Chitooligosaccharides production from shrimp chaff in chitosanase cell surface display system

Chen, Yanli; Ling, Zhenmin; Mamtimin, Tursunay; Khan, Aman; Peng, Liang; Yang, Jinfeng; Ali, Gohar; Zhou, Tuoyu; Zhang, Qing; Zhang, Jing; Li, Xiangkai

doi:10.1016/j.carbpol.2021.118894

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…immobilized enzyme proteins in a new photosensitive polymer Janus micromotor (JM) based on accelerating diffusion-mediated enzyme–substrate interaction, which absorbs ultraviolet light, protects enzyme activity through magnetic/catalytic movement and accelerates enzyme–substrate degradation. Chen Y et al [ 151 ] expressed and immobilized specific GH8 family CHI-1 enzyme on the surface of Escherichia coli BL21 by cell-surface display technology and successfully produced COS with value-added effects by recombinant DNA technology.…”

Section: Design and Modification Of Enzymementioning

confidence: 99%

Biodegradation and Prospect of Polysaccharide from Crustaceans

et al. 2022

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Marine crustacean waste has not been fully utilized and is a rich source of chitin. Enzymatic degradation has attracted the wide attention of researchers due to its unique biocatalytic ability to protect the environment. Chitosan (CTS) and its derivative chitosan oligosaccharides (COSs) with various biological activities can be obtained by the enzymatic degradation of chitin. Many studies have shown that chitosan and its derivatives, chitosan oligosaccharides (COSs), have beneficial properties, including lipid-lowering, anti-inflammatory and antitumor activities, and have important application value in the medical treatment field, the food industry and agriculture. In this review, we describe the classification, biochemical characteristics and catalytic mechanisms of the major degrading enzymes: chitinases, chitin deacetylases (CDAs) and chitosanases. We also introduced the technology for enzymatic design and modification and proposed the current problems and development trends of enzymatic degradation of chitin polysaccharides. The discussion on the characteristics and catalytic mechanism of chitosan-degrading enzymes will help to develop new types of hydrolases by various biotechnology methods and promote their application in chitosan.

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Section: Design and Modification Of Enzymementioning

confidence: 99%

Biodegradation and Prospect of Polysaccharide from Crustaceans

et al. 2022

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“…Currently, scholars at home and abroad are exploring new wound dressings, and hydrogel is the best candidate for wound dressing because of its moisturizing and drugreleasing properties [5]. COS is an oligomer of chitosan (CS), which has superior biological activity compared to CS, as well as more outstanding physicochemical properties [6]. For example, the solubility of COS is much higher than that of CS, which is easily soluble in water and does not need to be dissolved in acetic acid solution, so it has bright prospects for application in biomedical fields such as wound dressings [7].…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Gel Films of Marine Polysaccharides Cross-Linked with Poly-Metal Ions for Wound Healing

et al. 2022

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The development of an efficient and convenient material to improve skin tissue regeneration is a major challenge in healthcare. Inspired by the theory of moist wound healing, portable chitooligosaccharide (COS)/sodium alginate (SA) dual-net gel films containing multiple metal ions were prepared by a casting and in -situ spray method, which can be used to significantly promote wound healing without the use of therapeutic drugs. A variety of divalent cations was introduced in this experiment to improve the advantages of each metal ion by forming metal ion chelates with COS. Moreover, the physicochemical properties and antioxidant properties of nIon2+-COS/SA gel films were systematically characterized and evaluated by in vitro experiments. The gel films showed good antibacterial activity against Gram-negative and Gram-positive bacteria. In addition, the gel films showed good cytocompatibility in cellular experiments, and the gel films with Zn2+ and Sr2+ addition significantly accelerated wound healing in whole skin defect model experiments. Therefore, this nIon2+-COS/SA gel film is an ideal candidate material for wound dressing.

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“…Currently, the host cells for surface display are mainly concentrated in phage, − bacteria, − yeast, ,− and Bacillus subtilis spores. − However, any traditional cell surface display system has shortcomings. The molecular weight of the polypeptide or protein displayed on the phage cannot be too large; otherwise, it will affect the assembly and infection of the phage.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Novel Cell Surface Display Platform Based on Natural “Chitosan Beads” of Yeast Spores

Wang

et al. 2022

J. Agric. Food Chem.

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Cell surface display technology, which expresses and anchors proteins on the surface of microbial cells, has broad application prospects in many fields, such as protein library screening, biocatalysis, and biosensor development. However, traditional cell surface display systems have disadvantages: the molecular weight of phage display proteins cannot be too large; bacterial display lacks the post-translational modification process for eukaryotic proteins; yeast display is prone to excessive protein glycosylation and misfolding of multisubunit proteins; and the compatibility of Bacillus subtilis spore display needs to be further improved. Therefore, it is extremely valuable to develop an efficient surface display platform with strong universality and stress resistance properties. Although yeast surface display systems have been extensively investigated, the establishment of a surface display platform using yeast spores has rarely been reported. In this study, a novel cell surface display platform based on natural "chitosan beads" of yeast spores was developed. The target protein in fusion with the chitosan affinity protein (CAP) exhibited strong binding capability with "chitosan beads" of yeast spores in vitro and in vivo. Moreover, this protein display system showed highly preferable enzymatic properties and stability. As an example, the displayed LXYL-P1-2-CAP demonstrated high thermostability and reusability (60% of the initial activity after seven cycles of reuse), high storage stability (75% of original activity after 8 weeks), and excellent tolerance to a concentration up to 75% (v/v) organic reagents. To prove the practicability of this surface display system, the semisynthesis of paclitaxel intermediate was demonstrated and its highest conversion rate was 92% using 0.25 mM substrate. This study provides a novel and useful platform for the surface display of proteins, especially for multimeric macromolecular proteins of eukaryotic origin.

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Chitooligosaccharides production from shrimp chaff in chitosanase cell surface display system

Cited by 6 publications

References 45 publications

Biodegradation and Prospect of Polysaccharide from Crustaceans

Biodegradation and Prospect of Polysaccharide from Crustaceans

Multifunctional Gel Films of Marine Polysaccharides Cross-Linked with Poly-Metal Ions for Wound Healing

Establishment of a Novel Cell Surface Display Platform Based on Natural “Chitosan Beads” of Yeast Spores

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