Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli

Abstract

This study aims to investigate the effects of dietary chitosan (COS) on gastrointestinal pathogen resistance in mice model. For two weeks, a control group of ICR mice received a basal diet whilst the intervention group received the basal diet supplemented with 300 mg/kg COS. After two weeks, the mice fed the supplemented diet had a lower body weight. Then enterotoxigenic Escherichia coli (E. coli) was administered to the mice through oral gavage, with each mouse receiving 108 CFU. At day 7 after infection, the bacterial load in the jejunum and faeces was significantly lower in the COS group than that in the control group. Moreover, the mRNA and protein levels of IL-1β, IL-6, IL-17, IL-18, and TNF-α were significantly lower in the group of mice receiving the COS diet; also the jejunal production of toll-like receptor-4 (TLR-4) was suppressed in the COS group. These results indicate the intervention influenced inflammation and controlled E. coli infection.

Effects of microencapsulated Lactobacillus plantarum and fructooligosaccharide on growth performance, blood immune parameters, and intestinal morphology in weaned piglets

“…Many studies have shown that synbiotics are good substitutes for antibiotics in feed (Estrada, Drew, & Van Kessel, 2001;Guerra-Ordaz et al, 2013). The reasons are that probiotics and prebiotics can synergistically promote growth of animals as well as improve their resistance to infections by equilibrating intestinal microbiota and stimulating the immune system (Fan, Chang, Yin, Wang, & Dang, 2015;Guerra-Ordaz et al, 2013;Liu et al, 2016). A previous study suggested that synbiotics containing L. plantarum and lactulose increased ADG in weaned pigs (Guerra-Ordaz et al, 2014).…”

Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice

“…Western Blotting Analysis. Total proteins extracted from brain samples were used for western blotting determination as previously described [16]. Briefly, 20 μg protein per lane was separated by SDS-PAGE and then blotted onto PVDF membranes.…”

Inhibition of NF-κB Signaling Pathway by Resveratrol Improves Spinal Cord Injury

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