Chitosan-whey protein nanoparticles improve encapsulation efficiency and stability of a trypsin inhibitor isolated from Tamarindus indica L

Queiroz, Jaluza Luana Carvalho de; Costa, Rafael Oliveira de Araújo; Matias, Lídia Leonize Rodrigues; Medeiros, Amanda Fernandes de; Gomes, Ana Francisca Teixeira; Pais, Tatiana dos Santos; Passos, Thaís Souza; Maciel, Bruna Leal Lima; Santos, Elisama Vieira dos; Morais, Ana Heloneida de Araújo

doi:10.1016/j.foodhyd.2018.06.010

Cited by 41 publications

(37 citation statements)

References 30 publications

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“…Empty nanoparticles (CW) without TTI were also prepared, using a 1:1 ( w / w ) ratio of encapsulating agents. The chitosan purification step was performed based on Kumari et al [21] with modifications proposed by Queiroz et al [16].…”

Section: Methodsmentioning

confidence: 99%

“…Both nanoformulations (ECW and CW) were characterized by scanning electron microscopy (SEM), laser diffraction (LD), and Fourier transform infrared spectroscopy (FTIR), according to Queiroz et al [16], with few modifications in LD. To measure the diameter and the polydispersity index (PI), the dispersions containing the nanoparticles, previously crosslinked in triplicate, were filtered, and the precipitates were dispersed in 4 mL of water and analyzed in a NanoBrookZetaPlus Zeta Potential Analyzer.…”

Section: Methodsmentioning

confidence: 99%

“…The incorporation efficiency of TTI was determined to measure antitrypsin activity according to the methodology proposed by Kakade [20] and based on Queiroz et al [16].…”

Section: Methodsmentioning

confidence: 99%

“…Thus, alternatives to potentiate the effect of TTI are needed.The encapsulation technique can improve the bioactive action, once nanoparticles (<100 nm) with uniform particle size distribution can improve the bioactive properties and promote the controlled release of bioactive molecules [15]. Queiroz et al [16] observed that the combination ofchitosan–whey protein was essential to protect TTI and guarantee better inhibitory activity results against trypsin. Additionally, Costa [17] revealed that the same nanoformulation did not show signs of toxicity in vitro and in vivo, suggesting a safe application of these particles.…”

Section: Introductionmentioning

confidence: 99%

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Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles Reduces Fasting Blood Glucose Levels without Compromising Insulinemia: A Preclinical Study

et al. 2019

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In vivo studies show the benefits of the trypsin inhibitor isolated from tamarind (Tamarindusindica L.) (TTI) seeds in satiety and obesity. In the present study, TTI nanoencapsulation (ECW) was performed to potentialize the effect of TTI and allow a controlled release in the stomach. The impact on glycemia, insulin, and lipid profile was evaluated in Wistar rats overfed with a high glycemic index diet (HGLI). Characterization of the nanoparticles and in vitro stability in simulated gastrointestinal conditions, monitored by antitrypsin activity and HPLC, was performed. ECW and empty nanoparticles (CW) were administered by gavage, using 12.5 and 10.0 mg/kg, respectively. Both nanoformulations presented a spherical shape and smooth surface, with an average diameter of 117.4 nm (24.1) for ECW and 123.9 nm (11.3) for CW. ECW maintained the antitrypsin activity (95.5%) in the gastric phase, while TTI was completely hydrolyzed. In Wistar rats, the nanoformulations significantly reduced glycemia and HOMA IR, and ECW increased HDL-c compared to CW (p < 0.05).Pancreas histopathology of animals treated with ECW suggested an onset of tissue repair. Thenanoencapsulation provided TTI protection, gradual release in the desired condition, and improvement of biochemical parameters related to carbohydrate metabolism disorders,without compromising insulinemia.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The incorporation efficiency of TTI was determined to measure antitrypsin activity according to the methodology proposed by Kakade [20] and based on Queiroz et al [16].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles Reduces Fasting Blood Glucose Levels without Compromising Insulinemia: A Preclinical Study

et al. 2019

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“…The combination of the encapsulating agents showed excellent incorporation efficiency and TTI stability. In addition, encapsulation in chitosan and whey protein promoted the reduction of the half maximal inhibitory concentration (IC50) and preserved inhibitory activity at temperatures up to 80 °C compared to isolated agents, showing that encapsulation is an interesting strategy to improve the function and stability of TTI 98 .…”

Section: Trypsin Inhibitors In the Treatment Of Chronic Diseases: Chamentioning

confidence: 99%

Trypsin inhibitors: promising candidate satietogenic proteins as complementary treatment for obesity and metabolic disorders?

Lima

Piuvezam

Maciel

et al. 2019

Journal of Enzyme Inhibition and Medicinal Chemistry

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The increase in non-communicable chronic diseases has aroused interest in the research of adjuvants to the classic forms of treatments. Obesity and metabolic syndrome are the main targets of confrontation because they relate directly to other chronic diseases. In this context, trypsin inhibitors, molecules with wide heterologous application, appear as possibilities in the treatment of overweight and obesity due to the action on satiety related mechanisms, mainly in the modulation of satiety hormones, such as cholecystokinin. In addition, trypsin inhibitors have the ability to also act on some biochemical parameters related to these diseases, thus, emerging as potential candidates and promising molecules in the treatment of the obesity and metabolic syndrome. Thus, the present article proposes to approach, through a systematic literature review, the advantages, disadvantages and viabilities for the use of trypsin inhibitors directed to the treatment of overweight and obesity.

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Synbiotic Encapsulation with Maillard Reaction Product and Okra Pectic Oligosaccharide to Enhance the Survival of Lactiplantibacillus Plantarum

Yeung¹,

Kang²,

Chang³

2023

Food Bioprocess Technol

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Chitosan-whey protein nanoparticles improve encapsulation efficiency and stability of a trypsin inhibitor isolated from Tamarindus indica L

Cited by 41 publications

References 30 publications

Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles Reduces Fasting Blood Glucose Levels without Compromising Insulinemia: A Preclinical Study

Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles Reduces Fasting Blood Glucose Levels without Compromising Insulinemia: A Preclinical Study

Trypsin inhibitors: promising candidate satietogenic proteins as complementary treatment for obesity and metabolic disorders?

Synbiotic Encapsulation with Maillard Reaction Product and Okra Pectic Oligosaccharide to Enhance the Survival of Lactiplantibacillus Plantarum

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