Chitosan–gelatin scaffolds for tissue engineering: Physico-chemical properties and biological response of buffalo embryonic stem cells and transfectant of GFP–buffalo embryonic stem cells

Thein-Han, W.W.; Saikhun, Jumnian; Pholpramoo, C.; Misra, R.D.K.; Kitiyanant, Yindee

doi:10.1016/j.actbio.2009.05.012

Cited by 220 publications

(152 citation statements)

References 32 publications

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“…Amongst extracellular matrices, fibronectin was found better than collagen type I and matrigel for growth of primary ES cell colonies suggested that due to the glycoprotein nature, fibronectin posseses membrane-spanning receptor proteins called integrins which play a major role in cell adhesion and maintaining the proliferative ability of buffalo ES cells. To date, no published data is available to discuss the present findings in detail, however, Thein-Han et al (19) reported that 3D porous chitosan scaffolds at different degree of deacetylation (% DD) supported buffalo ESCs and showed chitosan was cytocompatible. Moreover, extracellular matrices (1,20,21) as well as plastic surface (3) and 3D porous natural polymer scaffolds (22) were successfully used for culturing human and buffalo ESCs.…”

Section: Discussionmentioning

confidence: 81%

Developmental Competence of Buffalo (Bubalus bubalis) Pluripotent Embryonic Stem Cells Over Different Homologous Feeder Layers and the Comparative Evaluation with Various Extracellular Matrices

Sharma

Dubey²,

Kumar³

et al. 2013

Int J Stem Cells

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Background and Objectives: Use of somatic cells as a feeder layer to maintain the embryonic stem cells (ESCs) in undifferentiated state limits the stem cell research design, since experimental data may result from a combined ESCs and feeder cell response to various stimuli. Therefore, present study was designed to evaluate the developmental competence of the buffalo ESCs over different homogenous feeders and compare with various extracellular matrices using different concentrations of LIF. Methods and Results: Inner cell masses (ICMs) of in vitro hatched blastocysts were cultured onto homologous feeders viz. fetal fibroblast, granulosa and oviductal cell feeder layers and synthetic matrices viz. fibronectin, collagen type I and matrigel in culture medium. Developmental efficiency was found higher for ESCs cultured on fetal fibroblast and granulosa layers (83.33%) followed by fibronectin (77.78%) at 30 ng LIF. Oviductal feeder was found to be the least efficient feeder showing only 11.11% undifferentiated primary ESC colonies at 30 ng LIF. However, neither feeder layer nor synthetic matrix could support the development of primary colonies at 10 ng LIF. Expression of SSEA-4, TRA-1-60 and Oct-4 were found positive in ESC colonies from all the feeders and synthetic matrices with 20 ng and 30 ng LIF. Conclusions: Fetal fibroblast and granulosa cell while, amongst synthetic matrices, fibronectin were found to be equally efficient to support the growth and maintenance of ESCs pluripotency with 30 ng LIF. This well-defined culture conditions may provide an animal model for culturing human embryonic stem cells in the xeno-free or feeder-free conditions for future clinical applications.

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Section: Discussionmentioning

confidence: 81%

Developmental Competence of Buffalo (Bubalus bubalis) Pluripotent Embryonic Stem Cells Over Different Homologous Feeder Layers and the Comparative Evaluation with Various Extracellular Matrices

Sharma

Dubey²,

Kumar³

et al. 2013

Int J Stem Cells

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“…Chitosan has a good swelling capability in water, and when it is mixed with gelatin, they form the electrostatic interaction between ion amonium (-NH3 + ) from chitosan with carboxylate ion (-COO-) from gelatin [23]. That interaction can affect the ability of water absorption and swelling ratio of the chitosan [24].…”

Section: Maximum Swelling Ratiomentioning

confidence: 99%

“…In this research, the maximal swelling ratio was influenced by cinnamaldehyde concentration [24,25]. Cinnamaldehyde is a good crosslinking agent for chitosan material and gelatine [14,15].…”

Section: Maximum Swelling Ratiomentioning

confidence: 99%

Chitosan-gelatine membrane construct with different cinnamaldehyde concentration as drug delivery system in oral cavity

Ridwan¹,

Purwanti²,

Yulianto³

et al. 2016

AIP Conference Proceedings

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Abstract. The aim of the present study was to develop the chitosan-gelatin membrane for cinnamaldehyde control release since it has a crosslinking effect. Porous membranes with differences cinnamaldehyde concentration as an antiinflammatory agent in the size of ±1.5x1.5 cm, 1 mm thickness and weight around 0.27 g were prepared for measure the swelling ratio and weight loss. Scanning electron microscopy was carried out to describe the surface structure, and the chemical bonding was investigated by Fourier Transformed Infrared (F-TIR). The one way ANOVA and LSD analysis of the swelling ratio and weight loss demonstrated that there were significant differences between all cinnamaldehyde groups and a control group (p<0.05).

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“…This may be due to the drug-matrix weak interaction. films CG 1-1, CG 1-3, CGT 1-1, CGT 1-3 The composite films show weak diffraction peaks in the range [11][12][13][14][15][16][17][18][19][20][21][22][23][24] o characteristics of chitosan and gelatin 30,33 and implying predominant amorphous character of composite films like prestine polymers. In the composite films with and without drugs the diffraction peaks are broadened indicating weak interactions between chitosangelatin and matrix-drug.…”

Section: Tga/dtgmentioning

confidence: 99%

“…Gelatin, a protein derived from collagen and bones is translucent, colorless, brittle and tasteless, promotes general joint health and stiffness in athletes. It has good film forming property and known for its wound healing properties by preventing fluid loss due to exudation 13,[22][23][24][25][26] .…”

mentioning

confidence: 99%

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2014

IJPSR

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Natural polymer composite films from a mixture of chitosan and gelatin of various compositions were solution casted using acetic acid with and without theophylline. The films were characterized by FT-IR, TG, differential scanning calorimetry, SEM, XRD and swellability in phosphate buffer. SEM micrographs indicated uniform dispersion of the drug in the polymer blend and drug crystals were seen at higher magnification. TG, DSC and FT-IR studies implied polymerpolymer and polymer-drug weak interactions in the casted film. In vitro transdermal delivery of drug from theophylline loaded films were evaluated spectrophotometrically in phosphate buffer (pH=5.4) using Franz diffusion cell. The study revealed that at initial stage of release (upto 6h) there was only moderate change on the drug release profiles with increased content of gelatin in the film. But at higher release times (10h) the release rate was enhanced with increased gelatin-chitosan ratio. This was attributed to the improved swellability of the film in the buffer. The drug release followed a non-Fickian mechanism. The experimental observations implied that the gelatin-chitosan composite film may be used as transdermal carrier for systemic delivery of drug via the skin.

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Chitosan–gelatin scaffolds for tissue engineering: Physico-chemical properties and biological response of buffalo embryonic stem cells and transfectant of GFP–buffalo embryonic stem cells

Cited by 220 publications

References 32 publications

Developmental Competence of Buffalo (Bubalus bubalis) Pluripotent Embryonic Stem Cells Over Different Homologous Feeder Layers and the Comparative Evaluation with Various Extracellular Matrices

Developmental Competence of Buffalo (Bubalus bubalis) Pluripotent Embryonic Stem Cells Over Different Homologous Feeder Layers and the Comparative Evaluation with Various Extracellular Matrices

Chitosan-gelatine membrane construct with different cinnamaldehyde concentration as drug delivery system in oral cavity

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