Chitin contents in different morphological forms ofRhizopus nigricans

Žnidaršič, Polona; Marošek, N.; Pavko, Aleksander

doi:10.1007/bf02816260

Cited by 9 publications

(3 citation statements)

References 14 publications

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“…Gupta et al (2000) suggest that the microbial biomass acts as an ion exchanger by virtue of reactive groups available on the cell surface. It has been reported on several occasions that the cell wall of a species responds to the culture medium and other physical-chemical properties of the environment by greatly changing its composition and chemical-physical properties (Nemcovic and Farkas 2001;Znidarsic et al 1999). The amount and type of C and N sources affect the quantity of structural compounds (chitin-chitosan) and other chemical groups of the cell wall involved in binding dyes (Znidarsic et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Decolourisation of model and industrial dyes by mitosporic fungi in different culture conditions

Anastasi

Prigione

Casieri

et al. 2009

World J Microbiol Biotechnol

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Six mitosporic fungi belonging to five species (Aspergillus flavus var. flavus, Aspergillus ochraceus, Cladosporium cladosporioides, Penicillium glabrum and Penicillium verrucosum) were selected from a screening on 258 fungal strains as the most promising for their ability to remove 2 model dyes in solid conditions. Hence they were tested in liquid conditions for their ability to decolourise 3 model dyes and 9 industrial dyes widely used in the textile industry. The influence of the culture medium, particularly its carbon:nitrogen ratio, on biomass development and decolourisation capacity was considered. All the strains were able to grow in the dyed media and displayed various degrees of decolourisation according to the dye and culture medium. The decolourisation was due to biosorption phenomena. Aspergillus ochraceus performed the highest decolourisation yield being able to remove all dyes over 90%. This strain was also found very effective, both in the living and inactivated form, against simulated effluents that mimicked the recalcitrance of real wastewaters being composed of ten different dyes at high concentration (1,000 ppm), in saline solution.

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Section: Discussionmentioning

confidence: 99%

Decolourisation of model and industrial dyes by mitosporic fungi in different culture conditions

Anastasi

Prigione

Casieri

et al. 2009

World J Microbiol Biotechnol

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“…Moreover, the C : N ratio in the medium can affect the amount of structural compounds and other chemical groups of the cell wall [24]. In this study, Bacillus spp.…”

Section: Resultsmentioning

confidence: 98%

“…On several occasions it has been reported that the cell wall of bacteria responds to the culture medium and other properties of the environment by greatly changing its composition and chemical-physical properties [24, 25]. Moreover, the C : N ratio in the medium can affect the amount of structural compounds and other chemical groups of the cell wall [24].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Chromium Removal by the Indigenous Bacterium Bacillus spp. REP02 Using the Response Surface Methodology

et al. 2011

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An indigenous bacterium, Bacillus REP02, was isolated from locally sourced chromium electroplating industrial effluents. Response surface methodology was employed to optimize the five critical medium parameters responsible for higher % Cr2+ removal by the bacterium Bacillus REP02. A three-level Box-Behnken factorial design was used to optimize K2HPO4, yeast extract, MgSO4, NH4NO3, and dextrose for Cr2+ removal. A coefficient of determination (R 2) value (0.93), model F-value (3.92) and its low P-value (F < 0.0008) along with lower value of coefficient of variation (5.39) indicated the fitness of response surface quadratic model during the present study. At optimum parameters of K2HPO4 (0.6 g L−1), yeast extract (5.5 g L−1), MgSO4 (0.04 g L−1), NH4NO3 (0.20 g L−1), and dextrose (12.50 g L−1), the model predicted 98.86% Cr2+ removal, and experimentally, 99.08% Cr2+ removal was found.

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Dimorphism inBenjaminiella poitrasii: Involvement of intracellular endochitinase andN-acetylglucosaminidase activities in the yeast-mycelium transition

2000

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The chitinase and N-acetylglucosaminidase activities in cell-wall-bound and free fractions in the dimorphic fungus Benjaminiella poitrasii were studied as a function of morphological (unicellular yeast-mycelium) transition. The specific activities of chitinases of cell-wall-free, particularly in the membrane fraction, were significantly different in the yeast and mycelial forms. During the yeast-mycelium transition, the N-acetylglucosaminidase activity isolated in a membrane preparation increased steadily. The activity of the yeast cells (0.83 +/- 0.17 nkat/mg protein) increased 17-fold to 14.2 +/- 1.7 nkat/mg protein in 1-d-old mycelial cells. The endochitinase activity increased 12-fold between 6 and 12 h and thereafter practically remained unchanged up to 24 h. A reverse trend in the chitinolytic activities was observed during the mycelium-yeast transition. Isoelectrofocussing (pH range 3.5-10) of mixed membrane fraction free of particulate fraction of parent and morphological (Y-5, yeast-form) mutant cells separated endochitinase and N-acetylglucosaminidase activity into two pH ranges, viz. 4.3-5.7 and 6.1-7.7, respectively. The predominant N-acetylglucosaminidase activity observed at pH 6.9 and 7.1 for the parent strain membrane fraction was undetected in the mutant preparation. The results suggested that the membrane-bound (either tightly or loosely) chitinolytic enzymes, particularly, N-acetylglucosaminidase, significantly contributed to the morphological changes in B. poitrasii.

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Chitin contents in different morphological forms ofRhizopus nigricans

Cited by 9 publications

References 14 publications

Decolourisation of model and industrial dyes by mitosporic fungi in different culture conditions

Decolourisation of model and industrial dyes by mitosporic fungi in different culture conditions

Optimization of Chromium Removal by the Indigenous Bacterium Bacillus spp. REP02 Using the Response Surface Methodology

Dimorphism inBenjaminiella poitrasii: Involvement of intracellular endochitinase andN-acetylglucosaminidase activities in the yeast-mycelium transition

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