Chitin extraction from shrimp wastes by single step fermentation with
            <i>Lactobacillus acidophilus</i>
            FTDC3871 using response surface methodology

Tan, Joo Shun; Abbasiliasi, Sahar; Lee, Chee Keong; Phapugrangkul, Pongsathon

doi:10.1111/jfpp.14895

Cited by 29 publications

(9 citation statements)

References 39 publications

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“…Lactic acid fermentation can be sufficient for the complete extraction of chitin [ 53 ]. Some species of lactic acid-producing bacteria possess lackluster deproteination capabilities; fermentation with protease-producing cultures can follow to improve the purity of produced chitin [ 54 , 55 , 56 ]. Protease-producing bacteria alone rarely provide adequate demineralization and often require an additional demineralization step [ 55 , 56 , 57 , 58 ].…”

Section: Extraction Methodsmentioning

confidence: 99%

“…The biological method for deacetylation is through the enzyme chitin deacetylase, sourced from fungi, and is similar in execution and limitations to enzymatic methods of chitin extraction [ 11 , 54 ]. This method is rare, as the enzymes are not commercially available and those extracted from biomass suffer from low activity [ 41 , 62 , 63 ].…”

Section: Deacetylationmentioning

confidence: 99%

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Chitin, Chitosan, and Nanochitin: Extraction, Synthesis, and Applications

2022

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Crustacean shells are a sustainable source of chitin. Extracting chitin from crustacean shells is ongoing research, much of which is devoted to devising a sustainable process that yields high-quality chitin with minimal waste. Chemical and biological methods have been used extensively for this purpose; more recently, methods based on ionic liquids and deep eutectic solvents have been explored. Extracted chitin can be converted into chitosan or nanochitin. Once chitin is obtained and modified into the desired form, it can be used in a wide array of applications, including as a filler material, in adsorbents, and as a component in biomaterials, among others. Describing the extraction of chitin, synthesis of chitosan and nanochitin, and applications of these materials is the aim of this review. The first section of this review summarizes and compares common chitin extraction methods, highlighting the benefits and shortcomings of each, followed by descriptions of methods to convert chitin into chitosan and nanochitin. The second section of this review discusses some of the wide range of applications of chitin and its derivatives.

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Section: Extraction Methodsmentioning

confidence: 99%

Section: Deacetylationmentioning

confidence: 99%

Chitin, Chitosan, and Nanochitin: Extraction, Synthesis, and Applications

2022

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“…Several factors including inoculum size was reported as influencing parameters in chitin recovery process from shrimp waste [21]. An increment in deproteinization and demineralization was also observed by increasing the inoculation from 2% to 4% during shrimp Litopenaeus vannamei shells fermentation with L. rhamnoides and B. amyloliquefaciens [22].…”

Section: Effect Of Cell Densitymentioning

confidence: 95%

Investigation of chitin recovery from shrimp waste by yeast fermentation

Bui²,

Trinh³

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study investigated the possibility of using yeast fermentation to transform shrimp waste to chitin for further application. The white leg shrimp head was incubated with three yeast strains Yarrowia lipolytica, Candida tropicalis and Pichia kudriavzevii, in comparison with bacteria Bacillus subtilis and commercial protease Alcalase. The efficacy of fermentation was evaluated through deproteinization and demineralization levels after 2 days. A deproteinization of 80.9% was obtained when incubation with Y. lipolytica which was significantly higher than 76.9% and 65.6% obtained with Alcalase hydrolysis and B. subtilis incubation respectively. Besides, C. tropicalis and P. kudriavzevii expressed a similar low level on deproteinization (31.3-31.7%.) All three yeast showed a good demineralization in range 38.2-49.4% on shrimp head while B. subtilis could demineralize only 16.0%. This primary research shows a potential application of yeast fermentation in chitin recovery from shrimp waste.

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“…However, these deproteinization results are lower than most literature on chitin extraction using biological methods. The lower deproteinization could be associated with some lactic acid bacteria protease enzymes having difficulty accessing the α-chitin structure in the shrimp shells [25]. The use of lactic acid bacteria in single fermentation allows for an inverse relationship between demineralization and deproteinization due to changes in the protease configuration in acidic pH environments, which can affect its activity [23].…”

Section: Chitin Extractionmentioning

confidence: 99%

Screening of protease-producing lactic acid bacteria from Indonesian fermented fish products and its application in chitin extraction from vannamei shrimp (Litopenaeus vannamei) shell

Aisyah,

Gavi,

Putra

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study aimed to screen lactic acid bacteria (LAB) from traditional Indonesian fermented fish products, namely wadi and jambal, with high protease activity for chitin production from Litopenaeus vannamei shrimp shells. Twenty-eight colonies were successfully isolated and identified through biochemical tests such as catalase and Gram staining. Seven out of the twenty-eight colonies were identified as lactic acid bacteria based on their Gram-positive and catalase-negative results. These strains from wadi samples were named GWB 1.3, GWB 1.7, and GWB 2.5, while those from jambal samples were named GMJB 1.3, GMJB 1.6, GMJB 2.6, and GMJB 2.7. The protease activity of these strains was tested using the well-diffusion method on skim milk agar to assess their deproteinized activity. The diameter of the clear zone formed after 24, 48, and 72 hours of incubation was measured. GWB 1.7 and GMJB 2.6 showed the largest clear zone diameter with 21.3 mm and 22.5 mm, respectively, and were selected for the single step-fermentation of shrimp shells. The fermentation process was conducted in 6 days with different inoculum ranging from 0%, 5%, 10%, 15%, and 20% (v/v) as treatments. All treatments, including moisture, ash, and protein, were evaluated for proximate content in the final days. The results indicate that the 20% inoculum showed the highest deproteinization and demineralization for both bacterial strains. GWB 1.7 exhibited a demineralization of 43.7% and deproteinization of 25.2%, while GMJB 2.6 showed a demineralization of 39% and deproteinization of 16.1%. This study demonstrated that lactic acid bacteria from wadi and jambal samples could produce chitin from shrimp shells, a valuable biopolymer with various applications.

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Chitin extraction from shrimp wastes by single step fermentation with Lactobacillus acidophilus FTDC3871 using response surface methodology

Cited by 29 publications

References 39 publications

Chitin, Chitosan, and Nanochitin: Extraction, Synthesis, and Applications

Chitin, Chitosan, and Nanochitin: Extraction, Synthesis, and Applications

Investigation of chitin recovery from shrimp waste by yeast fermentation

Screening of protease-producing lactic acid bacteria from Indonesian fermented fish products and its application in chitin extraction from vannamei shrimp (Litopenaeus vannamei) shell

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