Chiral separation of N‐methyl‐<scp>dl</scp>‐aspartic acid in rat brain tissue as N‐ethoxycarbonylated (S)‐(+)‐2‐octyl ester derivatives by GC‐MS

Nguyen, Duc-Toan; Kim, Kyoung-Rae; Lee, Gwang; Paik, Man‐Jeong

doi:10.1002/bmc.2703

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…Other methods provide the conversion of each enantiomer into octyl ester derivatives for the direct gas chromatography-mass spectrometry (GC-MS) analysis, as described for the determination of N-methyl- dl -aspartic acid (NMA) [25]. However, most of enantionseparation protocols are based on coupling of liquid chromatography to mass spectrometry as that combining Chirobiotic T chiral stationary phase column to atmospheric pressure chemical ionization (APCI) mass spectrometry for the quantification of 25 amino acid enantiomers [26].…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry

et al. 2017

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Several studies have suggested that free d-Asp has a crucial role in N-methyl d-Asp receptor-mediated neurotransmission playing very important functions in physiological and pathological processes. This paper describes the development of an analytical procedure for the direct and simultaneous determination of free d-Asp, l-Asp and N-methyl d-Asp in specimens of different mouse brain tissues using chiral LC-MS/MS in Multiple Reaction Monitoring scan mode. After comparing three procedures and different buffers and extraction solvents, a simple preparation procedure was selected the analytes of extraction. The method was validated by analyzing l-Asp, d-Asp and N-methyl d-Asp recovery at different spiked concentrations (50, 100 and 200 pg/μl) yielding satisfactory recoveries (75–110%), and good repeatability. Limits of detection (LOD) resulted to be 0.52 pg/μl for d-Asp, 0.46 pg/μl for l-Asp and 0.54 pg/μl for NMDA, respectively. Limits of quantification (LOQ) were 1.57 pg/μl for d-Asp, 1.41 pg/μl for l-Asp and 1.64 pg/μl for NMDA, respectively. Different concentration levels were used for constructing the calibration curves which showed good linearity. The validated method was then successfully applied to the simultaneous detection of d-Asp, l-Asp and NMDA in mouse brain tissues. The concurrent, sensitive, fast, and reproducible measurement of these metabolites in brain tissues will be useful to correlate the amount of free d-Asp with relevant neurological processes, making the LC-MS/MS MRM method well suited, not only for research work but also for clinical analyses.

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Section: Introductionmentioning

confidence: 99%

Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry

et al. 2017

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Analysis of biologically-active, endogenous carboxylic acids based on chromatography-mass spectrometry

Kloos

Lingeman

Mayboroda

et al. 2014

TrAC Trends in Analytical Chemistry

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Liquid chromatography-electrospray ionization-tandem mass spectrometric assay for d-aspartate N-methyltransferase activity in ark shells

Shibata

Hagiya

Okawara

et al. 2020

Bioscience, Biotechnology, and Biochemistry

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A liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the separation and quantification of the enantiomers of N-methylaspartate and N-methylglutamate, after derivatization with Nα-(5-fluoro-2,4-dinitrophenyl)-l-leucinamide was established. The time required for the LC-ESI-MS/MS analysis was within 20 min and the detection limit was approximately 10 fmol per injection, demonstrating that this method can be used for the rapid determination of d-aspartate N-methyltransferase activity in the ark shell clam Scapharca broughtonii. Abbreviations: NMDA: N-methyl-d-aspartate; NMLA: N-methyl-l-aspartate; NMDG: N-methyl-d-glutamate; NMLG: N-methyl-l-glutamate; NMA: N-methylaspartate; NMG: N-methylglutamate; HPLC: high-performance liquid chromatography; SAM: S-adenosyl-l-methionine; OPA: o-phthalaldehyde; LC-ESI-MS/MS: liquid chromatography-electrospray ionization-tandem mass spectrometry; FDLA: Nα-(5-fluoro-2,4-dinitrophenyl)-l-leucinamide; FDAA: Nα-(5-fluoro-2,4-dinitrophenyl)-l-alaninamide; ESI: electrospray ionization; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry; MS/MS: tandem mass spectrometry

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Chiral separation of N‐methyl‐dl‐aspartic acid in rat brain tissue as N‐ethoxycarbonylated (S)‐(+)‐2‐octyl ester derivatives by GC‐MS

Cited by 4 publications

References 22 publications

Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry

Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry

Analysis of biologically-active, endogenous carboxylic acids based on chromatography-mass spectrometry

Liquid chromatography-electrospray ionization-tandem mass spectrometric assay for d-aspartate N-methyltransferase activity in ark shells

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