Chlorogenic Acid Functions as a Novel Agonist of PPAR<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>γ</mml:mi></mml:mrow></mml:math>2 during the Differentiation of Mouse 3T3-L1 Preadipocytes

Peng, Shuguang; Pang, Yilin; Zhu, Qi; Kang, Jian; Liu, Mingxin; Wang, Zheng

doi:10.1155/2018/8594767

Cited by 26 publications

(14 citation statements)

References 70 publications

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“…It is known that CD36/SR-B2 expression is regulated by agonists of the nuclear peroxisome proliferator-activated receptors (PPAR). Recent studies performed on 3T3L1 preadipocytes identified chlorogenic acid as PPARγ2 (20 µM) and PPARα (50 µM) agonist [46,47]. Our results revealed that V. opulus did not influence the expression of the gene encoding PPARα allowing us to suspect that other V. opulus polyphenolic compounds may act as PPAR or CD36/SR-B2 antagonist.…”

Section: Resultsmentioning

confidence: 46%

Viburnum opulus Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells

et al. 2019

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In recent years, there has been increasing interest in studying food-originated phytocompounds with beneficial influences for humans. Amongst the most active natural substances are polyphenols, for which high content has been identified in the Viburnum opulus berry, and which are unused in Western Europe. Due to its strong antioxidant activity we explored the potential of V. opulus as a preventive agent against diet-related chronic diseases, such as obesity and type 2 diabetes. Among the causes of these ailments is oxidative stress, as well as impaired glucose and free fatty acids (FFA) uptake. Thus, the purpose of this study was to determine biological activity of V. opulus phenolic extracts as cytoprotective agents able to decrease induced oxidative stress, lower lipid accumulation and attenuate glucose and FFA uptake by Caco-2 cells via GLUT2 and CD36/FAT transporters. To determine the source of the most biologically active phenolic compounds, we obtained four phenolic compounds extracts as crude juice, phenolics isolated from juice and two preparations of phenolics obtained with different extraction agents from fruit pomace. Among the studied extracts, the phenolic rich fraction obtained from fruit juice revealed the strongest activity to decrease uptake of glucose, FFA and accumulation of lipid droplets in Caco-2 cells without affecting their viability (IC0 50 μg/mL). Observed uptake attenuation was followed by decrease of the CD36/FAT gene expression, without influence on the GLUT2 and PPARα levels. We suspect that V. opulus phenolics were able to modulate the cellular membrane dynamic, although that hypothesis requires further, more detailed studies. Extracts revealed strong chemo-preventive activity against oxidative stress induced chemically by tert-butylhydroperoxide (t-BOOH), as well as against DNA damage through the induction of DNA repair after cell exposition to methylnitronitrosoguanidine (MNNG) and H2O2. Our findings suggest Viburnum opulus fruit as a dietary source of phytocompounds, which could be considered as a tailored design food supplement components for the prevention and treatment of postprandial elevation of glucose and fatty acids through delaying the rate of glucose and fatty acid absorption by intestinal cells.

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Section: Resultsmentioning

confidence: 46%

Viburnum opulus Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells

et al. 2019

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“…They were able to down-regulate the expression of adipogenic transcription factors (PPARγ and C/EBP) and adipocyte-specific proteins (leptin), as well as up-regulate adiponectin. In comparison, other study performed with 3T3-L1 differentiated cells identified chlorogenic acid as an agonist of PPARγ2, which promoted adipocyte differentiation via the elevation of PPARγ2, CEBP and SREBP-1 mRNA and protein level [ 54 ]. Nevertheless, the other phenolic compounds identified in V. opulus fruit were proved to decrease the adipogenesis process [ 55 , 56 , 57 ].…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, PPARγ transcriptional potential could be modulated on different levels: by the regulation of mRNA and protein levels, as well as conformational changes made after its binding with ligands leading to protein subcellular distribution, heterodimers’ formation, and, finally, binding with peroxisome proliferator hormone response elements present in promoters of PPAR-responsive genes. The studies performed recently identified chlorogenic acid as an agonist of the PPARγ2 receptor responsible for the enhanced differentiation of 3T3-L1 cells [ 54 ]. In the identified molecular mechanism downregulation of adipocyte differentiation-inhibitor gene, Pref1 was observed, which was accompanied by the upregulation of CEBP and SREBP-1 transcriptional factors.…”

Section: Resultsmentioning

confidence: 99%

Viburnum opulus L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity

et al. 2020

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Viburnum opulus L. fruit is a rich source of phenolic compounds that may be involved in the prevention of metabolic diseases. The purpose of this study was to determine the effects of Viburnum opulus fresh juice (FJ) and juice purified by solid-phase extraction (PJ) on the adipogenesis process with murine 3T3-L1 preadipocyte cell line and pancreatic lipase activity in triolein emulsion, as well as their phenolic profiles by UPLC/Q-TOF-MS. Decrease of lipids and triacylglycerol accumulation in differentiated 3T3-L1 cells were in concordance with downregulation of the expression of peroxisome proliferator-activated receptor-gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPβ/α), and sterol regulatory element-binding protein 1c (SREBP-1c). Furthermore, regulation of PPARγ-mediated β-lactamase expression by V. opulus components in reporter gene assay, as well as their binding affinity to ligand-binding domain of PPARγ, were tested. In addition, the levels of enzymes involved in lipid metabolism, like fatty acid synthase (FAS) or acetyl-CoA carboxylase (ACC), were decreased, along with inflammatory cytokines, like tumor necrosis factorα (TNFα), interleukin-6 (Il-6) and leptin. Moreover, FJ and PJ were able to inhibit pancreatic lipase, which potentially could reduce the fat absorption from the intestinal lumen and the storage of body fat in the adipose tissues. Thirty-two phenolic compounds with chlorogenic acid as the dominant compound were identified in PJ which revealed significant biological activity. These data contribute to elucidate V. opulus juice phenolic compounds’ molecular mechanism in adipogenesis regulation in 3T3-L1 cells and dietary fat lipolysis.

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“…Vanillic acid presented a strong effect reducing TAG content in 3T3-L1 adipocytes through the reduction of AdipoQ , Plin1 , and Fabp4 expression [64]. Chlorogenic acid has shown to increase lipolysis and up-regulate the expression of lipases ( Hsl and Dgat1 ) [65,66]. Similarly, caffeine has exhibited potential to up-regulate HSL and AGTL and increase lipolysis, reducing TAG content in 3T3-L1 cells [67,68].…”

Section: Resultsmentioning

confidence: 99%

Relationship of the Phytochemicals from Coffee and Cocoa By-Products with their Potential to Modulate Biomarkers of Metabolic Syndrome In Vitro

et al. 2019

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This study aimed to compare the phytochemicals from coffee and cocoa by-products and their relationship with the potential for reducing markers of inflammation, oxidative stress, adipogenesis, and insulin resistance in vitro. We characterized the phytochemical profile of extracts from coffee husk, coffee silverskin, and cocoa shell and evaluated their in vitro biological activity in RAW264.7 macrophages and 3T3-L1 adipocytes. Pearson correlations and principal component regressions were performed to find the contribution of phytochemicals and underlying mechanisms of action. Coffee husk and silverskin extracts were mainly composed of caffeine and chlorogenic acid. Major components in cocoa shell included theobromine and protocatechuic acid. Both coffee and cocoa by-product extracts effectively reduced inflammatory markers in macrophages and adipocytes (NO, PGE2, TNF-α, MCP-1, and IL-6) and the production of reactive oxygen species (21.5–66.4%). Protocatechuic and chlorogenic acids, together with caffeine, were suggested as main contributors against inflammation and oxidative stress. Furthermore, extracts reduced lipid accumulation (4.1–49.1%) in adipocytes by regulating lipolysis and inducing adipocyte browning. Gallic and chlorogenic acids were associated with reduced adipogenesis, and caffeine with adipocyte browning. Extracts from coffee and cocoa by-products also modulated the phosphorylation of insulin receptor signaling pathway and stimulated GLUT-4 translocation (52.4–72.9%), increasing glucose uptake. The insulin-sensitizing potential of the extracts was mainly associated with protocatechuic acid. For the first time, we identified the phytochemicals from coffee and cocoa by-products and offered new insights into their associations with biomarkers of inflammation, oxidative stress, adipogenesis, and insulin resistance in vitro.

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Chlorogenic Acid Functions as a Novel Agonist of PPARγ2 during the Differentiation of Mouse 3T3-L1 Preadipocytes

Cited by 26 publications

References 70 publications

Viburnum opulus Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells

Viburnum opulus Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells

Viburnum opulus L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity

Relationship of the Phytochemicals from Coffee and Cocoa By-Products with their Potential to Modulate Biomarkers of Metabolic Syndrome In Vitro

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