Chlorophyll fluorescence imaging to facilitate breeding of Bremia lactucae-resistant lettuce cultivars

Bauriegel, Elke; Brabandt, H.; Gärber, Ute; Herppich, Werner B.

doi:10.1016/j.compag.2014.04.010

Cited by 37 publications

(18 citation statements)

References 26 publications

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“…These health benefits have been linked to a range of micro-and macro-nutrients, Sensitivity to salt stress differs with the various stages of a plant's development [9,17], with stronger responses during reproductive stages than in early growth [17]. In lettuce, several studies have been performed using chlorophyll fluorescence (CF) to investigate the effect of a range of potentially stressful factors, such as light quality [7] and disease resistance [18], on outcomes such as post-harvest quality [19][20][21]. In addition, the effects of NaCl concentrations on growth and nutritional parameters have also been examined in multiple varieties of lettuce [6,8,19,22,23].…”

Section: Introductionmentioning

confidence: 99%

Response to Salt Stress in Lettuce: Changes in Chlorophyll Fluorescence Parameters, Phytochemical Contents, and Antioxidant Activities

et al. 2020

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Chlorophyll fluorescence (CF), growth parameters, phytochemical contents [proline, chlorophyll, ascorbic acid, total phenol content (TPC), total flavonoid content (TFC)], and antioxidant activities were investigated in lettuce (Lactuca sativa L.) seedlings grown under different sodium chloride (NaCl) concentrations (0, 50, 100, 200, 300, and 400 mM) in a controlled environment for eight days. The parameters were evaluated at two days intervals. Almost of the CF and growth parameters as well as phytochemicals were significantly affected by both NaCl concentrations and progressive treatment schedule. The maximum quantum yield (Fv/Fm), effective quantum yield of photochemical energy conversion in PSII [Y(PSII)], coefficient of photochemical quenching (qP), coefficient of non-photochemical quenching (qN), and ratio of fluorescence decline (Rfd) showed decrements only at the highest saline concentration (400 mM), whereas the quantum yield of non-regulated energy dissipation in PSII [Y(NO)] exhibited a dissipation trend. All the growth parameters decreased with increasing NaCl concentrations, showing the highest decrease (~8 fold) in shoot fresh weight, compared to control seedlings. Proline significantly increased with increasing NaCl concentration and treatment time. Other phytochemicals decreased with the increase in NaCl concentration and reached their lowest at 400 mM. Overall, the results showed major changes in all parameters when the seedlings were grown at a NaCl concentration of 400 mM. The present findings will be useful for understanding the differential effect of NaCl concentrations in lettuce seedlings, and also might be useful to optimize the NaCl concentrations in other crops grown in controlled environmental conditions.

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Section: Introductionmentioning

confidence: 99%

Response to Salt Stress in Lettuce: Changes in Chlorophyll Fluorescence Parameters, Phytochemical Contents, and Antioxidant Activities

et al. 2020

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“…Fluorescence imaging (FI) commonly utilizes a LED or laser light source to assess photosynthetic electron transfer (Bauriegel et al 2014), and photosynthetic activity can be measured by a variety of chlorophyll fluorescence parameters. For disease detection, the empirical fluorescence parameter Fv/F0 has been proposed for use on dark adapted plants (Kuckenberg et al 2007).…”

Section: Fluorescence Imagingmentioning

confidence: 99%

Plant pests and disease detection using optical sensors / Daljinsko zaznavanje rastlinskih bolezni in škodljivcev

Žibrat¹,

Knapič²,

Urek³

2019

FBG

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Plant pests and disease detection using optical sensors Traditional agricultural plant pest and disease management practices are based on visible characteristics and require that plants are checked individually, making these practices time consuming and therefore costly. Plant pests and diseases also often exhibit a heterogeneous distribution, making detection more difficult. Remote sensing methods enable comparatively accurate detection of pests and diseases over larger areas. Furthermore, because remote sensing sensors utilize light outside the human visible spectrum, presymptomatic detection becomes possible, thus facilitating timely, appropriate and spatially accurate management practices. Because remote sensing systems generate large amount of data, novel data analysis methods, such as machine learning, were introduced to plant protection. While pest and disease detection is possible using individual sensors, best results can be obtained by combining different sensors, utilizing different spectral ranges or physiological responses to light. A large amount of data and information has been generated in the past, but this research has mostly been focused on individual pathogens. Future research will have to focus on combined infections or infestations, and include abiotic stressors as well.

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“…Changes in the photosynthetic parameters were also visualized during host resistance [38,55]. A Chl-FI approach of pixel-wise analyses of F V /F M was capable of distinguishing resistant and susceptible butterhead and batavia lettuce lines against Bremia lactucae [56].…”

Section: Chlorophyll Fluorescence Imaging To Monitor Plants Infected mentioning

confidence: 99%

Picturing pathogen infection in plants

Barón

Pineda

Pérez-Bueno

2016

Zeitschrift Für Naturforschung C

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Several imaging techniques have provided valuable tools to evaluate the impact of biotic stress on host plants. The use of these techniques enables the study of plant-pathogen interactions by analysing the spatial and temporal heterogeneity of foliar metabolism during pathogenesis. In this work we review the use of imaging techniques based on chlorophyll fluorescence, multicolour fluorescence and thermography for the study of virus, bacteria and fungi-infected plants. These studies have revealed the impact of pathogen challenge on photosynthetic performance, secondary metabolism, as well as leaf transpiration as a promising tool for field and greenhouse management of diseases. Images of standard chlorophyll fluorescence (Chl-F) parameters obtained during Chl-F induction kinetics related to photochemical processes and those involved in energy dissipation, could be good stress indicators to monitor pathogenesis. Changes on UV-induced blue (F440) and green fluorescence (F520) measured by multicolour fluorescence imaging in pathogen-challenged plants seem to be related with the up-regulation of the plant secondary metabolism and with an increase in phenolic compounds involved in plant defence, such as scopoletin, chlorogenic or ferulic acids. Thermal imaging visualizes the leaf transpiration map during pathogenesis and emphasizes the key role of stomata on innate plant immunity. Using several imaging techniques in parallel could allow obtaining disease signatures for a specific pathogen. These techniques have also turned out to be very useful for presymptomatic pathogen detection, and powerful non-destructive tools for precision agriculture. Their applicability at lab-scale, in the field by remote sensing, and in high-throughput plant phenotyping, makes them particularly useful. Thermal sensors are widely used in crop fields to detect early changes in leaf transpiration induced by both air-borne and soil-borne pathogens. The limitations of measuring photosynthesis by Chl-F at the canopy level are being solved, while the use of multispectral fluorescence imaging is very challenging due to the type of light excitation that is used.Keywords: biotic stress; chlorophyll fluorescence imaging; multicolor fluorescence imaging; plant pathogens; thermography. Dedication: This work is dedicated to the memory of Professor Peter Böger. He will always bring to my heart (M. B.) the best memories of my postdoc at Lake Constance.

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Chlorophyll fluorescence imaging to facilitate breeding of Bremia lactucae-resistant lettuce cultivars

Cited by 37 publications

References 26 publications

Response to Salt Stress in Lettuce: Changes in Chlorophyll Fluorescence Parameters, Phytochemical Contents, and Antioxidant Activities

Response to Salt Stress in Lettuce: Changes in Chlorophyll Fluorescence Parameters, Phytochemical Contents, and Antioxidant Activities

Plant pests and disease detection using optical sensors / Daljinsko zaznavanje rastlinskih bolezni in škodljivcev

Picturing pathogen infection in plants

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