Chitosanase activity inBacillus thuringiensis

Camarillo, Rafael; Pérez, O. Sánchez; Avelizapa, N.G Rojas; Ramírez, Manrique; Avelizapa, Luz Irene Rojas

doi:10.1007/bf02931653

Cited by 14 publications

(9 citation statements)

References 20 publications

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“…Hence, the production of COS using glycolytic enzymes is more efficient and desirable. Accordingly, chitosanases have been isolated from bacteria (Choi et al , 2004; Cruz Camarillo et al , 2004; Yun et al , 2005; Su et al , 2006), fungi (Nogawa et al , 1998; Cheng & Li, 2000; Zhang et al , 2000) and plants (Osswald et al , 1994), with a view to using them in the production of COS.…”

Section: Introductionmentioning

confidence: 99%

Identification and expression of GH-8 family chitosanases from severalBacillus thuringiensissubspecies

Lee

Jang

Choi

et al. 2007

FEMS Microbiology Letters

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The Gram-positive spore-forming bacterium, Bacillus thuringiensis, a member of the Bacillus cereus group, produces chitosanases that catalyze the hydrolysis of chitosan to chitosan-oligosaccharides (COS). Although fungal and bacterial chitosanases belonging to other glycoside hydrolase (GH) families have been characterized in a variety of microorganisms, knowledge on the genetics and phylogeny of the GH-8 chitosanases remains limited. Nine genes encoding chitosanases were cloned from 29 different serovar strains of B. thuringiensis and they were expressed in Escherichia coli. The ORFs of the chitosanases contained 1,359 nucleotides and the protein products had high levels of sequence identity (>96%) to other Bacillus species GH-8 chitosanases. Thin-layer chromatography and HPLC analyses demonstrated that these enzymes hydrolyzed chitosan to a chitosan-trimer and a chitosan-tetramer as major products, and this could be useful in the production of COS. In addition, a simple plate assay was developed, involving a soluble chitosan, for high-throughput screening of chitosanases. This system allowed screening for mutant enzymes with higher enzyme activity generated by error-prone PCR, indicating that it can be used for directed chitosanase evolution.

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Section: Introductionmentioning

confidence: 99%

Identification and expression of GH-8 family chitosanases from severalBacillus thuringiensissubspecies

Lee

Jang

Choi

et al. 2007

FEMS Microbiology Letters

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“…Colloidal chitosan was prepared based on the method adopted by Cruz Camarillo et al, (2003) [4] with slight modification. Crude chitosan flake was procured from MATSYAFED, Kerala State Co-operative Federation for Fisheries Development Ltd. Neendakara, Kerala.…”

Section: Synthesis Of Colloidal Chitosanmentioning

confidence: 99%

Synthesis of colloidal chitosan from sea waste and its antifeedant effect against maize fall armyworm Spodoptera frugiperda (J.E. Smith)

Moorthy¹,

Shanthi²,

Chinniah³

et al. 2021

J. Entomol. Zool. Stud.

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Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) is one of the most important pests of maize, suddenly appeared in India 2018 and other cereal crops. The colloidal chitosan was synthesized in the laboratory, from the waste of sea food and studied for its bio efficacy by leaf dip bioassay on FAW. The antifeedant effect was assessed on first instar larva at concentration of one, three, five and seven per cent under no choice condition. The one and 7 per cent colloidal chitosan showed 40.51 per cent and 85.38 per cent antifeedant effect over untreated check respectively. The leaf weight loss was observed at one and 7 per cent colloidal chitosan exhibited 24.95 and 11.05 per cent respectively. The per cent reduction in leaf consumption on weight basis was 66.68 per cent when fed with 7 per cent colloidal chitosan treated leaf. Among different concentration used 7 per cent colloidal chitosan showed higher antifeedant effect against first instar larva of FAW.

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“…The absence of bands with MW close to 70,000 indicated that most of chitosanolytic activity resides in a minority protein. The molecular mass of chitosanases from various B. thuringiensis strains is in the range between 46-59 kDa [31,32], as occurs in chitosanases from related bacterial species such as B. cereus [42] and B. megaterium [43], but higher than in other Bacillus species (20-30 kDa) [44]. In contrast, chitinases from B. thuringiensis are slightly larger (66-75 kDa) [27,45].…”

Section: Isolation and Characterization Of B Thuringiensis Chitosanolytic Activitymentioning

confidence: 99%

“…It also displays proteinase, chitinase [27][28][29] and chitosanase [30] activities, which are probably involved in its ecological interactions as well as in the insecticidal and antifungal action, and could expand its range of applications [18]. Cruz-Camarillo et al first described a chitosanase activity in various strains of B. thuringiensis [31]. Lee et al reported the isolation of several of these enzymes [32].…”

Section: Introductionmentioning

confidence: 99%

Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis

Santos-Moriano

Kidibule

Alleyne

et al. 2018

Process Biochemistry

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Starting from a commercial preparation of Bacillus thuringiensis var. aizawai, widely employed as biopesticide in agriculture, we obtained a chitosanolytic activity by washing the cells with concentrated sodium acetate. The optimum pH and temperature values for this enzyme were 6.0 and 60 °C, respectively. The enzymatic extract efficiently hydrolyzed various chitosans with different deacetylation degrees (DD) into small oligosaccharides. Combining mass spectrometry (ESI-Q-TOF) and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), we observed the formation of fully deacetylated chitooligosaccharides [fdCOS, (GlcN)2-5] and, to a lesser extent, partially acetylated chitooligosaccharides [paCOS, GlcNAc-(GlcN)1-3]. A 10 g/L solution of chitosan with 600-800 kDa and DD ≥ 90% was fully converted into oligosaccharides in 55 h. Most of the products were fdCOS: 1.6 g/L of chitobiose, 1.7 g/L of chitotriose, 5 g/L of chitotetraose and 1.4 g/L of chitopentaose.

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Chitosanase activity inBacillus thuringiensis

Cited by 14 publications

References 20 publications

Identification and expression of GH-8 family chitosanases from severalBacillus thuringiensissubspecies

Identification and expression of GH-8 family chitosanases from severalBacillus thuringiensissubspecies

Synthesis of colloidal chitosan from sea waste and its antifeedant effect against maize fall armyworm Spodoptera frugiperda (J.E. Smith)

Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis

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