Chitosan/Graphene Oxide Nanocomposite Coatings on Mg alloy: Corrosion and Biocompatibility Properties

Abstract

In the present study, optimal conditions for the fabrication of chitosan (CS)/graphene oxide (GO) nanocomposite coatings were evaluated by the pulse electrodeposition process (PED) on Mg–2wt%Zn scaffolds. The size distribution of CS, GO, and nanocomposite coatings were evaluated using dynamic light scattering (DLS). The coatings microstructure and morphologies were investigated by the Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV- visible), Raman spectroscopy, thermogravimetric analysis (TGA), derivative thermal gravimetric (DTG), differential thermal analysis (DTA), and scanning electron microscopy (SEM). Taguchi statistical method was used to optimize the effect of PED parameters, including peak current density (CD) and duty cycle (DC) as well as GO nanosheets content (1,2, and 3wt%). Results showed that optimal coatings were produced under the conditions of 2 wt% GO, CD = 20 mA/cm 2, DC = 0.5 and pH = 5. The process's time, temperature, and frequency were 20 min, 37oC, and 1000 Hz. The biocompatibility of coatings was assessed by in vitro test. The results of cell viability and adhesion of MG63 cells on optimal coating are promising for application in bone tissue engineering.

Nanoscale hydroxyapatite/chitosan polymer coatings: Synthesis and characterization

“…The vertical placement of a syringe was employed to apply a 4 μL droplet of deionized water onto a 1 cm 2 sheet of every scaffold surface. The biocompatibility of the scaffold and coatings was evaluated in accordance with our previous researches through quantitative toxicity tests on the MG-63 osteoblastic line cultures [35][36][37] that were gathered from the Iran Pasteur Institute's national cell collection.…”