Chitosan hydrogel in combination with marine peptides from tilapia for burns healing

Ouyang, Qianqian; Zhang, Hu; Lin, Zhenpeng; Quan, Wei-Yan; Deng, Yifeng; Li, Sidong; Li, Puwang; Chen, Yu

doi:10.1016/j.ijbiomac.2018.01.217

Cited by 100 publications

(73 citation statements)

References 46 publications

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“…Anyway, also in L929 cells, the qualitative wound-healing score assigned in blind (Table 2) revealed an increased cell migration/density in M4 and M5-treated cells both at 24 h and 30 h (second and third column, respectively) compared to the controls (CT) at the same time points. The results from both cell lines are quite comparable to those obtained by Hu et al [40] and Ouyang et al [63] on the same cell lines, HaCaT and L929, respectively, in both papers treated with marine collagen peptides from Nile Tilapia, with [63] and without [40] the concomitant use of chitosan. In both cases, a similar increase of wound closure was obtained at the same time and at the same concentration of peptides used by us in the two cell lines tested.…”

Section: Resultssupporting

confidence: 86%

“…The use of marine collagen in composite biomaterials as wound dressing to enhance healing has sporadically been reported [60,61], as well as the use of marine collagen peptides alone or in combined biomaterials demonstrating wound-healing properties in vivo [40,45,62,63]. Thus, the four MCH fractions were also tested for their wound-healing properties both in HaCaT keratinocytes and in L929 fibroblasts.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the four MCH fractions were also tested for their wound-healing properties both in HaCaT keratinocytes and in L929 fibroblasts. Cell migration/proliferation was performed by the “scratch” assay, which is an in vitro test that is widely used for these purposes [40,63,64], and is described in detail in the Materials and Methods section. The assay was performed in the presence or absence of the different MCH fractions at a concentration of 50 µg/mL.…”

Section: Resultsmentioning

confidence: 99%

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Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

et al. 2018

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Recently, the bioactive properties of marine collagen and marine collagen hydrolysates have been demonstrated. Although there is some literature assessing the general chemical features and biocompatibility of collagen extracts from marine sponges, no data are available on the biological effects of sponge collagen hydrolysates for biomedical and/or cosmetic purposes. Here, we studied the in vitro toxicity, antioxidant, wound-healing, and photoprotective properties of four HPLC-purified fractions of trypsin-digested collagen extracts—marine collagen hydrolysates (MCHs)—from the marine sponge C. reniformis. The results showed that the four MCHs have no degree of toxicity on the cell lines analyzed; conversely, they were able to stimulate cell growth. They showed a significant antioxidant activity both in cell-free assays as well as in H2O2 or quartz-stimulated macrophages, going from 23% to 60% of reactive oxygen species (ROS) scavenging activity for the four MCHs. Finally, an in vitro wound-healing test was performed with fibroblasts and keratinocytes, and the survival of both cells was evaluated after UV radiation. In both experiments, MCHs showed significant results, increasing the proliferation speed and protecting from UV-induced cell death. Overall, these data open the way to the use of C. reniformis MCHs in drug and cosmetic formulations for damaged or photoaged skin repair.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

et al. 2018

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“…Bioactive peptides are a major resource and there are nearly 50 types of bioactivity, with a total number of 3566 bioactive peptides, according to the ScienceDirect database and BIOPEP. In recent years, there have been an increasing number of studies of ACE inhibitory activity, which have found that most tilapia peptides display antioxidant, antihypertensive and burn healing properties, with the ACE inhibitory peptide being the most notable . The peptide LSGYGP from tilapia skin gelatin hydrolysates has been purified, and its molecular weight is 592.26 Da .…”

Section: Introductionmentioning

confidence: 99%

Comparison of an angiotensin‐I‐converting enzyme inhibitory peptide from tilapia (Oreochromis niloticus) with captopril: inhibition kinetics, in vivo effect, simulated gastrointestinal digestion and a molecular docking study

et al. 2019

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BACKGROUND: In order to utilize tilapia skin gelatin hydrolysate protein, which is normally discarded as industrial waste in the process of fish manufacture, we study the in vivo and in vitro angiotensin-I-converting enzyme (ACE) inhibitory activity of the peptide Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP). The aim was to provide a pharmacological basis of the development of minimal side effects of ACE inhibitors by comparative analysis with captopril in molecular docking. RESULTS:This peptide from protein-rich wastes showed excellent ACE inhibitory activity (IC 50 = 2.577 mol L −1 ) and exhibited a mixed noncompetitive inhibitory pattern with Lineweaver-Burk plots. Furthermore, LSGYGP and captopril groups both showed significant decreases in blood pressure after 6 h and maintained good digestive stability over 4 h. Molecular bond interactions differentiate competitive captopril upon hydrogen bond interactions and Zn(II) interaction. The C-terminal Pro generates three interactions (hydrogen bonds, hydrophilic interactions and Van der Waals interactions) in the peptide and effectively interactswith the S1 and S2 pockets of ACE. CONCLUSION: LSGYGP, with an IC 50 value of 2.577 mol L −1 , has an antihypertensive effect in spontaneously hypertensive rats. Through comparison with captopril, this study revealed that LSGYGP may be a potential food-derived ACE inhibitory peptide and could act as a functional food ingredient to prevent hypertension.

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“…Chen et al reported that the application of type I collagen from tilapia skin on dorsal wounds of SD rats accelerated wound healing by reducing inflammatory infiltration, promoting fibroblasts proliferation, collagen synthesis, and re-epithelialization via upregulation of EGF, FGF, and CD31 in the skin tissue [ 10 ]. In rabbits with burn wounds applied with tilapia skin, collagen-derived peptides, combined with chitosan, demonstrated healing by promoting re-epithelialization, collagen fiber deposition, and the upregulation of FGF2 and VEGF in the skin tissue [ 40 ]. In our current study, we observed that the topical application of SCC and TSC significantly increased the levels of the above four growth factors in the skin wound, with a clearly different expressing pattern.…”

Section: Discussionmentioning

confidence: 99%

Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin

Lai

Kuo

et al. 2020

Marine Drugs

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Type II collagen is an important component of cartilage; however, little is known about its effect on skin wound healing. In this study, type II collagen was extracted from the cartilage of Acipenser baerii and its effect on in vitro and in vivo wound healing was compared to type I collagen derived from tilapia skin. Sturgeon cartilage collagen (SCC) was composed of α1 chains and with a thermal denaturation (Td) at 22.5 and melting temperature (Tm) at 72.5 °C. Coating SCC potentiated proliferation, migration, and invasion of human dermal fibroblast adult (HDFa) cells. Furthermore, SCC upregulated the gene expression of extracellular matrix (ECM) components (col Iα1, col IIIα1, elastin, and Has2) and epithelial-mesenchymal transition (EMT) molecules (N-cadherin, Snail, and MMP-1) in HDFa. Pretreatment with Akt and mitogen-activated protein kinase (MAPK) inhibitors significantly attenuated the HDFa invasion caused by SCC. In mice, the application of SCC on dorsal wounds effectively facilitated wound healing as evidenced by 40–59% wound contraction, whereas the untreated wounds were 18%. We observed that SCC reduced inflammation, promoted granulation, tissue formation, and ECM deposition, as well as re-epithelialization in skin wounds. In addition, SCC markedly upregulated the production of growth factors in the dermis, and dermal and subcutaneous white adipose tissue; in contrast, the administration of tilapia skin collagen (TSC) characterized by typical type I collagen was mainly expressed in the epidermis. Collectively, these findings indicate SCC accelerated wound healing by targeting fibroblast in vitro and in vivo.

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Chitosan hydrogel in combination with marine peptides from tilapia for burns healing

Cited by 100 publications

References 46 publications

Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

Comparison of an angiotensin‐I‐converting enzyme inhibitory peptide from tilapia (Oreochromis niloticus) with captopril: inhibition kinetics, in vivo effect, simulated gastrointestinal digestion and a molecular docking study

Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin

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