Chitosan Nanoparticles for Topical Co-administration of the Antioxidants Glutathione and Idebenone: Characterization and In vitro Release

Montenegro, Lucía; Trapani, Adriana; Fini, Paola; Mandracchia, Delia; Latrofa, Andrea; Cioffi, Nicola; Chiarantini, Laura; Picceri, Giusi; Brundu, Serena; Puglisi, Giovanni

doi:10.9734/bjpr/2014/8641

Cited by 11 publications

(12 citation statements)

References 24 publications

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“…DPPH radical has a deep violet colour in solution, and gradually it became pale yellow in the presence of non-encapsulated GSH. As reported in Table 2, GSH aqueous solution taken as control provided 100% activity against DPPH radical as already observed by us [18]. In addition, both SLN containing GSH demonstrated 100% antioxidant activity.…”

Section: In Vitro Antioxidant Activity Of Gsh-slnsupporting

confidence: 82%

“…The in vitro antioxidant activity of selected GSH-SLN, unloaded SLN and pure GSH was evaluated according to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical test [18][19][20]. The DPPH· was dissolved in ethanol at the concentration of 0.001% (w/v) to obtain a stock solution and, then, diluted up to reach the concentration equal to 8 × 10 −4 % (w/v).…”

Section: In Vitro Antioxidant Activity Of Gsh-slnmentioning

confidence: 99%

“…To evaluate the in vitro ability of such formulations to keep intact the antioxidant activity of GSH, the spectrophotometric DPPH assay was carried out [18][19][20]. DPPH radical has a deep violet colour in solution, and gradually it became pale yellow in the presence of non-encapsulated GSH.…”

Section: In Vitro Antioxidant Activity Of Gsh-slnmentioning

confidence: 99%

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Glutathione loaded solid lipid nanoparticles: Preparation and in vitro evaluation as delivery systems of the antioxidant peptide to immunocompetent fish cells

Trapani

Tripodo

Mandracchia

et al. 2016

JCB

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BACKGROUND: Solid lipid nanoparticles (SLN) have not been used for peptide supply to fish cells. OBJECTIVE: To evaluate the potential of SLN to deliver the antioxidant glutathione (GSH) to the primary cultures of head-kidney (HK) leucocytes of Sparus aurata L. METHODS: SLN were produced using the amphiphilic lipid Gelucire ® 50/13 according to the melt-emulsification method. In vitro stability and colorimetric studies for the antioxidant activity were carried out prior to biological assays on HK leucocytes. RESULTS: SLN sonicated in acidic medium were stable up to 3 months at 4 • C. A strong in vitro antioxidant activity effect for SLN was shown. The incubation of gilthead seabream HK leucocytes with GSH, GSSG or the sonicated and non-sonicated GSH-SLN (HAc) for 4 h had very few effects on HK cell activities. The phagocytic ability of HK leucocytes previously incubated with GSH was significantly increased in respect to the control cells. CONCLUSIONS:The technique of melt-emulsification provided particles with high association efficiency in GSH and even below 100 nm in size. However, no stimulant properties were observed after incubation of HK leucocytes with SLN and possible hypotheses explaining the observed findings are discussed.

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Section: In Vitro Antioxidant Activity Of Gsh-slnsupporting

confidence: 82%

Section: In Vitro Antioxidant Activity Of Gsh-slnmentioning

confidence: 99%

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Glutathione loaded solid lipid nanoparticles: Preparation and in vitro evaluation as delivery systems of the antioxidant peptide to immunocompetent fish cells

Trapani

Tripodo

Mandracchia

et al. 2016

JCB

Self Cite

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“…Полимерные наночастицы считаются одной из наиболее многообещающих стратегий в области наномедицины, поскольку обладают рядом преимуществ по сравнению с обычными препаратами, такими как контролируемое высвобождение лекарственного средства, повышенная растворимость, целевое поглощение лекарственного средства и биоразлагаемость. Полимерные наночастицы могут быть структурно различными, они могут состоять из: (а) однородной полимерной матрицы, диспергирующей или растворяющей активный ингредиент (наносферы или матричные системы); (б) масляного ядра, окруженного полимерной мембраной с лекарственным средством, растворенным в жидком ядре или адсорбированным на поверхности наночастиц, в зависимости от его физико-химических свойств (нанокапсул или систем резервуаров) [50]. Для приготовления этих наночастиц обычно используются природные или искусственные биоразлагаемые полимеры, и наиболее часто это поли-L-молочная кислота и сополимеры с гликолевой кислотой [50].…”

Section: идебенонunclassified

“…Полимерные наночастицы могут быть структурно различными, они могут состоять из: (а) однородной полимерной матрицы, диспергирующей или растворяющей активный ингредиент (наносферы или матричные системы); (б) масляного ядра, окруженного полимерной мембраной с лекарственным средством, растворенным в жидком ядре или адсорбированным на поверхности наночастиц, в зависимости от его физико-химических свойств (нанокапсул или систем резервуаров) [50]. Для приготовления этих наночастиц обычно используются природные или искусственные биоразлагаемые полимеры, и наиболее часто это поли-L-молочная кислота и сополимеры с гликолевой кислотой [50]. В клиническом исследовании оценивалась стабильность, раздражающее действие и антиоксидантная активность свободной формы идебенона по сравнению с наночастицами.…”

Section: идебенонunclassified

The role of mitochondrial dysfunction in Alzheimer’s disease

Коберская

2019

Medicinskij sovet

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Б олезнь Альцгеймера (БА)-распространенное нейродегенеративное заболевание, одна из самых частых причин развития когнитивных нарушений, достигающих степени деменции. Во всем мире каждые 7 секунд диагностируется новый случай БА, а во всем мире более 36,5 млн человек страдает БА [1]. По официальной статистике, у нас лишь около 9 тыс. пациентов с БА [2]. Большинство случаев БА списывают на естественные возрастные изменения («склероз», «маразм», «старческие причуды» и т.д.), и, по некоторым оценкам, порядка 90% российских случаев заболевания БА не диагностированы; пациенты с БА получают диагнозы хронической ишемии головного мозга, дисциркуляторной энцефалопатии, цереброваскулярной болезни [2]. В основе развития симптоматики при БА лежит отложение бета-амилоида с формированием внеклеточных сенильных бляшек и внутринейрональных включений тау-протеина, которое сопровождается нейрональной

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Bacteria-produced ferric exopolysaccharide nanoparticles as iron delivery system for truffles (Tuber borchii)

Picceri

Leonardi

Iotti

et al. 2017

Appl Microbiol Biotechnol

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Iron exopolysaccharide nanoparticles were biogenerated during ferric citrate fermentation by Klebsiella oxytoca DSM 29614. Before investigating their effects on Tuber borchii ("bianchetto" truffle) mycelium growth and morphology, they were tested on human K562 cell line and Lentinula edodes pure culture and shown to be non-toxic. Using these nanoparticles as iron supplement, the truffles showed extremely efficient iron uptake of over 300 times that of a commercial product. This avoided morphological changes in T. borchii due to lack of iron during growth and, with optimum nanoparticle dosage, increased growth without cell wall disruption or alteration of protoplasmatic hyphal content, the nuclei, mitochondria, and rough endoplasmic reticula being preserved. No significant modifications in gene expression were observed. These advantages derive from the completely different mechanism of iron delivery to mycelia compared to commercial iron supplements. The present data, in fact, show the nanoparticles attached to the cell wall, then penetrating it non-destructively without damage to cell membrane, mitochondria, chromatin, or ribosome. Low dosage significantly improved mycelium growth, without affecting hyphal morphology. Increases in hyphal diameter and septal distance indicated a healthier state of the mycelia compared to those grown in the absence of iron or with a commercial iron supplement. These positive effects were confirmed by measuring fungal biomass as mycelium dry weight, total protein, and ergosterol content. This "green" method for biogenerating iron exopolysaccharide nanoparticles offers many advantages, including significant economic savings, without toxic effects on the ectomycorrhizal fungus, opening the possibility of using them as iron supplements in truffle plantations.

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Chitosan Nanoparticles for Topical Co-administration of the Antioxidants Glutathione and Idebenone: Characterization and In vitro Release

Cited by 11 publications

References 24 publications

Glutathione loaded solid lipid nanoparticles: Preparation and in vitro evaluation as delivery systems of the antioxidant peptide to immunocompetent fish cells

Glutathione loaded solid lipid nanoparticles: Preparation and in vitro evaluation as delivery systems of the antioxidant peptide to immunocompetent fish cells

The role of mitochondrial dysfunction in Alzheimer’s disease

Bacteria-produced ferric exopolysaccharide nanoparticles as iron delivery system for truffles (Tuber borchii)

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