Chitosan gold nanoparticles for detection of amplified nucleic acids isolated from sputum

Tammam, Salma N.; Khalil, Mahmoud A F; Gawad, Eman Abdul; Althani, Asma; Zaghloul, Hosam; Azzazy, Hassan M.E.

doi:10.1016/j.carbpol.2017.01.051

Cited by 29 publications

(12 citation statements)

References 55 publications

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“…In a previous study we have investigated a fast synthesis method to realize polymer-modified AuNPs using dycarboxylic PEG, collagen or bisphosphonate (BPO) as stabilizers in order to demonstrate high stability and efficacy under realistic biomedical conditions 5,6,7,8 . Other authors have synthetized hybrid metal nanoparticles based on chitosan and chitosan-derivatives as sugar stabilizers for various applications in the field of the nanomedicine 9,10,11,12,13 .…”

Section: Introductionmentioning

confidence: 99%

Lactose-Modified Chitosan Gold(III)-PEGylated Complex-Bioconjugates: From Synthesis to Interaction with Targeted Galectin-1 Protein

et al. 2018

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Galectins (Gal) are a family of glycan-binding proteins characterized by their affinity for β-galactosides. Galectin-1 (Gal-1), a dimeric lectin with two galactoside-binding sites, regulates cancer progression and immune responses. Coordination chemistry has been engaged to develop versatile multivalent neoglycoconjugates for binding Gal-1. In this study we report a fast and original method to synthesize hybrid gold nanoparticles in which a hydrochloride lactose-modified chitosan, named CTL, is mixed with dicarboxylic acid-terminated polyethylene glycol (PEG), leading to shell-like hybrid polymer-sugar-metal nanoparticles (CTL-PEG-AuNPs). The aim of this paper is to preliminarily study the interaction of the CTL-PEG-AuNPs with a target protein, namely, Gal-1, under specific conditions. The molecular interaction has been measured by Transmission Electron Microscopy (TEM), UV-vis, and Raman Spectroscopy on a large range of Gal-1 concentrations (from 0 to 10 M). We observed that the interaction was strongly dependent on the Gal-1 concentration at the surface of the gold nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Lactose-Modified Chitosan Gold(III)-PEGylated Complex-Bioconjugates: From Synthesis to Interaction with Targeted Galectin-1 Protein

et al. 2018

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“…CS@AuNPs were prepared using a one‐step method according to a previous report with minor modifications . Specifically, 50 mg chitosan was dissolved in 50 mL 1% (v/v) acetic acid, and 0.5 ml (0.01 g mL −1 ) HAuCl 4 .3H 2 O solution was added to the mixture, which was thoroughly mixed.…”

Section: Methodsmentioning

confidence: 99%

“…AuNPs possess many beneficial properties including convenience in synthesis and labelling, a large extinction coefficient, and strong surface plasmon resonance . Therefore, the application of functionalized AuNPs in biological analysis and biomedicine has attracted wide interest.…”

Section: Introductionmentioning

confidence: 99%

A dual‐readout nanosensor based on biomass‐based C‐dots and chitosan@AuNPs with hyaluronic acid for determination of hyaluronidase

et al. 2019

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A dual-signal strategy is proposed based on fluorescent biomass-based carbon dots (BC-dots) and chitosan stabilized AuNPs (CS@AuNPs) to determine hyaluronidase (HAase). BC-dots can induce aggregation of CS@AuNPs nanoparticles with a colour change from red to blue. Positively charged CS@AuNPs interacted with the negatively charged hyaluronic acid (HA) through electrostatic adsorption, and CS@AuNPs maintained stability due to the semirigid coil conformation of HA. However, in the presence of HAase, due to enzymatic hydrolysis of HA by HAase, the CS@AuNPs agglomerated. Based on the change of fluorescence and colour, quantitative analysis of HAase was achieved. Linear ranges for the fluorometric and colorimetric determinations were 2.0-70 U mL −1 and 8-60 U mL −1 , respectively, with a detection limit of 0.27 U mL −1 . This dual-signal sensing system possesses high potential for determination of HAase in biological matrices.

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“…For example, AuNPs coated with chitosan have been used to detect Mycobacterium tuberculosis. Differentiation between positive and negative Mycobacterium tuberculosis samples was possible by a color change in the system [221].…”

Section: Treatment Of Other Diseasesmentioning

confidence: 99%

Nanoengineering of Gold Nanoparticles: Green Synthesis, Characterization, and Applications

et al. 2019

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The fundamental aspects of the manufacturing of gold nanoparticles (AuNPs) are discussed in this review. In particular, attention is devoted to the development of a simple and versatile method for the preparation of these nanoparticles. Eco-friendly synthetic routes, such as wet chemistry and biosynthesis with the aid of polymers, are of particular interest. Polymers can act as reducing and/or capping agents, or as soft templates leading to hybrid nanomaterials. This methodology allows control of the synthesis and stability of nanomaterials with novel properties. Thus, this review focus on a fundamental study of AuNPs properties and different techniques to characterize them, e.g., Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), UV-Visible spectroscopy, Dynamic Light Scattering (DLS), X-Ray Diffraction (XRD), X-Ray Photoelectron Spectroscopy, Small-angle X-Ray Scattering (SAXS), and rheology. Recently, AuNPs obtained by “green” synthesis have been applied in catalysis, in medicine, and as antibacterials, sensors, among others.

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Chitosan gold nanoparticles for detection of amplified nucleic acids isolated from sputum

Cited by 29 publications

References 55 publications

Lactose-Modified Chitosan Gold(III)-PEGylated Complex-Bioconjugates: From Synthesis to Interaction with Targeted Galectin-1 Protein

Lactose-Modified Chitosan Gold(III)-PEGylated Complex-Bioconjugates: From Synthesis to Interaction with Targeted Galectin-1 Protein

A dual‐readout nanosensor based on biomass‐based C‐dots and chitosan@AuNPs with hyaluronic acid for determination of hyaluronidase

Nanoengineering of Gold Nanoparticles: Green Synthesis, Characterization, and Applications

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