Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility

Abstract

In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds. Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%. Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity. Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds. A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.

Recent Trends in Alginate, Chitosan and Alginate-Chitosan Antimicrobial Systems

“…In the same time with increasing of calcium ions concentration the release rate of antimicrobial compound from systems decreases, because of this, systems containing the lowest concentration of calcium ions (5%), in the conditions of the experiment, showed shortest time in reducing of number of viable bacteria cells to zero [85]. In the alginate-chitosan sponges were encapsulated antimicrobial compounds like: silver sulfadiazine [83,86], gentamicin [84] and ciprofl oxacin [85]; in result, increased the antimicrobial activity against: Staphylococcus aureus [83][84][85][86], Pseudomonas aeruginosa [83,86] and Escherichia coli [85], compared to pristine alginate-chitosan sponges.…”

“…All of the studied alginate-chitosan antimicrobial sponges demonstrated sustained release of antimicrobial drugs from 4 hrs [85] to 30 days [84]. Due to these properties, alginate-chitosan antimicrobial sponges can be potentially used in wound dressing manufacturing [83,85,86] and in tissue engineering applications [84].…”

“…Alginate-chitosan sponge represent a tridimensional porous scaffold, prepared by freeze-drying technique of mixture [83] or consecutively deposited one above the other layers of alginate and chitosan [84][85][86]. Pore sizes range from <100 μm [85,86], 200-400 μm [83] and approximately 500 μm [84], as a function of various factors such as cross-linking time with Ca 2+ ions -with its increasing pore sizes being reduced [83] (Figure 8).…”

“…Pore sizes range from <100 μm [85,86], 200-400 μm [83] and approximately 500 μm [84], as a function of various factors such as cross-linking time with Ca 2+ ions -with its increasing pore sizes being reduced [83] (Figure 8). In the same time with increasing of calcium ions concentration the release rate of antimicrobial compound from systems decreases, because of this, systems containing the lowest concentration of calcium ions (5%), in the conditions of the experiment, showed shortest time in reducing of number of viable bacteria cells to zero [85].…”

“…Due to these properties, alginate-chitosan antimicrobial sponges can be potentially used in wound dressing manufacturing [83,85,86] and in tissue engineering applications [84].…”