Chl a fluorescence and proteomics reveal protection of the photosynthetic apparatus to dehydration in tolerant but not in susceptible wheat cultivars

Nykiel, Małgorzata; Lisik, Piotr; Dębski, Janusz; Florea, Bogdan I.; Rybka, Krystyna

doi:10.32615/bp.2019.033

Cited by 3 publications

(3 citation statements)

References 57 publications

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“…Three gels, one from each independent biological replication, were used for the identification of differential proteins. The gels were stained with 0.1% (w/v) Coomassie brilliant blue R-250 (Sigma-Aldrich Ltd., Pozna ń, Poland) overnight, destained, and stored in 5% acetic acid at 4 • C for further analysis [78].…”

Section: First-dimensionmentioning

confidence: 99%

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Aluminum Stress Induces Irreversible Proteomic Changes in the Roots of the Sensitive but Not the Tolerant Genotype of Triticale Seedlings

Niedziela

Domżalska

Dynkowska

et al. 2022

Plants

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Triticale is a wheat–rye hybrid with a higher abiotic stress tolerance than wheat and is better adapted for cultivation in light-type soils, where aluminum ions are present as Al-complexes that are harmful to plants. The roots are the first plant organs to contact these ions and the inhibition of root growth is one of the first plant reactions. The proteomes of the root apices in Al-tolerant and -sensitive plants were investigated to compare their regeneration effects following stress. The materials used in this study consisted of seedlings of three triticale lines differing in Al3+ tolerance, first subjected to aluminum ion stress and then recovered. Two-dimensional electrophoresis (2-DE) was used for seedling root protein separation followed by differential spot analysis using liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS/MS). The plants’ tolerance to the stress was evaluated based on biometric screening of seedling root regrowth upon regeneration. Our results suggest that the Al-tolerant genotype can recover, without differentiation of proteome profiles, after stress relief, contrary to Al-sensitive genotypes that maintain the proteome modifications caused by unfavorable environments.

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Section: First-dimensionmentioning

confidence: 99%

“…The taxonomic category selected was Triticum aestivum. Only peptides passing a Mascot-defined expectation value of 0.05 were considered as positive identifications [78]. The functional networks of differentially expressed proteins were constructed using the STRING database [32].…”

Section: Protein Identification By Mass Spectrometry and Database Searchmentioning

confidence: 99%

Aluminum Stress Induces Irreversible Proteomic Changes in the Roots of the Sensitive but Not the Tolerant Genotype of Triticale Seedlings

Niedziela

Domżalska

Dynkowska

et al. 2022

Plants

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show abstract

“…Protein is one of the most basic components of life activities and is one of the important components of body cells, which is essential for the body's physiological functions [5][6]. Wheat proteins, however, have high nutritional value, good quality properties and important preparative properties, which are very important in wheat research [7][8]. Therefore, the study of wheat protein fractions is particularly urgent.…”

Section: Introductionmentioning

confidence: 99%

Fourier transform-based protein fraction analysis of whole-seed wheat

Jia,

Xie,

Chang

2023

Applied Mathematics and Nonlinear Sciences

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The principle of the Fourier transform is explored in this paper to compare protein structure similarity, transforming protein structure into a distance sequence, and performing spectral analysis on a fast Fourier transform. Secondly, protein fraction classification and similarity analysis of whole-seed wheat protein fractions were performed using the fast Fourier transform. Fourier transform infrared spectra were analyzed using two parameters: diatomic vibrations and molecular leaps. Finally, the whole seed wheat protein fraction content test analysis was analyzed experimentally. The results showed that the spectral range of whole seed wheat protein fractions was selected from 10539 cm −1 to 6080 cm −1, the fraction of fractions was determined as 9, the parameter R 2 val was 0.9614. This paper provides practical reference material for researching whole-seed wheat protein fields.

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Turf covering for sport season elongation cause no stress for grass species as detected by Chl a fluorescence

Prokopiuk

Żurek

Rybka

2019

Urban Forestry & Urban Greening

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Chl a fluorescence and proteomics reveal protection of the photosynthetic apparatus to dehydration in tolerant but not in susceptible wheat cultivars

Abstract: This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence

Cited by 3 publications

References 57 publications

Aluminum Stress Induces Irreversible Proteomic Changes in the Roots of the Sensitive but Not the Tolerant Genotype of Triticale Seedlings

Aluminum Stress Induces Irreversible Proteomic Changes in the Roots of the Sensitive but Not the Tolerant Genotype of Triticale Seedlings

Fourier transform-based protein fraction analysis of whole-seed wheat

Turf covering for sport season elongation cause no stress for grass species as detected by Chl a fluorescence

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