Chitosan oligosaccharides in combination with Agaricus blazei Murill extract reduces hepatoma formation in mice with severe combined immunodeficiency

Abstract

Chitosan and Agaricus blazei Murill (ABM) extracts possess antitumor activities. The aim of the present study was to investigate whether chitosan, ABM extract or the two in combination were effective against tumors in tumor-bearing mice. The mice were subcutaneously injected with SK-Hep 1 cells and were then were divided into the following six groups: Group 1, control group; group 2, chitosan 5 mg/kg/day; group 3, chitosan 20 mg/kg/day; group 4, ABM (246 mg/kg/day) and chitosan (5 mg/kg/day) combined; group 5, ABM (984 mg/kg/day) and chitosan (20 mg/kg/day) combined; and group 6, ABM (984 mg/kg/day). The mice were treated with the different concentrations of chitosan, ABM or combinations of the two for 6 weeks. The levels of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and vascular endothelial growth factor (VEGF), and tissue histopathological features were examined in the surviving animals. Based on the results of the investigation, the treatments performed in groups 2, 3 and 4 were identified as being capable of reducing the weights of the tumors, however, group 4, which was treated with chitosan (5 mg/kg/day) in combination with ABM (246 mg/kg/day) was able to reduce the levels of GOT and VEGF. As a result, treatment with chitosan in combination with ABM may offer potential in cancer therapy and requires further investigation.

Antitumor, Anti-inflammatory and Antiallergic Effects of Agaricus blazei Mushroom Extract and the Related Medicinal Basidiomycetes Mushrooms, Hericium erinaceus and Grifola frondosa: A Review of Preclinical and Clinical Studies

“…Wu et al [43,44] showed that AbM extract inhibited growth of colon cancer, hepatoma and melanoma in severe combined immunodeficient (SCID) mice and increased their life span in a dose-dependent manner. The finding of AbM (combined with chitosan N-acetyl glucosamine) inhibiting hepatoma cell growth in SCID mice was confirmed later [41]. Pinto et al [45] observed that a β-glucan-rich AbM preparation reduced growth of Ehrlich tumor after intratumor injection, increased blood levels of IFNγ, CD4+ T cells and macrophages (MΦ), and reduced interleukin (IL)-10, resulting in immune cell migration to tumor and cytokine switch.…”

Therapeutic Value of Medicinal Mushroom Agaricus blazei Murill

“…Agaricus blazei Murill (AbM) is a nutritive mushroom origin in Brazil. It is cultivated in countries like Indonesia, Taiwan, China, Japan and Korea 7 . It is a basidiomycete generally familiar as sun mushroom.…”

Agaricus blazei Murrill Polysaccharide Attenuates Periodontitis via H2S/NRF2 Axis‐Boosted Appropriate Level of Autophagy in PDLCs

“…Dosage Information/Dosage Regimen: All experimental procedures were approved by the Ethics Committee, West China School of Stomatology, Sichuan University (document number: WCHSIRB-D-2021-472). The experimental period was set to 14 days, and the dosage of ABMP was 1% (in drinking water w/v and daily intake of ABMP was 1100 mg kg -1 ) according to previous studies [32][33][34][35] and animal average daily water intake (Table S2, Supporting Information). In vitro, ABMP was administered at 5 μg mL -1 .…”