Chitosan: A New Hemostatic

Malette, William G.; Quigley, H. J.; Gaines, Ray D.; Johnson, Norris D.; Rainer, W. Gerald

doi:10.1016/s0003-4975(10)60649-2

Cited by 192 publications

(103 citation statements)

References 3 publications

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“…It is strongly believed that cellular phenotype, type of biomaterial, the nature of the medium, protein absorbance values, and surface charge of the cell membrane are strictly effective in cell culture (33,34,38,39). Serum has been added to chitosan biomaterial in numerous studies in order to promote cellular proliferation (20,31,40).…”

Section: Discussionmentioning

confidence: 99%

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Uslu¹,

Biltekin²,

Denir³

et al. 2015

Balkan Med J

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Background: Chitosan, a linear polysaccharide, has been recently used in biomedical applications. In vitro studies have demonstrated its effect on cellular growth and its stimulatory action on cellular layer formation. Aims: The present study aims to compare the proliferative effects of chitosan in two forms, membranous and solution forms, on Swiss 3T3 mouse embryonic fibroblasts. Study Design: In vitro study. Methods: Three experimental groups were formed: cells were cultured in a normal medium without chitosan (Control Group); cells were cultured either in a medium containing 2.0% chitosan in membranous form (Membrane Group) or chitosan solution at a concentration of 2.0% (Solution Group). Two different methods were used in the experiments: cells cultured on the medium containing chitosan in solution or membranous forms (method 1); and chitosan solution or membranous forms were added into the medium containing previously cultured cells (method 2). Results: Scanning electron microscopic investigations of the experimental groups revealed cells with welldefined cellular projections, intact cellular membranes and tight intercellular junctions. They were especially prominent in the membrane group of method 1 and in the membrane and solution groups of method 2. Mouse monoclonal anti-collagen 1 primary antibody was used to indicate collagen synthesis. Prominent collagen synthesis was detected in the membrane groups on the 10 th day of culture for both methods. Bromodeoxyuridine (BrdU) and MTT assays were performed in order to assess cellular proliferation and viability, respectively. BrdU labelling tests indicated a higher proliferation index in the membrane group of method 1 on the 5 th and 10 th days. For the second method, the membranous form on the 10 th day and solution form on the 5 th day were the most effective groups in terms of cellular proliferation. MTT results reflected a high cellular viability in method 1 on the 5 th day of treatment with the membranous form, whereas cellular viability was highest in the solution form of method 2 on the 5 th day. Conclusion: The membranous form of chitosan induced a significant proliferative effect and increased the ratio of cell-to-cell junctions of Swiss 3T3 mouse embryonic fibroblasts. Conveniently, the solution form also resulted in enhanced cell proliferation and viability compared to the control group. As the solution form is easy to prepare and apply to cells compared to the membrane form, the application of Chitosan directly to media appears to be a convenient alternative for tissue engineering approaches.

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Section: Discussionmentioning

confidence: 99%

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Uslu¹,

Biltekin²,

Denir³

et al. 2015

Balkan Med J

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“…In both animal and clinical studies, it has been shown to provide rapid and effective hemostasis independent of the normal clotting mechanism by stimulating erythrocytes to aggregate (Malette et al, 1983;Vournakis et al, 1997c;Klokkevold et al, 1999), and it appears to invoke a vasoconstrictor as part of the overall hemostatic effect (Cole et al, 1997;Kulling et al, 1998Kulling et al, , 1999Chan et al, 2000;Ikeda et al, 2002). Recently, the pGlcNAc gel has also been utilized in animal studies as an agent for the treatment of gastric varices (Kulling et al, 1998(Kulling et al, , 1999.…”

Section: Discussionmentioning

confidence: 99%

“…A similar product, chitosan, was originally developed for use as a topical hemostatic agent (Malette et al, 1983;Brandenberg et al, 1984). Several studies have shown the effectiveness of chitosan alone or combined with other biomaterials for chemoembolization Nishioka et al, 1992;Wang et al, 1995;Denkbas et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Arterial embolization using poly‐N‐acetyl glucosamine gel in a rat kidney model

Kang

Moreira

et al. 2005

Anat. Rec.

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Aqueous solutions of poly-N-acetyl glucosamine (p-GlcNAc) exhibit a liquid-gel transition at physiological pH and temperature. This feature inspired the authors to conduct a study to evaluate the macro-and histological changes of rat kidneys after embolization using either p-GlcNAc gel injection into the renal artery or ligation of the renal artery. The procedures were performed in 46 rats through open abdominal surgeries. Animals were sacrificed at 3 days and at 1, 3, 5, and 8 weeks postoperatively. The results of both macro-observation and histological study showed that p-GlcNAc gels were effective in causing necrosis and subsequent fibrosis in all embolized kidneys. The data indicate that p-GlcNAc gel may have promise as an effective agent for therapeutic embolization.

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“…It was demonstrated that the dynamic equilibrium linkage between the polymers can be a controllable parameter as well as an interesting property that can be exploited in further experiments. Moreover, the hydrogel has a potential uses as wound dressings and systems for controlled release of drugs, because of the compatibility of PVP with the body fluids, added to the properties of the chitosan as linker and binder [80][81][82], fungicide, and it has a good permeability to gas, immunogenic compatibility, low toxicity and good bioabsorption. Attainment of hydrogels from Schiff base by reaction of poly(N-vinyl-2-coacrolein) and chitosan proved to be an effective methodology for the production of hydrogels.…”

Section: Hydrogels From Poly(n-vinyl-2-co-acrolein) and Chitosan Reacmentioning

confidence: 99%

Hydrogels from Chitosan and a Novel Copolymer Poly(<i>N</i>-Vinyl-2-Pyrrolidone-<i>Co</i>-Acrolein)

Barros¹,

Brant²,

Catalani³

2011

MSA

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Chitosan: A New Hemostatic

Cited by 192 publications

References 3 publications

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Arterial embolization using poly‐N‐acetyl glucosamine gel in a rat kidney model

Hydrogels from Chitosan and a Novel Copolymer Poly(<i>N</i>-Vinyl-2-Pyrrolidone-<i>Co</i>-Acrolein)

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Chitosan: A New Hemostatic

Cited by 192 publications

References 3 publications

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Arterial embolization using poly‐N‐acetyl glucosamine gel in a rat kidney model

Hydrogels from Chitosan and a Novel Copolymer Poly(&lt;i&gt;N&lt;/i&gt;-Vinyl-2-Pyrrolidone-&lt;i&gt;Co&lt;/i&gt;-Acrolein)

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Product

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Hydrogels from Chitosan and a Novel Copolymer Poly(<i>N</i>-Vinyl-2-Pyrrolidone-<i>Co</i>-Acrolein)