic acid with an 18 kV applied voltage at 25°C. H NMR experiments were also carried out in a mixture of an analyte and CD in the absence and presence of aspartic acid. The NMR results were consistent with the results obtained by CE which showed the synergistic effect of amino acid.

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“…CE has proved to be one of the most effective methods for enantiomers' separation due to its high efficiency, robustness, low cost, simplicity, and diversity regarding different modes, such as ACE , MEKC , and NACE . The enantioseparation can be achieved using different types of chiral selectors, such as cyclodextrin and its derivatives , cyclofructans , crown ether , DNA , macrocyclic antibiotics , ionic liquids , chiral metal‐ion complexes , and proteins . However, when using selectors with UV absorbances, such as proteins and DNA, or when the separation is combined with mass spectrometric detection, the bad effects of selectors on detection must be considered.…”

Section: Introductionmentioning

confidence: 99%

Predefine resolution of enantiomers in partial filling capillary electrophoresis and two discontinuous function plugs coupling in‐capillary

et al. 2018

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In common partial filling CE (PF-CE), the capillary contains the selectors plug between the injection and detector end to avoid the selector going into the detector zone. To expand this method, we propose a mode of two discontinuous function plugs coupling in-capillary, named as plug-plug PF-CE (ppPF-CE). Initially, we present the method to predefine the effective length of chiral selector to meet the requirement of enantiomers' resolution, which could avoid some experimental procedures. With α-CD as a chiral selector, a satisfactory resolution of enantiomers d,l-tryptophan and d,l-tyrosine was obtained with a partial filling α-CD plug of optimal length and concentration. Subsequently, a second plug containing hydroxypropyl methylcellulose, organic solvents (acetonitrile and methanol), anionic and cationic surfactants (SDS and CTAB), and different concentrations of sodium phosphate buffer was inserted after the selector plug. Effects of plug-plug filling on enantiomers' migration and resolution are discussed. The ppPF-CE might be a new flexible mode for CE application.

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Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

Cited by 60 publications

References 163 publications

Separation and determination of corynoxine and corynoxine B using chiral ionic liquid and hydroxypropyl‐β‐cyclodextrin as additives by field‐amplified sample stacking in capillary electrophoresis

Separation and determination of corynoxine and corynoxine B using chiral ionic liquid and hydroxypropyl‐β‐cyclodextrin as additives by field‐amplified sample stacking in capillary electrophoresis

Evaluation of the synergistic effect with amino acids for enantioseparation of basic drugs using capillary electrophoresis

Predefine resolution of enantiomers in partial filling capillary electrophoresis and two discontinuous function plugs coupling in‐capillary

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