Chitosan-Based Bioactive Formulations for the Control of Powdery Mildew in Viticulture

Rosa, David Ruano; Sánchez-Hernández, Eva; Baquero-Foz, Rubén; Martín-Ramos, Pablo; Gil, Jesús Martín; Torres-Sánchez, Sergio; Casanova-Gascón, José

doi:10.3390/agronomy12020495

Cited by 14 publications

(21 citation statements)

References 49 publications

(46 reference statements)

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“…Chitosan oligomers with molecular weight <2000 Da were obtained following the procedure proposed by Santos-Moriano et al [19], with the modifications described in [20].…”

Section: Preparation Of Bark Extracts Chitosan Oligomers and Conjugat...mentioning

confidence: 99%

Bark Extract of Uncaria tomentosa L. for the Control of Strawberry Phytopathogens

et al. 2022

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Gray mold (Botrytis cinerea Pers.), crown and fruit rot (Phytophthora cactorum (Lebert and Cohn) J.Schröt), and verticillium wilt (Verticillium dahliae Kleb.) are among the main diseases that affect the strawberry crop. In the study presented herein, the bark extract of Uncaria tomentosa (Willd. ex Schult.) DC, popularly known as “cat’s claw”, has been evaluated for its capability to act as a sustainable control method. The bioactive compounds present in the aqueous ammonia extract were characterized by gas chromatography–mass spectroscopy, and the antimicrobial activity of the extract—alone and in combination with chitosan oligomers (COS)—was assessed in vitro and as a coating for postharvest treatment during storage. Octyl isobutyrate (30.7%), 19α methyl-2-oxoformosanan-16-carboxylate (9.3%), tetrahydro-2-methyl-thiophene (4.7%), and α-methyl manofuranoside (4.4%) were identified as the main phytoconstituents. The results of in vitro growth inhibition tests showed that, upon conjugation of the bark extract with COS, complete inhibition was reached at concentrations in the 39–93.75 μg∙mL−1 range, depending on the pathogen. Concerning the effect of the treatment as a coating to prolong the storage life and control decay during post-harvest storage, high protection was observed at a concentration of 1000 μg∙mL−1. Because of this effectiveness, higher than that attained with conventional synthetic fungicides, the bark extracts of cat’s claw may hold promise for strawberry crop protection.

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“…Chitosan oligomers with molecular weight <2000 Da were obtained following the procedure proposed by Santos-Moriano et al [19], with the modifications described in [20].…”

Section: Preparation Of Bark Extracts Chitosan Oligomers and Conjugat...mentioning

confidence: 99%

Bark Extract of Uncaria tomentosa L. for the Control of Strawberry Phytopathogens

et al. 2022

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“…Concerning other grapevine diseases, secondary metabolites from S. rochei DSM 41729 and S. lavendofoliae DSM 40217 have also been demonstrated to have antifungal activity against downy mildew [ 57 ]. Such activity would arise from the presence of bioactive substances with antifungal capacity, as discussed in the previous section.…”

Section: Discussionmentioning

confidence: 99%

A Coating Based on Bioactive Compounds from Streptomyces spp. and Chitosan Oligomers to Control Botrytis cinerea Preserves the Quality and Improves the Shelf Life of Table Grapes

Buzón-Durán

Sánchez-Hernández

Sánchez-Báscones

et al. 2023

Plants

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Botrytis cinerea is the most harmful postharvest disease of table grapes. Among the strategies that can be envisaged for its control, the use of coatings based on natural products is particularly promising. The study presented herein focuses on the assessment of the antagonistic capacity of two Streptomyces species and their culture filtrates against B. cinerea. Firstly, the secondary metabolites were characterized by gas chromatography–mass spectrometry, with N1-(4-hydroxybutyl)-N3-methylguanidine acetate and 2R,3S-9-[1,3,4-trihydroxy-2-butoxymethyl]guanine acetate as the main compounds produced by S. lavendofoliae DSM 40217; and cyclo(leucyloprolyl) and cyclo(phenylalanylprolyl) as the most abundant chemical species for S. rochei DSM 41729. Subsequently, the capacity of S. lavendofoliae DSM 40217 and S. rochei DSM 41729 to inhibit the growth of the pathogen was tested in dual culture plate assays, finding 85–90% inhibition. In agar dilution tests, their culture filtrates resulted in effective concentration values (EC90) in the 246–3013 μg·mL−1 range. Upon the formation of conjugate complexes with chitosan oligomers (COS) to improve solubility and bioavailability, a synergistic behavior was observed, resulting in lower EC90 values, ranging from 201 to 953 μg·mL−1. Ex situ tests carried out on ‘Timpson’ and ‘Red Globe’ table grapes using the conjugate complexes as coatings were found to maintain the turgor of the grapes and delay the appearance of the pathogen by 10−15 days at concentrations in the 750−1000 µg·mL−1 range. Hence, the conjugate complexes of COS and the selected Streptomyces spp. culture filtrates may be put forward as promising protection treatments for the sustainable control of gray mold.

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“…Potato dextrose agar (PDA) was provided by Becton Dickinson (Bergen County, NJ, USA). Chitosan oligomers (COS) were prepared according to the procedure published in [14] with the modifications detailed in [15] to obtain a solution with a pH of 4-6 with oligomers having a molecular weight less than 2000 Da and a polydispersity index of 1.6.…”

Section: Chemical Reagentsmentioning

confidence: 99%

Antifungal Activity of Methylxanthines against Grapevine Trunk Diseases

et al. 2022

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Methylxanthines, found in the seeds, leaves, and fruits of some plants, are receiving increasing attention as promising treatments for wood-degrading fungi. The aim of the study presented herein was to explore the potential applications of caffeine, four caffeine derivatives (viz. 8-bromo-caffeine, 8-iodo-caffeine, 8-(4-fluorophenoxy)-caffeine, and 8-(2,3,5,6-tetrafluoroalcoxy)-caffeine), and theophylline as antifungals for Botryosphaeriaceae species associated with grapevine trunk diseases (GTDs). In vitro susceptibility tests were conducted to assess the antimycotic activity of the aforementioned compounds and their conjugated complexes with chitosan oligomers (COS). Caffeine, Br-caffeine, and I-caffeine exhibited higher efficacies than imidazole, the chosen antifungal control. Moreover, a strong synergistic behavior between COS and the methylxanthine derivatives was observed. The COS–I-caffeine complex showed the best overall performance against the phytopathogenic fungi with EC90 values of 471, 640, and 935 µg mL−1 for D. seriata, D. viticola, and N. parvum, respectively. In a second step, combinations of the new treatments with imidazole were also explored, resulting in further activity enhancement and EC90 values of 425, 271, and 509 mL−1 against D. seriata, D. viticola, and N. parvum, respectively, for the COS–I-caffeine-imidazole ternary compound. Given the high in vitro efficacy of these formulations for the control of GTDs, they may deserve further investigation with in vivo and field bioassays as an alternative to conventional fungicides.

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Chitosan-Based Bioactive Formulations for the Control of Powdery Mildew in Viticulture

Cited by 14 publications

References 49 publications

Bark Extract of Uncaria tomentosa L. for the Control of Strawberry Phytopathogens

Bark Extract of Uncaria tomentosa L. for the Control of Strawberry Phytopathogens

A Coating Based on Bioactive Compounds from Streptomyces spp. and Chitosan Oligomers to Control Botrytis cinerea Preserves the Quality and Improves the Shelf Life of Table Grapes

Antifungal Activity of Methylxanthines against Grapevine Trunk Diseases

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