Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro

Shao, Chunyan; Yu, Ziwei; Luo, Tongwang; Zhou, Bin; Song, Quanjiang; Li, Zhuoyue; Yu, Xiaoqiang; Jiang, Sheng; Zhou, Yingshan; Dong, Wanyu; Wang, Xiaodu; Song, Houhui

doi:10.2147/ijn.s370585

Cited by 25 publications

(10 citation statements)

References 48 publications

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“…Therefore, it is of great practical significance and value to do a good job of preventing and controlling HP‐PRRSV. However, few commercial vaccines are available in the market, and most are inactivated or attenuated vaccines administered by injection 33,34 . It is usually fully protective against PRRSV homologous to vaccine antigens but only partially protective against heterologous PRRSV infection 35 .…”

Section: Discussionmentioning

confidence: 99%

Epitope screening and vaccine molecule design of PRRSV GP3 and GP5 protein based on immunoinformatics

Liu,

Chen

2024

J Cellular Molecular Medi

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Porcine reproductive and respiratory syndrome (PRRS) is a respiratory disease in pigs that causes severe economic losses. Currently, live PRRSV vaccines are commonly used but fail to prevent PRRS outbreaks and reinfection. Inactivated PRRSV vaccines have poor immunogenicity, making PRRSV a significant threat to swine health globally. Therefore, there is an urgent need to develop an effective PRRSV vaccine. This study used immunoinformatics to predict, screen, design and construct a candidate vaccine that fused B‐cell epitopes, CTL‐ and HTL‐dominant protective epitopes of PRRSV strain's GP3 and GP5 proteins. The study identified 12 B‐cell epitopes, 6 CTL epitopes and 5 HTL epitopes of GP3 and GP5 proteins. The candidate vaccine was constructed with 50S ribosomal protein L7/L1 molecular adjuvant, which has antigenicity, solubility, stability, non‐allergenicity and a high affinity for its target receptor, TLR‐3. The C‐ImmSim immunostimulation results showed significant increases in cellular and humoral responses (B cells and T cells) and production of TGF‐β, IL‐2, IL‐10, IFN‐γ and IL‐12. The constructed vaccine was stable and immunogenic, and it can effectively induce strong T‐cell and B‐cell immune responses against PRRSV. Therefore, it is a promising candidate vaccine for controlling and preventing PRRSV outbreaks.

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Section: Discussionmentioning

confidence: 99%

Epitope screening and vaccine molecule design of PRRSV GP3 and GP5 protein based on immunoinformatics

Liu,

Chen

2024

J Cellular Molecular Medi

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“…Alterations in redox homeostasis are critical events that favor viral replication, and many viruses have coevolved to hijack or manipulate this process to promote their own replication and spread . ROS are byproducts of cellular metabolism and overwhelming evidence substantiates that several viral infections induce an increase in intracellular ROS, thus acting on ROS may be a potential antiviral target. , Shao et al previously reported that chitosan-coated Se NPs (CS–Se NPs) could suppress PRRSV-induced apoptosis in MARC-145 cells by ROS-mediated signaling pathways. Tong et al reported that glycyrrhizic acid-based carbon dots (Gly-CDs) could inhibit viral proliferation by suppressing the production of PRRSV-induced ROS .…”

Section: Discussionmentioning

confidence: 99%

“…Selenium nanoparticles (Se NPs) have been proven to show lower toxicity and higher biocompatibility than organic or inorganic Se compounds and therefore may be used as better therapeutic and diagnostic agents for anticancer and antimicrobial treatments. − Se NPs can be synthesized through chemical, physical, and biological methods. Remarkably, increasing evidence has also shown that functional Se NPs can be applied for potential antiviral therapy. , In particular, it has recently been reported that Se NPs can suppress PRRSV-induced apoptosis in MARC-145 cells via the ROS/JNK signaling pathway . These results strongly suggested the potential uses of Se NPs for antiviral treatment.…”

Section: Introductionmentioning

confidence: 95%

“…28,29 In particular, it has recently been reported that Se NPs can suppress PRRSV-induced apoptosis in MARC-145 cells via the ROS/JNK signaling pathway. 30 These results strongly suggested the potential uses of Se NPs for antiviral treatment. Moreover, it is very attractive to synthesize a kind of stronger antiviral nanomaterial by combining the antiviral effects of Zinc oxide nanoparticles and selenium nanoparticles.…”

Section: ■ Introductionmentioning

confidence: 99%

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Zinc Oxide–Selenium Nanoparticles for Inhibiting the Proliferation of Porcine Reproductive and Respiratory Syndrome Virus

Xu,

Li,

Chen

et al. 2024

ACS Appl. Nano Mater.

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Porcine Reproductive and Respiratory Syndrome (PRRS) is an acute, highly contagious swine infectious disease caused by the PRRS virus (PRRSV). Due to the frequent mutation and recombination of PRRSV, the current commercial vaccines cannot provide effective protection, and there is an urgent need to develop antiviral drugs. This study aimed to prepare a kind of zinc oxide−selenium nanoparticle (ZnO−Se NPs) and investigate their inhibitory effect on PRRSV proliferation. The hybridization of ZnO and selenium resulted in the synthesis of ZnO−Se NPs, which were then examined by using dynamic laser light scattering and transmission electron microscopy for characterization. The ZnO−Se NPs showed excellent stability and dispersibility, measuring an average diameter of 90 nm. MTT assay revealed that ZnO−Se NPs had good biocompatibility and low toxicity below 2.5 μg/mL. Various experiments have shown that ZnO−Se NPs have the ability to effectively suppress the proliferation of PRRSV in a dose-dependent fashion. Moreover, this inhibition was equally effective against other subgenotypes of PRRSV and non-RNA viruses, indicating that ZnO−Se NPs had broad-spectrum antimicrobial properties. Mechanism investigations revealed that ZnO−Se NPs inhibited PRRSV primarily by targeting the replication process through upregulation of the expression of NLRX1, a protein that interacts with PRRSV Nsp9, and by inhibiting PRRSV-induced upregulation of ROS, rather than by stimulating the innate immunity. This work emphasized the antiviral impact of ZnO−Se NPs and offered compelling evidence of their potential as a promising therapy against PRRSV and other viral infections.

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“…135,136,143,144 SeNPs in the bare form are highly unstable and tend to aggregate and precipitate and finally turn into grey/black selenium -an inactive form. Therefore, to improve stability and therapeutic effectiveness, SeNPs are stabilized by encapsulation into a suitable matrix/substance like peptides, 141 chitosan, 145 etc. The surface functionalization of SeNPs can influence their physical (size, surface charge) and chemical (toxicity) properties and pharmacokinetics.…”

Section: Non-n-heterocyclic Organoselenium Compoundsmentioning

confidence: 99%

Selenium compounds as promising antiviral agents

Jain,

Priyadarsini

2024

New J. Chem.

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Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro

Cited by 25 publications

References 48 publications

Epitope screening and vaccine molecule design of PRRSV GP3 and GP5 protein based on immunoinformatics

Epitope screening and vaccine molecule design of PRRSV GP3 and GP5 protein based on immunoinformatics

Zinc Oxide–Selenium Nanoparticles for Inhibiting the Proliferation of Porcine Reproductive and Respiratory Syndrome Virus

Selenium compounds as promising antiviral agents

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