Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes

Singh, Rupesh Kumar; Martins, Viviana; Soares, Bruno; Castro, Isaura; Falco, Virgílio

doi:10.3390/ijms21010306

Cited by 36 publications

(45 citation statements)

References 44 publications

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“…Phenolic compounds, which play very important functions during pathogen infection (by e.g., providing mechanical strength through cell wall lignification processes) and in preventing oxidative stress, were also demonstrated to increase following elicitation with chitosan 28 . In fact, total phenolic content, and anthocyanins in particular, have been found to accumulate with chitosan treatment in leaf tissues of lemon balm and basil, and in cell cultures of grape vines 29 – 31 . Chitosan application also enhances the accumulation of several enzymatic antioxidants, including catalase, decreasing malondialdehyde (MDA) levels in leaf tissues thus improving plant antioxidant status 30 , 32 .…”

Section: Introductionmentioning

confidence: 99%

Chitosan increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus) by promoting plant antioxidative metabolism

Silva

Santos

Cruz

et al. 2021

Sci Rep

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The pine wilt disease (PWD), for which no effective treatment is available at the moment, is a constant threat to Pinus spp. plantations worldwide, being responsible for significant economic and environmental losses every year. It has been demonstrated that elicitation with chitosan increases plant tolerance to the pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the PWD, but the biochemical and genetic aspects underlying this response have not been explored. To understand the influence of chitosan in Pinus pinaster tolerance against PWN, a low-molecular-weight (327 kDa) chitosan was applied to mock- and PWN-inoculated plants. Nematode population, malondialdehyde (MDA), catalase, carotenoids, anthocyanins, phenolic compounds, lignin and gene expression related to oxidative stress (thioredoxin 1, TRX) and plant defence (defensin, DEF, and a-farnesene synthase, AFS), were analysed at 1, 7, 14, 21 and 28 days post-inoculation (dpi). At 28 dpi, PWN-infected plants elicited with chitosan showed a sixfold lower nematode population when compared to non-elicited plants. Higher levels of MDA, catalase, carotenoids, anthocyanins, phenolic compounds, and lignin were detected in chitosan-elicited plants following infection. The expression levels of DEF gene were higher in elicited plants, while TRX and AFS expression was lower, possibly due to the disease containment-effect of chitosan. Combined, we conclude that chitosan induces pine defences against PWD via modulation of metabolic and transcriptomic mechanisms related with plant antioxidant system.

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Section: Introductionmentioning

confidence: 99%

Chitosan increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus) by promoting plant antioxidative metabolism

Silva

Santos

Cruz

et al. 2021

Sci Rep

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“…Moreover, in the cv. Tinto Cão, Singh et al [ 57 ] found in chitosan treated leaves and berry skins an up-regulation of several target genes (i.e., PAL , UFGT , ABCC1 , CHS , F3H , ANR , GST , and MATE1 ) that encode key enzymes and transporters involved in secondary metabolic pathways.…”

Section: Grapevine Responses To Biostimulantsmentioning

confidence: 99%

The Role of Biostimulants as Alleviators of Biotic and Abiotic Stresses in Grapevine: A Review

Monteiro

Gonçalves

Cortez

et al. 2022

Plants

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The viticulture and wine industry contribute to the economy and reputation of many countries all over the world. With the predicted climate change, a negative impact on grapevine physiology, growth, production, and quality of berries is expected. On the other hand, the impact of these changes in phytopathogenic fungi development, survival rates, and host susceptibility is unpredictable. Grapevine fungal diseases control has been a great challenge to winegrowers worldwide. The use of chemicals in viticulture is high, which can result in the development of pathogen resistance, increasingly raising concerns regarding residues in wine and effects on human and environmental health. Promoting sustainable patterns of production is one of the overarching objectives and essential requirements for sustainable development. Alternative holistic approaches, such as those making use of biostimulants, are emerging in order to reduce the consequences of biotic and abiotic stresses in the grapevine, namely preventing grape fungal diseases, improving grapevine resistance to water stress, and increasing yield and berry quality.

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“…The results showed that monomeric anthocyanins, catechin, rutin, and quercetin-3-O-galactoside significantly increased in berry skins after treatment with chitosan. In addition, in leaves and berry skins, chitosan treatment upregulated several target genes (i.e., PAL, UFGT, ABCC1, CHS, F3H, ANR, GST, and MATE1) that encode key enzymes and transporters involved in secondary metabolic pathways [200]. On Mouhtaro cv.…”

Section: Chitosanmentioning

confidence: 99%

Biostimulants in Viticulture: A Sustainable Approach against Biotic and Abiotic Stresses

Cataldo

Fucile

Mattii

2022

Plants

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Climate change and disproportionate anthropogenic interventions, such as the excess of phytopharmaceutical products and continuous soil tillage, are jeopardizing viticulture by subjecting plants to continuous abiotic stress. One of the main physiological repercussions of abiotic stress is represented by the unbalanced redox homeostasis due to the overproduction of reactive oxygen species (ROS), ultimately leading to a state of oxidative stress (detrimental to grape quality). To these are added the direct and indirect damages caused by pathogens (biotic stresses). In light of this scenario, it is inevitable that sustainable techniques and sensitivity approaches for environmental and human health have to be applied in viticulture. Sustainable viticulture can only be made with the aid of sustainable products. Biostimulant (PB) applications (including resistance inducers or elicitors) in the vineyard have become interesting maneuvers for counteracting vine diseases and improving grape quality. These also represent a partial alternative to soil fertilization by improving nutrient absorption and avoiding its leaching into the groundwater. Their role as elicitors has important repercussions in the stimulation of the phenylpropanoid pathway by triggering the activation of several enzymes, such as polyphenol oxidase, lipoxygenase, phenylalanine ammonia-lyase, and peroxidase (with the accumulation of phenolic compounds). The present review paper summarizes the PBs’ implications in viticulture, gathering historical, functional, and applicative information. This work aims to highlight the innumerable beneficial effects on vines brought by these products. It also serves to spur the scientific community to a greater contribution in investigating the response mechanisms of the plant to positive inductions.

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Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes

Cited by 36 publications

References 44 publications

Chitosan increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus) by promoting plant antioxidative metabolism

Chitosan increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus) by promoting plant antioxidative metabolism

The Role of Biostimulants as Alleviators of Biotic and Abiotic Stresses in Grapevine: A Review

Biostimulants in Viticulture: A Sustainable Approach against Biotic and Abiotic Stresses

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