Chitosan Beads as Templates for Layer-by-Layer Assembly and their Application in the Sustained Release of Bioactive Agents

Grech, Jessica M.R.; Mano, João F.; Reis, Rui L.

doi:10.1177/0883911508093389

Cited by 25 publications

(14 citation statements)

References 36 publications

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“…The result confirmed that the layer of GA and AL were successfully coated on the AL70HA30_2 and AL50HA50_2 beads by using the alternate soaking technique. This technique has been reported for surface coating of materials elsewhere . In addition, the alternate soaking of silk fibroin scaffolds in 0.2 M CaCl 2 and 0.12 M Na 2 HPO 4 solutions could result in the deposition of CaPO 4 into silk fibroin porous scaffold for bone tissue engineering application…”

Section: Resultsmentioning

confidence: 99%

“…This technique has been reported for surface coating of materials elsewhere. [33][34][35] In addition, the alternate soaking of silk fibroin scaffolds in 0.2 M CaCl 2 and 0.12 M Na 2 HPO 4 solutions could result in the deposition of CaPO 4 into silk fibroin porous scaffold for bone tissue engineering application. 31 FI-IR Spectra Figure 5 shows the FT-IR spectra of HA, AL, GA, the uncoated AL70HA30 and AL50HA50 beads (AL70HA30_0 and AL50HA50_0) and the AL70HA30 and AL50HA50 beads with two coating layers (AL70HA30_2 and AL50HA50_2).…”

Section: Morphology Of Dried Al/ha Beads and Their Layersmentioning

confidence: 99%

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Beads fabricated from alginate, hyaluronic acid, and gelatin using ionic crosslinking and layer‐by‐layer coating techniques for controlled release of gentamicin

Ratanavaraporn

Chuma

Kanokpanont

et al. 2018

J of Applied Polymer Sci

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The alginate/hyaluronic acid (AL/HA) blended beads at different ratios (AL70HA30, AL50HA50, and AL30HA70) were formed by ionic crosslinking technique. The layer‐by‐layer coating of the beads with two opposite charged molecules, positive‐charged type A gelatin (GA), and negative‐charged AL, using alternate soaking technique was introduced to stabilize the beads. The AL70HA30, AL50HA50 beads showed high stability because of high ionic crosslinked AL component. Two‐layer coating resulted in an appropriate layer that maximized the stability of the AL/HA beads. The higher ratio of HA resulted in the higher water absorption ability but degradation rate was accelerated. An antibiotic gentamicin was loaded into the beads with the entrapment efficiency of 69–89%. The beads containing 30% and 50% HA and coated with two layers showed a sustained release of gentamicin while the release behavior was governed by the diffusion and degradation of the beads. The gentamicin released from all beads also potentially inhibited the growth of Staphylococcus aureus (gram‐positive) and Escherichia coli (gram‐negative) bacteria up to at least 48 h. These beads with antibacterial activity can be further explored for the application as bone void filling material for treatment of infection in bone diseases such as osteoarthritis or rheumatoid. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 46893.

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Section: Resultsmentioning

confidence: 99%

Section: Morphology Of Dried Al/ha Beads and Their Layersmentioning

confidence: 99%

Beads fabricated from alginate, hyaluronic acid, and gelatin using ionic crosslinking and layer‐by‐layer coating techniques for controlled release of gentamicin

Ratanavaraporn

Chuma

Kanokpanont

et al. 2018

J of Applied Polymer Sci

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“…However, administration of physically entrapped rhBMP-2 into the damaged site did not achieve tissue regeneration because of the diffusion and denaturation of rhBMP-2 [17,18]. Additionally, high doses of rhBMP-2 can lead to side effects, such as carcinomas and tumor growth.…”

Section: Introductionmentioning

confidence: 90%

Chitosan Membrane with Surface-bonded Growth Factor in Guided Tissue Regeneration Applications

Chang

Tsao

Chang

et al. 2010

Journal of Bioactive and Compatible Polymers

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The potential of surface covalently bonded rhBMP-2 biodegradable chitosan membrane was examined for guided tissue regeneration (GTR) applications. A chitosan surface-bonded rhBMP-2 membrane was produced via amide bond formation between chitosan and rhBMP-2 using EDC/NHS as the catalyst. The chitosan surface-bonded rhBMP-2 membrane retained more than 70% of the initial rhBMP-2 after 4 weeks of incubation, whereas the chitosan surface-adsorbed rhBMP-2 membrane retained only 30%. The surface-bonded rhBMP-2 did not denature, but expressed sustained biological activity, such as osteoblast cell adhesion, proliferation, and differentiation. X-ray images and histology of an in vivo segmental bone defect rabbit model showed that the chitosan surface-bonded rhBMP-2 membrane induced new bone formation. The chitosan surface-bonded rhBMP-2 membrane has the potential as a bioactive material for GTR.

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“…Estos estudios han analizado la interacción de los polímeros durante la formación de las películas, así como el efecto de la distribución e interacciones de los compuestos sobre las propiedades físicas, antimicrobianas, químicas, entre otras [59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74]. Muchas aplicaciones han sido desarrolladas en campos de la investigación como en el área de electrónica [75][76][77][78][79][80][81][82][83][84][85], la inmovilización de enzimas [86][87][88][89], el desarrollo de nuevos materiales con propiedades ópticas específicas [81,[90][91][92], la liberación de compuestos [93][94][95], entre otras.…”

Section: Conformación De Películas En Multicapasunclassified

Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano

Valderrama

Albarracín

2014

Matéria (Rio J.)

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Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano.Physical, chemical and enzymatic modifications and their effects on the properties of chitosan films. RESUMENLas recientes investigaciones en el campo de biopolímeros han intentado brindar soluciones tecnológicas a los retos en el campo de la ciencia y la tecnología de los materiales. En los últimos años, se han llevado a cabo investigaciones con el objetivo de modificar las propiedades de películas empleando tratamientos enzimáticos y físicos, tales como la aplicación de radiaciones, pulsos eléctricos, plasma, tratamientos térmicos, uso de fluidos supercríticos, así como la conformación de películas en multicapas. Estas modificaciones cambian las propiedades antimicrobianas, físicas y químicas de las películas de quitosano y permiten desarrollar una extensa cantidad de aplicaciones en los campos de biomedicina, farmacología, biotecnología y en la industria de cosméticos y de alimentos. El objetivo del presente artículo de revisión es dar a conocer los nuevos avances en el campo de modificaciones físicas y enzimáticas de películas de quitosano explicando diferentes técnicas empleadas en los últimos años.Palabras clave: quitosano, polímeros, películas modificadas, aditivos alimenticios. ABSTRACTRecent research on biopolymer has focused its efforts to provide technological solutions to current and future challenges in the field of science and technology of materials. The properties of chitosan films have been modified by enzymatic and physical treatments such as the application of radiation, electric pulses, plasma, heat treatments, use of supercritical fluids as well as the formation of multilayer films. These modifications change antimicrobial, physical and chemical properties of chitosan films, and allow developing a wide range of applications in the fields of biomedicine, pharmacology, biotechnology, cosmetic and food industries. The purpose of this review is to present new developments in the field of physical and enzymatic modifications of chitosan films describing the different techniques carried out on the last years.

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Chitosan Beads as Templates for Layer-by-Layer Assembly and their Application in the Sustained Release of Bioactive Agents

Cited by 25 publications

References 36 publications

Beads fabricated from alginate, hyaluronic acid, and gelatin using ionic crosslinking and layer‐by‐layer coating techniques for controlled release of gentamicin

Beads fabricated from alginate, hyaluronic acid, and gelatin using ionic crosslinking and layer‐by‐layer coating techniques for controlled release of gentamicin

Chitosan Membrane with Surface-bonded Growth Factor in Guided Tissue Regeneration Applications

Modificaciones físicas, químicas y enzimáticas y sus efectos sobre las propiedades de las películas de quitosano

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