Chlorophyll fluorescence as a bioindicator of effects on growth in aquatic macrophytes from mixtures of polycyclic aromatic hydrocarbons

Marwood, Christopher A.; Solomon, Keith R.; Greenberg, Bruce M.

doi:10.1002/etc.5620200425

Cited by 128 publications

(95 citation statements)

References 45 publications

(69 reference statements)

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“…Among the parameters used to describe fluorescence of chl, initial fluorescence of Chl (F 0 ), potential yield of photochemical reactions in PSII (F v /F m ), and effective quantum yield of PSII (ϕ PSII ) are used most frequently in studying the stress physiology of plants (Krause and Weis, 1991;Marwood et al, 2001;Mallick and Mohn, 2003;Kummerová and Vanová, 2007). In the present study, F 0 was greater when soybeans were exposed to Cd or FLT.…”

Section: Discussionmentioning

confidence: 99%

“…Because PSII is the first step in photosynthetic electron transport, inhibition of electron transport anywhere within the electron transport chain exerts excitation pressure on PSII. Changes in PSII and ϕ PSII indicate a greater proportion of inactive PSII reaction centers due to inhibition of photochemical processes of photosynthesis related to electron transport chain in the thylakoid membrane, such as oxidation or degradation of D1 proteins, impairment of PSI leading block of the downstream electron transport, and severely reduced pigment concentrations in reaction centers (Anderson et al, 1997;Huang et al, 1997;Marwood et al, 2001). The results of the current study indicated that exposure to Cd or FLT resulted in less absorption of light energy, electron transport, conversion of light energy and photo phosphorylation in soybean seedlings.…”

Section: Discussionmentioning

confidence: 99%

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Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings

Shi

et al. 2013

Journal of Environmental Sciences

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The single and combinational effects of cadmium (Cd) and fluoranthene (FLT) on germination, growth and photosynthesis of soybean seedlings were investigated. Exposure to 5, 10, or 15 mg Cd/L or 1, 5, or 10 mg FLT/L individually or in combination significantly decreased germination vigor (3 days) and final germination rate of soybean seeds, except at 1 and 5 mg FLT/L. The results of two-way ANOVA analysis and the Bliss independence model showed that at lower concentrations of FLT (1 mg/L), the interaction between Cd and FLT on germination was antagonistic, whereas the interaction was synergistic when the concentration of FLT was 5 or 10 mg/L and the concentration of Cd was 15 mg/L. Growth, expressed as dry weight, length of shoot and root, leaf area, and photosynthesis, expressed as net photosynthetic rate, intercellular CO 2 concentration, chlorophyll contents and fluorescence of soybean seedlings were also reduced by exposure to 5 or 10 mg Cd/L or 1 or 5 mg FLT/L, singly or jointly. Significant antagonistic effects of exposure to 5 or 10 mg Cd/L or 1 or 5 mg FLT/L on shoot growth and photosynthesis were observed, whereas synergy and antagonism of Cd and FLT were both observed for root growth.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings

Shi

et al. 2013

Journal of Environmental Sciences

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“…The principles of this method are described in [38][39][40]. The quantum yield of the PSII correlates with photosynthetic carbon assimilation [38] and indicates inhibition of photosynthetic activity by a stressor [41,42]. For these measurements, the test organisms had to be taken off the medium.…”

Section: Measurements Of the Quantum Yieldmentioning

confidence: 99%

Impact of heterophylly on the sensitivity of Myriophyllum aquaticum biotests

Ebke¹,

Felten

Dören³

2013

Environ Sci Eur

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Background: Parrot feather (Myriophyllum aquaticum) is an amphibious macrophyte with an inducible heterophylly. M. aquaticum plants adapted to the emersed state have leaves with a distinct cuticle and water repellent properties. In contrast M. aquaticum plants adapted to the submerged state have leaves typical for submerged hydrophytes with a strongly reduced cuticle. The aim of the study was to evaluate if this heterophylly of M. aquaticum affects the results of macrophyte biotests. Therefore, the two model substances atrazine and 2,4-dichlorophenoxyacetic acid (2,4-D) were tested each with three M. aquaticum biotests, the only modified parameter being the adaptation time to submergence (0, 7, 28 days). Results: Root length was the most sensitive growth endpoint regarding the test substances atrazine and 2,4-D. Biotests with plants adapted to the submerged state show three times more sensitive results (EC50 7d 142.2 μg/L, EC50 28d 154.5 μg/L) than biotests with plants without an adaptation phase (EC50 0d 458.8 μg/L) in case of atrazine and five times more sensitive results (EC50 28d 46.9 μg/L, EC50 0d 246.3 μg/L) in case of 2,4-D. Apart from the differences in sensitivity, the differently adapted M. aquaticum plants show a completely different growth behavior. The growth rates based on shoot length were nearly ten times higher in the biotests with not adapted M. aquaticum plants than in the biotests with plants 28 days adapted to submergence. Additional measurements of the quantum yield of PSII could demonstrate that rapid growth in length is not based on photosynthetic carbon assimilation. Conclusions: The heterophylly of M. aquaticum affects significantly the sensitivity of aquatic macrophyte biotests and should be taken into account in the development of a standardized test design. The 2,4-D results show the importance of an additional macrophyte biotest to the Lemna test, where the no observed effect concentration (NOEC) value is more than 30 times lower (7 μg/L) than the NOEC value of Lemna in the literature (270 μg/L). Furthermore, the growth rate endpoint in macrophyte biotests should not be misinterpreted. Rapid shoot elongation of amphibious macrophytes, which become submerged, is mainly caused by ethylene-triggered endogenous processes that are not connected to photosynthetic carbon assimilation and appear to be part of a stress reaction to avoid adverse environmental conditions.

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“…In plants, they cause oxidative stress with a high level of generation of Reactive Oxygen Species (ROS), cell death, and necrosis (Burritt, 2008;Paskova et al, 2006). They affect growth by reducing photosynthesis activity, transpiration rate, and nutrient uptake (Liu et al, 2009;Marwood et al, 2001;Redondo-Gómez et al, 2011;Witting et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

The halophytic model plant Thellungiella salsuginea exhibited increased tolerance to phenanthrene-induced stress in comparison with the glycophitic one Arabidopsis thaliana: Application for phytoremediation

Shiri

Rabhi

Abdelly

et al. 2015

Ecological Engineering

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Polycyclic aromatic hydrocarbons constitute a large family of organic environmental pollutants. Hence, a particular attention has been attributed to all approaches involved in the reduction of their contamination in water and natural ecosystems. Plant tolerance, absorption, accumulation, and likely biodegradation of these pollutants, known as phytoremediation, have emerged as an efficient technique to remediate environments contaminated with polycyclic aromatic hydrocarbons. The present work was aimed to compare the tolerance to phenanthrene (a polycyclic aromatic hydrocarbon) in the two model plants: Arabidopsis thaliana (glycophyte) and Thellungiella salsuginea (halophyte). Our study showed that the development of these two species was reduced under phenanthrene stress, the effect being more pronounced in Arabidopsis thaliana than in Thellungiella salsuginea. In parallel, results from the intrinsic quantum yield of photosystem II and chlorophyll concentrations were concomitant with those of growth and phenotypic changes, and confirmed the higher tolerance of Thellungiella salsuginea compared to that of Arabidopsis thaliana. The intrinsic quantum yield of photosystem II was drastically decreased in the glycophyte, which indicates a marked disturbance in photosystem II performance. This induced a severe oxidative stress as shown by the utilization of specific reactive oxygen species probes. In parallel, the activities of glutathione reductase, gaiacol peroxidase, and superoxide dismutase were increased by 95, 73, and 36%, respectively, which indicates a marked phenanthrene-induced oxidative stress. In Thellungiella salsuginea, photosystem II performance was not significantly affected. This species showed less accumulated reactive oxygen species than Arabidopsis thaliana. Its enzymatic antioxidant system showed few changes as superoxide dismutase was the only enzyme whose activity was enhanced (+34%). A 3 much higher capacity of recovery was also noticed in this halophyte as compared to the glycophyte. Indeed, it seems that Thellungiella salsuginea accumulated phenanthrene in stomata, which suggests its possible volatilization. All these data, taken together, add new insight to the mechanisms involved in halophytic plant tolerance to abiotic stresses and their potential use in phytoremediation. Graphical abstract Phenanthrene Glycophytic

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Chlorophyll fluorescence as a bioindicator of effects on growth in aquatic macrophytes from mixtures of polycyclic aromatic hydrocarbons

Cited by 128 publications

References 45 publications

Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings

Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings

Impact of heterophylly on the sensitivity of Myriophyllum aquaticum biotests

The halophytic model plant Thellungiella salsuginea exhibited increased tolerance to phenanthrene-induced stress in comparison with the glycophitic one Arabidopsis thaliana: Application for phytoremediation

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