Chitosan nanomagnets for effective extraction and sensitive mass spectrometric detection of pathogenic bacterial endotoxin from human urine

Gopal, Judy; Abdelhamid, Hani Nasser; Hua, Pei-Yang; Wu, Hui‐Fen

doi:10.1039/c3tb20079e

Cited by 94 publications

(48 citation statements)

References 77 publications

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“…Selection of the optimal functionalization mode of MNPs is the most important parameter in MSPE and different types were assayed, including oleic acid , polystyrene , polyaniline , polypyrrole , chitosan , (3‐aminopropyl)triethoxysilane , and polydopamine .…”

Section: Resultsmentioning

confidence: 99%

Magnetic solid‐phase extraction or dispersive liquid–liquid microextraction for pyrethroid determination in environmental samples

Pastor-Belda

Navarro-Jiménez

Garrido

et al. 2018

J of Separation Science

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The determination of 15 pyrethroids in soil and water samples was carried out by gas chromatography with mass spectrometry. Compounds were extracted from the soil samples (4 g) using solid-liquid extraction and then salting-out assisted liquid-liquid extraction. The acetonitrile phase obtained (0.8 mL) was used as a dispersant solvent, to which 75 μL of chloroform was added as an extractant solvent, submitting the mixture to dispersive liquid-liquid microextraction. For the analysis of water samples (40 mL), magnetic solid-phase extraction was performed using nanocomposites of magnetic nanoparticles and multiwalled carbon nanotubes as sorbent material (10 mg). The mixture was shaken for 45 min at room temperature before separation with a magnet and desorption with 3 mL of acetone using ultrasounds for 5 min. The solvent was evaporated and reconstituted with 100 μL acetonitrile before injection. Matrix-matched calibration is recommended for quantification of soil samples, while water samples can be quantified by standards calibration. The limits of detection were in the range of 0.03-0.5 ng/g (soil) and 0.09-0.24 ng/mL (water), depending on the analyte. The analyzed environmental samples did not contain the studied pyrethroids, at least above the corresponding limits of detection.

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Section: Resultsmentioning

confidence: 99%

Magnetic solid‐phase extraction or dispersive liquid–liquid microextraction for pyrethroid determination in environmental samples

Pastor-Belda

Navarro-Jiménez

Garrido

et al. 2018

J of Separation Science

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“…The Fe 3 O 4 @chitosan nanomagnet approach gave 67× higher sensitivity at 450 mg mL −1 (2.25 mg, 5 mL) compared to the direct MALDI‐MS analysis. CuFeO 2 @chitosan nanomagnets appeared to be about 4× more effective than Fe 3 O 4 @chitosan nanomagnets and 250× more sensitive than the direct MALDI approach (Gopal et al, ).…”

Section: Studies On Specific Carbohydrate Typesmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Harvey

2018

Mass Spectrometry Reviews

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This review is the eighth update of the original article published in 1999 on the application of Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2014. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, and arrays. The second part of the review is devoted to applications to various structural types such as oligo- and poly- saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2018 Wiley Periodicals, Inc. Mass Spec Rev 37:353-491, 2018.

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“…Evidently, these results confirmed that the NP-based MALDI-MS can be used in complementary enrichment platforms to increase and to expand the library of protein peaks in bacteria species, which is useful in profiling bacterial species from clinical samples. Furthermore, Fe 3 O 4 -chitosan and CuFeO 2 -chitosan NMs were used as affinity probes for preconcentration and enrichment of trace-level endotoxins (lipopolysaccharides) from human-urine samples [121]. It was observed that the Fe 3 O 4 -chitosan NMs exhibited 67 times higher sensitivity (2.25 μg, 5 μL) compared to direct MALDI-MS analysis.…”

Section: Nanomaterials-based Maldi-ms For Bacterial Identification Anmentioning

confidence: 97%

Nanomaterial-based miniaturized extraction and preconcentration techniques coupled to matrix-assisted laser desorption/ionization mass spectrometry for assaying biomolecules

Chitosan nanomagnets for effective extraction and sensitive mass spectrometric detection of pathogenic bacterial endotoxin from human urine

Cited by 94 publications

References 77 publications

Magnetic solid‐phase extraction or dispersive liquid–liquid microextraction for pyrethroid determination in environmental samples

Magnetic solid‐phase extraction or dispersive liquid–liquid microextraction for pyrethroid determination in environmental samples

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Nanomaterial-based miniaturized extraction and preconcentration techniques coupled to matrix-assisted laser desorption/ionization mass spectrometry for assaying biomolecules

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